Experiment: P. putida South MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2029 and PP_2030 overlap by 8 nucleotides PP_2030 and PP_2031 overlap by 4 nucleotides PP_2031 and PP_2032 overlap by 8 nucleotides
PP_2029: PP_2029 - von Willebrand factor type A domain protein, at 2,306,553 to 2,307,629
_2029
PP_2030: PP_2030 - conserved protein of unknown function, at 2,307,622 to 2,308,116
_2030
PP_2031: PP_2031 - conserved protein of unknown function, at 2,308,113 to 2,309,069
_2031
PP_2032: PP_2032 - putative ATPase, at 2,309,062 to 2,310,021
_2032
Position (kb)
2307
2308
2309 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2306.719 kb on + strand, within PP_2029 at 2306.828 kb on - strand, within PP_2029 at 2306.951 kb on - strand, within PP_2029 at 2306.951 kb on - strand, within PP_2029 at 2307.193 kb on + strand at 2307.193 kb on + strand, within PP_2029 at 2307.704 kb on + strand, within PP_2030 at 2307.705 kb on - strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.716 kb on - strand, within PP_2030 at 2307.716 kb on - strand at 2307.722 kb on - strand, within PP_2030 at 2307.722 kb on - strand, within PP_2030 at 2307.887 kb on + strand, within PP_2030 at 2308.827 kb on - strand, within PP_2031 at 2308.877 kb on - strand, within PP_2031 at 2308.877 kb on - strand, within PP_2031
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida South MOI 0.1 remove 2,306,719 + PP_2029 0.15 -1.6 2,306,828 - PP_2029 0.26 -2.8 2,306,951 - PP_2029 0.37 -3.7 2,306,951 - PP_2029 0.37 -2.5 2,307,193 + +0.7 2,307,193 + PP_2029 0.59 -2.1 2,307,704 + PP_2030 0.17 -3.4 2,307,705 - PP_2030 0.17 -0.4 2,307,715 + PP_2030 0.19 -2.3 2,307,715 + PP_2030 0.19 -1.8 2,307,715 + PP_2030 0.19 -0.0 2,307,715 + PP_2030 0.19 -2.5 2,307,715 + PP_2030 0.19 -1.0 2,307,716 - PP_2030 0.19 -3.5 2,307,716 - -1.0 2,307,722 - PP_2030 0.20 +0.3 2,307,722 - PP_2030 0.20 -3.2 2,307,887 + PP_2030 0.54 -1.3 2,308,827 - PP_2031 0.75 -1.3 2,308,877 - PP_2031 0.80 -4.1 2,308,877 - PP_2031 0.80 -3.9
Or see this region's nucleotide sequence