Experiment: P. putida South MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0061 and PP_0062 are separated by 83 nucleotides PP_0062 and PP_0063 are separated by 41 nucleotides
PP_0061: PP_0061 - glycine-tRNA ligase alpha subunit, at 71,901 to 72,848
_0061
PP_0062: PP_0062 - 3-methyl-adenine DNA glycosylase I, at 72,932 to 73,483
_0062
PP_0063: PP_0063 - putative Lipid A biosynthesis lauroyl acyltransferase, at 73,525 to 74,412
_0063
Position (kb) 72
73
74 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 72.893 kb on - strand at 72.893 kb on - strand at 72.893 kb on - strand at 72.895 kb on - strand at 72.917 kb on - strand at 72.932 kb on - strand at 72.932 kb on - strand at 72.969 kb on - strand at 73.073 kb on - strand, within PP_0062 at 73.073 kb on - strand, within PP_0062 at 73.112 kb on + strand, within PP_0062 at 73.112 kb on + strand, within PP_0062 at 73.113 kb on - strand, within PP_0062 at 73.113 kb on - strand, within PP_0062 at 73.113 kb on - strand, within PP_0062 at 73.113 kb on - strand, within PP_0062 at 73.128 kb on + strand, within PP_0062 at 73.406 kb on + strand, within PP_0062 at 73.406 kb on + strand, within PP_0062 at 73.407 kb on - strand, within PP_0062 at 73.489 kb on + strand at 73.489 kb on + strand at 73.490 kb on - strand at 73.490 kb on - strand at 73.490 kb on - strand at 73.512 kb on - strand at 73.662 kb on - strand, within PP_0063 at 73.746 kb on + strand at 73.747 kb on - strand, within PP_0063 at 73.858 kb on - strand, within PP_0063 at 73.915 kb on + strand, within PP_0063 at 73.915 kb on + strand, within PP_0063 at 73.915 kb on + strand, within PP_0063 at 73.915 kb on + strand, within PP_0063 at 73.916 kb on - strand, within PP_0063 at 74.082 kb on + strand, within PP_0063 at 74.195 kb on + strand, within PP_0063 at 74.196 kb on - strand, within PP_0063 at 74.275 kb on + strand, within PP_0063 at 74.275 kb on + strand, within PP_0063 at 74.275 kb on + strand, within PP_0063 at 74.276 kb on - strand, within PP_0063 at 74.276 kb on - strand, within PP_0063 at 74.276 kb on - strand, within PP_0063 at 74.276 kb on - strand, within PP_0063 at 74.276 kb on - strand, within PP_0063 at 74.329 kb on + strand at 74.330 kb on - strand at 74.330 kb on - strand at 74.330 kb on - strand at 74.330 kb on - strand at 74.341 kb on + strand at 74.353 kb on + strand at 74.353 kb on + strand at 74.354 kb on - strand at 74.354 kb on - strand at 74.354 kb on - strand at 74.410 kb on + strand at 74.410 kb on + strand at 74.411 kb on - strand at 74.411 kb on - strand at 74.411 kb on - strand at 74.411 kb on - strand at 74.411 kb on - strand at 74.411 kb on - strand at 74.431 kb on + strand at 74.431 kb on + strand at 74.431 kb on + strand at 74.431 kb on + strand at 74.431 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida South MOI 1 remove 72,893 - -0.4 72,893 - -0.3 72,893 - +0.2 72,895 - -1.1 72,917 - +0.0 72,932 - -0.4 72,932 - -0.9 72,969 - -0.7 73,073 - PP_0062 0.26 +1.4 73,073 - PP_0062 0.26 +1.3 73,112 + PP_0062 0.33 +0.4 73,112 + PP_0062 0.33 +1.2 73,113 - PP_0062 0.33 -0.8 73,113 - PP_0062 0.33 -1.8 73,113 - PP_0062 0.33 +0.7 73,113 - PP_0062 0.33 +0.0 73,128 + PP_0062 0.36 +2.6 73,406 + PP_0062 0.86 -1.3 73,406 + PP_0062 0.86 +0.1 73,407 - PP_0062 0.86 +1.1 73,489 + -0.2 73,489 + +0.2 73,490 - +2.8 73,490 - +3.0 73,490 - +3.1 73,512 - +0.5 73,662 - PP_0063 0.15 +2.7 73,746 + +3.1 73,747 - PP_0063 0.25 +3.4 73,858 - PP_0063 0.38 +3.0 73,915 + PP_0063 0.44 +3.7 73,915 + PP_0063 0.44 +2.6 73,915 + PP_0063 0.44 +3.0 73,915 + PP_0063 0.44 +2.1 73,916 - PP_0063 0.44 +3.8 74,082 + PP_0063 0.63 +1.1 74,195 + PP_0063 0.75 +2.5 74,196 - PP_0063 0.76 +0.5 74,275 + PP_0063 0.84 +3.1 74,275 + PP_0063 0.84 -0.1 74,275 + PP_0063 0.84 +3.3 74,276 - PP_0063 0.85 +1.6 74,276 - PP_0063 0.85 +2.3 74,276 - PP_0063 0.85 +3.0 74,276 - PP_0063 0.85 +2.9 74,276 - PP_0063 0.85 +3.5 74,329 + +3.2 74,330 - +2.3 74,330 - +2.4 74,330 - +1.4 74,330 - +1.7 74,341 + +2.6 74,353 + +2.5 74,353 + +2.3 74,354 - +4.8 74,354 - +2.1 74,354 - +1.9 74,410 + +0.5 74,410 + +0.8 74,411 - +0.3 74,411 - -0.1 74,411 - -0.6 74,411 - +0.6 74,411 - -0.2 74,411 - +0.0 74,431 + +0.6 74,431 + -0.3 74,431 + +0.2 74,431 + +0.5 74,431 + +1.7
Or see this region's nucleotide sequence