Experiment: P. putida South MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1604 and PP_1605 are separated by 1 nucleotides PP_1605 and PP_1606 are separated by 186 nucleotides PP_1606 and PP_1607 are separated by 106 nucleotides
PP_1604: PP_1604 - Lipid-A-disaccharide synthase, at 1,798,078 to 1,799,205
_1604
PP_1605: PP_1605 - Ribonuclease HII, at 1,799,207 to 1,799,830
_1605
PP_1606: PP_1606 - DNA polymerase III, alpha subunit, at 1,800,017 to 1,803,541
_1606
PP_1607: PP_1607 - acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha, at 1,803,648 to 1,804,595
_1607
Position (kb)
1800
1801
1802
1803
1804 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1799.236 kb on - strand at 1799.350 kb on + strand, within PP_1605 at 1799.512 kb on + strand, within PP_1605 at 1799.513 kb on - strand, within PP_1605 at 1799.749 kb on + strand, within PP_1605 at 1799.749 kb on + strand, within PP_1605 at 1799.965 kb on + strand at 1799.965 kb on + strand at 1799.966 kb on - strand at 1799.966 kb on - strand at 1799.969 kb on + strand at 1800.004 kb on + strand at 1803.555 kb on + strand at 1803.584 kb on + strand at 1803.589 kb on + strand at 1803.628 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida South MOI 0.1 remove 1,799,236 - +1.0 1,799,350 + PP_1605 0.23 +0.1 1,799,512 + PP_1605 0.49 +2.4 1,799,513 - PP_1605 0.49 -0.6 1,799,749 + PP_1605 0.87 +0.6 1,799,749 + PP_1605 0.87 -1.2 1,799,965 + +1.6 1,799,965 + -0.8 1,799,966 - +2.8 1,799,966 - -2.0 1,799,969 + -0.2 1,800,004 + +0.6 1,803,555 + +0.8 1,803,584 + +3.1 1,803,589 + +1.5 1,803,628 + +2.1
Or see this region's nucleotide sequence