Experiment: P. putida South MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0066 and PP_0067 overlap by 4 nucleotides PP_0067 and PP_0068 are separated by 60 nucleotides PP_0068 and PP_0069 are separated by 206 nucleotides
PP_0066: PP_0066 - Ribosomal RNA small subunit methyltransferase B, at 76,233 to 77,543
_0066
PP_0067: PP_0067 - Methionyl-tRNA formyltransferase, at 77,540 to 78,472
_0067
PP_0068: PP_0068 - peptide deformylase, at 78,533 to 79,039
_0068
PP_0069: PP_0069 - putative Rossmann fold nucleotide-binding protein, at 79,246 to 80,343
_0069
Position (kb)
77
78
79 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 76.942 kb on + strand, within PP_0066 at 76.942 kb on + strand, within PP_0066 at 76.990 kb on + strand, within PP_0066 at 76.991 kb on - strand, within PP_0066 at 76.991 kb on - strand, within PP_0066 at 76.991 kb on - strand, within PP_0066 at 77.299 kb on + strand, within PP_0066 at 77.448 kb on + strand at 77.448 kb on + strand at 77.449 kb on - strand at 77.449 kb on - strand at 77.449 kb on - strand at 77.449 kb on - strand at 79.040 kb on - strand at 79.040 kb on - strand at 79.086 kb on - strand at 79.086 kb on - strand at 79.086 kb on - strand at 79.128 kb on - strand at 79.128 kb on - strand at 79.137 kb on - strand at 79.137 kb on - strand at 79.148 kb on - strand at 79.182 kb on - strand at 79.213 kb on + strand at 79.214 kb on - strand at 79.214 kb on - strand at 79.214 kb on - strand at 79.214 kb on - strand at 79.298 kb on + strand at 79.298 kb on + strand at 79.298 kb on + strand at 79.298 kb on + strand at 79.454 kb on + strand, within PP_0069 at 79.455 kb on - strand, within PP_0069
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida South MOI 0.1 remove 76,942 + PP_0066 0.54 -2.8 76,942 + PP_0066 0.54 -3.9 76,990 + PP_0066 0.58 -2.0 76,991 - PP_0066 0.58 -4.8 76,991 - PP_0066 0.58 -4.1 76,991 - PP_0066 0.58 -5.3 77,299 + PP_0066 0.81 -3.1 77,448 + -2.9 77,448 + -4.2 77,449 - -5.0 77,449 - -3.5 77,449 - -3.2 77,449 - -3.2 79,040 - -2.9 79,040 - -3.6 79,086 - -1.1 79,086 - -0.8 79,086 - -0.6 79,128 - -0.8 79,128 - -0.4 79,137 - +0.9 79,137 - -0.5 79,148 - -0.8 79,182 - +0.1 79,213 + -3.5 79,214 - -0.8 79,214 - +0.1 79,214 - -0.8 79,214 - -0.4 79,298 + +0.9 79,298 + -0.4 79,298 + -1.9 79,298 + -2.8 79,454 + PP_0069 0.19 -3.9 79,455 - PP_0069 0.19 -1.9
Or see this region's nucleotide sequence