Experiment: P. putida South MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0243 and PP_0244 are separated by 153 nucleotides PP_0244 and PP_0245 overlap by 1 nucleotides PP_0245 and PP_0246 overlap by 44 nucleotides PP_0246 and PP_0247 are separated by 13 nucleotides
PP_0243: PP_0243 - Glutamate--cysteine ligase, at 296,426 to 298,003
_0243
PP_0244: PP_0244 - conserved protein of unknown function, at 298,157 to 298,540
_0244
PP_0245: PP_0245 - putative transcriptional accessory protein, at 298,540 to 301,125
_0245
PP_0246: PP_0246 - two-component system DNA-binding response transcriptional dual regulator, at 301,082 to 301,822
_0246
PP_0247: PP_0247 - Osmolarity sensor protein EnvZ, at 301,836 to 303,149
_0247
Position (kb) 298
299
300
301
302 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 298.008 kb on + strand at 298.009 kb on - strand at 298.049 kb on + strand at 298.057 kb on + strand at 298.057 kb on + strand at 298.066 kb on - strand at 298.066 kb on - strand at 298.066 kb on - strand at 298.143 kb on + strand at 298.144 kb on - strand at 298.158 kb on + strand at 298.158 kb on + strand at 298.158 kb on + strand at 298.159 kb on - strand at 298.159 kb on - strand at 298.211 kb on + strand, within PP_0244 at 298.211 kb on + strand at 298.211 kb on + strand, within PP_0244 at 298.211 kb on + strand, within PP_0244 at 298.212 kb on - strand, within PP_0244 at 298.299 kb on + strand, within PP_0244 at 298.299 kb on + strand, within PP_0244 at 298.299 kb on + strand, within PP_0244 at 298.299 kb on + strand, within PP_0244 at 298.300 kb on - strand, within PP_0244 at 298.521 kb on + strand at 298.522 kb on - strand at 298.532 kb on + strand at 298.532 kb on + strand at 298.533 kb on - strand at 298.541 kb on + strand at 298.541 kb on + strand at 298.541 kb on + strand at 298.542 kb on - strand at 298.833 kb on + strand, within PP_0245 at 298.834 kb on - strand, within PP_0245 at 298.834 kb on - strand at 298.888 kb on - strand, within PP_0245 at 299.022 kb on - strand, within PP_0245 at 299.462 kb on + strand, within PP_0245 at 299.462 kb on + strand, within PP_0245 at 299.463 kb on - strand, within PP_0245 at 299.658 kb on - strand, within PP_0245 at 299.804 kb on + strand, within PP_0245 at 299.805 kb on - strand, within PP_0245 at 300.014 kb on + strand, within PP_0245 at 300.015 kb on - strand, within PP_0245 at 300.015 kb on - strand, within PP_0245 at 300.222 kb on + strand, within PP_0245 at 300.223 kb on - strand, within PP_0245 at 300.223 kb on - strand, within PP_0245 at 300.248 kb on + strand, within PP_0245 at 300.248 kb on + strand, within PP_0245 at 300.249 kb on - strand, within PP_0245 at 300.323 kb on + strand, within PP_0245 at 300.324 kb on - strand, within PP_0245 at 300.324 kb on - strand, within PP_0245 at 300.380 kb on + strand, within PP_0245 at 300.381 kb on - strand, within PP_0245 at 300.381 kb on - strand, within PP_0245 at 300.551 kb on + strand, within PP_0245 at 300.561 kb on - strand, within PP_0245 at 300.561 kb on - strand, within PP_0245 at 300.561 kb on - strand, within PP_0245 at 300.843 kb on + strand, within PP_0245 at 300.866 kb on + strand, within PP_0245 at 300.867 kb on - strand at 300.867 kb on - strand at 300.888 kb on + strand at 300.889 kb on - strand at 300.943 kb on + strand at 300.943 kb on + strand at 300.943 kb on + strand at 300.943 kb on + strand at 300.944 kb on - strand at 300.944 kb on - strand at 300.944 kb on - strand at 300.945 kb on + strand at 300.946 kb on - strand at 300.946 kb on - strand at 300.946 kb on - strand at 301.078 kb on - strand at 301.078 kb on - strand at 301.185 kb on - strand, within PP_0246 at 301.403 kb on + strand, within PP_0246 at 301.527 kb on - strand, within PP_0246 at 301.761 kb on - strand at 301.761 kb on - strand at 301.787 kb on + strand at 301.787 kb on + strand at 301.788 kb on - strand at 301.932 kb on + strand at 301.933 kb on - strand at 301.972 kb on - strand, within PP_0247 at 301.972 kb on - strand, within PP_0247 at 302.004 kb on + strand, within PP_0247 at 302.005 kb on - strand, within PP_0247 at 302.005 kb on - strand, within PP_0247 at 302.058 kb on - strand, within PP_0247 at 302.058 kb on - strand, within PP_0247 at 302.106 kb on + strand, within PP_0247 at 302.106 kb on + strand, within PP_0247 at 302.118 kb on + strand, within PP_0247 at 302.118 kb on + strand, within PP_0247 at 302.118 kb on + strand, within PP_0247 at 302.118 kb on + strand, within PP_0247 at 302.119 kb on - strand, within PP_0247 at 302.119 kb on - strand, within PP_0247 at 302.119 kb on - strand, within PP_0247
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida South MOI 0.1 remove 298,008 + +0.4 298,009 - -0.7 298,049 + +0.4 298,057 + -0.8 298,057 + +2.4 298,066 - -1.0 298,066 - -0.9 298,066 - +0.4 298,143 + -0.4 298,144 - +0.9 298,158 + -0.2 298,158 + +0.4 298,158 + -0.6 298,159 - -0.1 298,159 - +1.0 298,211 + PP_0244 0.14 -0.6 298,211 + -2.9 298,211 + PP_0244 0.14 -0.2 298,211 + PP_0244 0.14 -0.5 298,212 - PP_0244 0.14 -1.5 298,299 + PP_0244 0.37 +0.1 298,299 + PP_0244 0.37 +0.5 298,299 + PP_0244 0.37 -0.6 298,299 + PP_0244 0.37 -0.3 298,300 - PP_0244 0.37 -0.1 298,521 + +0.2 298,522 - +1.2 298,532 + -0.3 298,532 + +0.3 298,533 - +1.0 298,541 + -0.5 298,541 + +0.2 298,541 + -0.2 298,542 - -1.2 298,833 + PP_0245 0.11 -2.8 298,834 - PP_0245 0.11 -0.5 298,834 - +1.6 298,888 - PP_0245 0.13 -0.4 299,022 - PP_0245 0.19 +0.1 299,462 + PP_0245 0.36 -2.3 299,462 + PP_0245 0.36 -1.3 299,463 - PP_0245 0.36 -2.1 299,658 - PP_0245 0.43 -0.5 299,804 + PP_0245 0.49 -1.1 299,805 - PP_0245 0.49 +0.1 300,014 + PP_0245 0.57 +0.1 300,015 - PP_0245 0.57 +0.6 300,015 - PP_0245 0.57 -3.5 300,222 + PP_0245 0.65 +0.4 300,223 - PP_0245 0.65 -4.4 300,223 - PP_0245 0.65 -2.3 300,248 + PP_0245 0.66 -2.3 300,248 + PP_0245 0.66 +0.5 300,249 - PP_0245 0.66 -0.8 300,323 + PP_0245 0.69 -1.3 300,324 - PP_0245 0.69 -0.3 300,324 - PP_0245 0.69 -0.0 300,380 + PP_0245 0.71 +1.2 300,381 - PP_0245 0.71 +0.3 300,381 - PP_0245 0.71 -0.8 300,551 + PP_0245 0.78 -0.6 300,561 - PP_0245 0.78 -0.2 300,561 - PP_0245 0.78 -1.2 300,561 - PP_0245 0.78 -0.8 300,843 + PP_0245 0.89 -0.6 300,866 + PP_0245 0.90 +1.5 300,867 - -2.0 300,867 - -0.2 300,888 + +0.3 300,889 - +0.3 300,943 + -0.0 300,943 + +0.6 300,943 + +0.8 300,943 + +0.2 300,944 - -0.2 300,944 - -0.8 300,944 - -1.2 300,945 + +1.1 300,946 - -3.4 300,946 - -2.5 300,946 - -2.2 301,078 - +1.4 301,078 - +3.0 301,185 - PP_0246 0.14 +1.3 301,403 + PP_0246 0.43 +1.8 301,527 - PP_0246 0.60 +2.5 301,761 - +0.6 301,761 - +2.2 301,787 + +2.1 301,787 + +2.2 301,788 - +0.9 301,932 + +0.7 301,933 - +2.3 301,972 - PP_0247 0.10 +1.4 301,972 - PP_0247 0.10 +2.1 302,004 + PP_0247 0.13 +1.2 302,005 - PP_0247 0.13 +1.9 302,005 - PP_0247 0.13 +2.1 302,058 - PP_0247 0.17 +1.9 302,058 - PP_0247 0.17 +1.9 302,106 + PP_0247 0.21 +1.3 302,106 + PP_0247 0.21 +1.8 302,118 + PP_0247 0.21 +0.9 302,118 + PP_0247 0.21 +1.4 302,118 + PP_0247 0.21 +0.7 302,118 + PP_0247 0.21 +0.8 302,119 - PP_0247 0.22 +1.3 302,119 - PP_0247 0.22 +1.1 302,119 - PP_0247 0.22 +0.8
Or see this region's nucleotide sequence