Experiment: P. putida South MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1728 and PP_1729 are separated by 38 nucleotides PP_1729 and PP_1730 are separated by 104 nucleotides PP_1730 and PP_1731 are separated by 170 nucleotides PP_1731 and PP_1732 are separated by 149 nucleotides
PP_1728: PP_1728 - conserved membrane protein of unknown function, at 1,927,938 to 1,930,091
_1728
PP_1729: PP_1729 - conserved protein of unknown function, at 1,930,130 to 1,930,333
_1729
PP_1730: PP_1730 - Probable M18 family aminopeptidase 2, at 1,930,438 to 1,931,727
_1730
PP_1731: PP_1731 - 23S rRNA (746) and tRNA (32) pseudouridine synthase, at 1,931,898 to 1,932,533
_1731
PP_1732: PP_1732 - Cell division topological specificity factor, at 1,932,683 to 1,932,937
_1732
Position (kb)
1930
1931
1932 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1929.496 kb on + strand, within PP_1728 at 1929.497 kb on - strand, within PP_1728 at 1929.641 kb on - strand, within PP_1728 at 1930.030 kb on - strand at 1930.123 kb on + strand at 1930.123 kb on + strand at 1930.131 kb on + strand at 1930.131 kb on + strand at 1930.131 kb on + strand at 1930.132 kb on - strand at 1930.132 kb on - strand at 1930.177 kb on + strand, within PP_1729 at 1930.206 kb on - strand, within PP_1729 at 1930.434 kb on + strand at 1930.435 kb on - strand at 1930.435 kb on - strand at 1930.460 kb on + strand at 1930.460 kb on + strand at 1930.460 kb on + strand at 1930.474 kb on + strand at 1930.474 kb on + strand at 1930.475 kb on - strand at 1930.475 kb on - strand at 1930.645 kb on + strand, within PP_1730 at 1930.847 kb on + strand, within PP_1730 at 1930.848 kb on - strand, within PP_1730 at 1931.084 kb on + strand, within PP_1730 at 1931.084 kb on + strand, within PP_1730 at 1931.085 kb on - strand, within PP_1730 at 1931.402 kb on + strand, within PP_1730 at 1931.441 kb on - strand, within PP_1730 at 1931.570 kb on + strand, within PP_1730 at 1931.570 kb on + strand, within PP_1730 at 1931.571 kb on - strand, within PP_1730 at 1931.571 kb on - strand, within PP_1730 at 1931.571 kb on - strand, within PP_1730 at 1931.573 kb on + strand, within PP_1730 at 1931.574 kb on - strand, within PP_1730 at 1931.727 kb on + strand at 1931.728 kb on - strand at 1931.728 kb on - strand at 1931.818 kb on + strand at 1931.818 kb on + strand at 1931.818 kb on + strand at 1931.819 kb on - strand at 1931.864 kb on + strand at 1932.016 kb on + strand, within PP_1731 at 1932.134 kb on + strand, within PP_1731 at 1932.135 kb on - strand, within PP_1731 at 1932.135 kb on - strand, within PP_1731 at 1932.205 kb on + strand, within PP_1731 at 1932.206 kb on - strand, within PP_1731 at 1932.268 kb on + strand, within PP_1731 at 1932.268 kb on + strand, within PP_1731 at 1932.269 kb on - strand, within PP_1731 at 1932.269 kb on - strand, within PP_1731 at 1932.551 kb on + strand at 1932.552 kb on - strand at 1932.552 kb on - strand at 1932.552 kb on - strand at 1932.552 kb on - strand at 1932.603 kb on + strand at 1932.612 kb on + strand at 1932.613 kb on - strand at 1932.625 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida South MOI 0.1 remove 1,929,496 + PP_1728 0.72 -0.5 1,929,497 - PP_1728 0.72 -1.4 1,929,641 - PP_1728 0.79 -1.0 1,930,030 - +0.3 1,930,123 + -0.6 1,930,123 + -0.4 1,930,131 + -1.6 1,930,131 + -0.7 1,930,131 + -0.3 1,930,132 - -0.4 1,930,132 - -1.1 1,930,177 + PP_1729 0.23 +1.1 1,930,206 - PP_1729 0.37 -0.3 1,930,434 + +0.3 1,930,435 - +0.1 1,930,435 - +0.6 1,930,460 + +0.1 1,930,460 + -3.4 1,930,460 + -0.1 1,930,474 + -1.0 1,930,474 + +1.3 1,930,475 - -0.7 1,930,475 - -1.5 1,930,645 + PP_1730 0.16 -1.1 1,930,847 + PP_1730 0.32 -4.5 1,930,848 - PP_1730 0.32 -3.3 1,931,084 + PP_1730 0.50 -1.1 1,931,084 + PP_1730 0.50 +0.9 1,931,085 - PP_1730 0.50 -1.3 1,931,402 + PP_1730 0.75 -1.0 1,931,441 - PP_1730 0.78 -0.8 1,931,570 + PP_1730 0.88 -0.9 1,931,570 + PP_1730 0.88 -2.3 1,931,571 - PP_1730 0.88 -0.6 1,931,571 - PP_1730 0.88 -3.5 1,931,571 - PP_1730 0.88 -0.2 1,931,573 + PP_1730 0.88 -2.8 1,931,574 - PP_1730 0.88 -3.4 1,931,727 + -2.4 1,931,728 - -1.0 1,931,728 - -2.2 1,931,818 + +0.2 1,931,818 + -0.0 1,931,818 + +0.7 1,931,819 - +0.7 1,931,864 + +1.3 1,932,016 + PP_1731 0.19 +0.8 1,932,134 + PP_1731 0.37 -1.3 1,932,135 - PP_1731 0.37 +0.3 1,932,135 - PP_1731 0.37 +1.7 1,932,205 + PP_1731 0.48 -3.6 1,932,206 - PP_1731 0.48 -2.2 1,932,268 + PP_1731 0.58 +1.4 1,932,268 + PP_1731 0.58 +2.4 1,932,269 - PP_1731 0.58 +0.8 1,932,269 - PP_1731 0.58 -1.3 1,932,551 + -0.4 1,932,552 - +3.0 1,932,552 - -0.3 1,932,552 - -0.2 1,932,552 - -1.5 1,932,603 + -0.6 1,932,612 + -0.1 1,932,613 - -0.6 1,932,625 + +1.0
Or see this region's nucleotide sequence