Strain Fitness in Pseudomonas putida KT2440 around PP_0067

Experiment: P. putida South MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0066 and PP_0067 overlap by 4 nucleotidesPP_0067 and PP_0068 are separated by 60 nucleotidesPP_0068 and PP_0069 are separated by 206 nucleotides PP_0066: PP_0066 - Ribosomal RNA small subunit methyltransferase B, at 76,233 to 77,543 _0066 PP_0067: PP_0067 - Methionyl-tRNA formyltransferase, at 77,540 to 78,472 _0067 PP_0068: PP_0068 - peptide deformylase, at 78,533 to 79,039 _0068 PP_0069: PP_0069 - putative Rossmann fold nucleotide-binding protein, at 79,246 to 80,343 _0069 Position (kb) 77 78 79Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 76.942 kb on + strand, within PP_0066at 76.942 kb on + strand, within PP_0066at 76.990 kb on + strand, within PP_0066at 76.991 kb on - strand, within PP_0066at 76.991 kb on - strand, within PP_0066at 76.991 kb on - strand, within PP_0066at 77.299 kb on + strand, within PP_0066at 77.448 kb on + strandat 77.448 kb on + strandat 77.449 kb on - strandat 77.449 kb on - strandat 77.449 kb on - strandat 77.449 kb on - strandat 79.040 kb on - strandat 79.040 kb on - strandat 79.086 kb on - strandat 79.086 kb on - strandat 79.086 kb on - strandat 79.128 kb on - strandat 79.128 kb on - strandat 79.137 kb on - strandat 79.137 kb on - strandat 79.148 kb on - strandat 79.182 kb on - strandat 79.213 kb on + strandat 79.214 kb on - strandat 79.214 kb on - strandat 79.214 kb on - strandat 79.214 kb on - strandat 79.298 kb on + strandat 79.298 kb on + strandat 79.298 kb on + strandat 79.298 kb on + strandat 79.454 kb on + strand, within PP_0069at 79.455 kb on - strand, within PP_0069

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Per-strain Table

Position Strand Gene LocusTag Fraction P. putida South MOI 0.1
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76,942 + PP_0066 0.54 -2.8
76,942 + PP_0066 0.54 -3.9
76,990 + PP_0066 0.58 -2.0
76,991 - PP_0066 0.58 -4.8
76,991 - PP_0066 0.58 -4.1
76,991 - PP_0066 0.58 -5.3
77,299 + PP_0066 0.81 -3.1
77,448 + -2.9
77,448 + -4.2
77,449 - -5.0
77,449 - -3.5
77,449 - -3.2
77,449 - -3.2
79,040 - -2.9
79,040 - -3.6
79,086 - -1.1
79,086 - -0.8
79,086 - -0.6
79,128 - -0.8
79,128 - -0.4
79,137 - +0.9
79,137 - -0.5
79,148 - -0.8
79,182 - +0.1
79,213 + -3.5
79,214 - -0.8
79,214 - +0.1
79,214 - -0.8
79,214 - -0.4
79,298 + +0.9
79,298 + -0.4
79,298 + -1.9
79,298 + -2.8
79,454 + PP_0069 0.19 -3.9
79,455 - PP_0069 0.19 -1.9

Or see this region's nucleotide sequence