Experiment: P. putida South MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_23SB and PP_r01 are separated by 132 nucleotides PP_r01 and PP_5SB are separated by 105 nucleotides PP_5SB and PP_0160 are separated by 64 nucleotides
PP_23SB: PP_23SB - 23S ribosomal RNA, at 178,633 to 181,534
_23SB
PP_r01: PP_r01 - 5S ribosomal RNA, at 181,667 to 181,786
_r01
PP_5SB: PP_5SB - 5S ribosomal RNA, at 181,892 to 182,011
_5SB
PP_0160: PP_0160 - putative ferrioxamine receptor, at 182,076 to 184,562
_0160
Position (kb) 181
182
183 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 181.838 kb on + strand at 181.906 kb on - strand, within PP_5SB at 181.906 kb on - strand, within PP_5SB at 181.994 kb on + strand, within PP_5SB at 182.077 kb on + strand at 182.078 kb on - strand at 182.086 kb on + strand at 182.087 kb on - strand at 182.125 kb on + strand at 182.126 kb on - strand at 182.126 kb on - strand at 182.126 kb on - strand at 182.126 kb on - strand at 182.126 kb on - strand at 182.132 kb on - strand at 182.158 kb on + strand at 182.158 kb on + strand at 182.158 kb on + strand at 182.158 kb on + strand at 182.158 kb on + strand at 182.158 kb on + strand at 182.159 kb on - strand at 182.159 kb on - strand at 182.159 kb on - strand at 182.171 kb on + strand at 182.171 kb on - strand at 182.172 kb on - strand at 182.172 kb on - strand at 182.231 kb on - strand at 182.257 kb on + strand at 182.257 kb on + strand at 182.258 kb on - strand at 182.258 kb on - strand at 182.258 kb on - strand at 182.308 kb on + strand at 182.308 kb on + strand at 182.309 kb on - strand at 182.309 kb on - strand at 182.309 kb on - strand at 182.309 kb on - strand at 182.360 kb on + strand, within PP_0160 at 182.360 kb on + strand, within PP_0160 at 182.362 kb on + strand, within PP_0160 at 182.362 kb on + strand, within PP_0160 at 182.362 kb on + strand, within PP_0160 at 182.362 kb on + strand, within PP_0160 at 182.363 kb on - strand, within PP_0160 at 182.411 kb on + strand, within PP_0160 at 182.411 kb on + strand, within PP_0160 at 182.411 kb on + strand, within PP_0160 at 182.462 kb on - strand, within PP_0160 at 182.480 kb on + strand, within PP_0160 at 182.480 kb on + strand, within PP_0160 at 182.480 kb on + strand, within PP_0160 at 182.480 kb on + strand, within PP_0160 at 182.481 kb on - strand, within PP_0160 at 182.481 kb on - strand, within PP_0160 at 182.481 kb on - strand, within PP_0160 at 182.481 kb on - strand, within PP_0160 at 182.482 kb on + strand, within PP_0160 at 182.483 kb on - strand, within PP_0160 at 182.483 kb on - strand, within PP_0160 at 182.569 kb on + strand, within PP_0160 at 182.569 kb on + strand, within PP_0160 at 182.569 kb on + strand, within PP_0160 at 182.570 kb on - strand, within PP_0160 at 182.662 kb on + strand, within PP_0160 at 182.662 kb on + strand, within PP_0160 at 182.663 kb on - strand, within PP_0160 at 182.663 kb on - strand, within PP_0160 at 182.663 kb on - strand, within PP_0160 at 182.792 kb on - strand, within PP_0160 at 182.792 kb on - strand, within PP_0160 at 182.985 kb on + strand, within PP_0160 at 183.007 kb on + strand, within PP_0160
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida South MOI 10 remove 181,838 + +1.1 181,906 - PP_5SB 0.12 -1.1 181,906 - PP_5SB 0.12 -0.5 181,994 + PP_5SB 0.85 -1.3 182,077 + +0.2 182,078 - +1.9 182,086 + +0.7 182,087 - +1.0 182,125 + -0.5 182,126 - -0.3 182,126 - -0.2 182,126 - -1.0 182,126 - +1.5 182,126 - +0.5 182,132 - -1.5 182,158 + +1.0 182,158 + +2.1 182,158 + -0.0 182,158 + -1.1 182,158 + +1.3 182,158 + +0.6 182,159 - -0.5 182,159 - -0.5 182,159 - -1.1 182,171 + -0.5 182,171 - +0.1 182,172 - -1.3 182,172 - +0.8 182,231 - +1.8 182,257 + -0.2 182,257 + -0.2 182,258 - -0.5 182,258 - +3.4 182,258 - +0.7 182,308 + +0.2 182,308 + +0.9 182,309 - +0.6 182,309 - -0.5 182,309 - +0.3 182,309 - -0.2 182,360 + PP_0160 0.11 -0.7 182,360 + PP_0160 0.11 +0.4 182,362 + PP_0160 0.11 +0.8 182,362 + PP_0160 0.11 -0.2 182,362 + PP_0160 0.11 -1.9 182,362 + PP_0160 0.11 +0.4 182,363 - PP_0160 0.12 +1.0 182,411 + PP_0160 0.13 +0.3 182,411 + PP_0160 0.13 -1.4 182,411 + PP_0160 0.13 +0.7 182,462 - PP_0160 0.16 +0.0 182,480 + PP_0160 0.16 +0.9 182,480 + PP_0160 0.16 -0.3 182,480 + PP_0160 0.16 +0.5 182,480 + PP_0160 0.16 +0.1 182,481 - PP_0160 0.16 -0.3 182,481 - PP_0160 0.16 -4.0 182,481 - PP_0160 0.16 -0.3 182,481 - PP_0160 0.16 -0.3 182,482 + PP_0160 0.16 +0.9 182,483 - PP_0160 0.16 +0.1 182,483 - PP_0160 0.16 -1.0 182,569 + PP_0160 0.20 -1.1 182,569 + PP_0160 0.20 +1.3 182,569 + PP_0160 0.20 +1.2 182,570 - PP_0160 0.20 -2.4 182,662 + PP_0160 0.24 -0.4 182,662 + PP_0160 0.24 -0.6 182,663 - PP_0160 0.24 +2.0 182,663 - PP_0160 0.24 +0.9 182,663 - PP_0160 0.24 +0.5 182,792 - PP_0160 0.29 +0.8 182,792 - PP_0160 0.29 -0.2 182,985 + PP_0160 0.37 +1.0 183,007 + PP_0160 0.37 +0.9
Or see this region's nucleotide sequence