Experiment: P. putida South MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1475 and PP_1476 are separated by 130 nucleotides PP_1476 and PP_1477 are separated by 8 nucleotides PP_1477 and PP_1478 are separated by 230 nucleotides
PP_1475: PP_1475 - CAIB/BAIF family protein, at 1,679,601 to 1,680,800
_1475
PP_1476: PP_1476 - conserved protein of unknown function, at 1,680,931 to 1,681,473
_1476
PP_1477: PP_1477 - ssDNA-specific exonuclease RecJ, at 1,681,482 to 1,683,191
_1477
PP_1478: PP_1478 - putative Xenobiotic reductase, at 1,683,422 to 1,684,528
_1478
Position (kb)
1681
1682
1683
1684 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1680.638 kb on - strand, within PP_1475 at 1680.767 kb on + strand at 1680.768 kb on - strand at 1680.893 kb on - strand at 1680.953 kb on - strand at 1681.069 kb on + strand, within PP_1476 at 1681.069 kb on + strand, within PP_1476 at 1681.070 kb on - strand, within PP_1476 at 1681.082 kb on - strand, within PP_1476 at 1681.250 kb on - strand, within PP_1476 at 1681.276 kb on - strand, within PP_1476 at 1681.369 kb on - strand, within PP_1476 at 1681.369 kb on - strand, within PP_1476 at 1681.402 kb on - strand, within PP_1476 at 1681.402 kb on - strand at 1681.620 kb on + strand at 1681.783 kb on - strand, within PP_1477 at 1681.783 kb on - strand, within PP_1477 at 1681.813 kb on - strand, within PP_1477 at 1682.058 kb on + strand, within PP_1477 at 1682.058 kb on + strand, within PP_1477 at 1682.058 kb on + strand, within PP_1477 at 1682.059 kb on - strand, within PP_1477 at 1682.059 kb on - strand, within PP_1477 at 1682.059 kb on - strand, within PP_1477 at 1682.104 kb on - strand, within PP_1477 at 1682.524 kb on - strand, within PP_1477 at 1682.540 kb on - strand, within PP_1477 at 1682.570 kb on + strand, within PP_1477 at 1682.598 kb on + strand, within PP_1477 at 1682.679 kb on - strand, within PP_1477 at 1682.777 kb on + strand, within PP_1477 at 1683.117 kb on + strand at 1683.176 kb on + strand at 1683.177 kb on - strand at 1683.177 kb on - strand at 1683.177 kb on - strand at 1683.334 kb on + strand at 1683.344 kb on - strand at 1683.443 kb on + strand at 1683.443 kb on + strand at 1683.745 kb on + strand, within PP_1478 at 1683.746 kb on - strand, within PP_1478 at 1683.746 kb on - strand, within PP_1478 at 1683.746 kb on - strand, within PP_1478 at 1683.746 kb on - strand, within PP_1478 at 1683.749 kb on + strand, within PP_1478 at 1683.966 kb on - strand, within PP_1478 at 1684.016 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida South MOI 10 remove 1,680,638 - PP_1475 0.86 +1.5 1,680,767 + -1.8 1,680,768 - +0.9 1,680,893 - -0.9 1,680,953 - +0.2 1,681,069 + PP_1476 0.25 +1.4 1,681,069 + PP_1476 0.25 -0.6 1,681,070 - PP_1476 0.26 -0.4 1,681,082 - PP_1476 0.28 -0.4 1,681,250 - PP_1476 0.59 -0.5 1,681,276 - PP_1476 0.64 -1.7 1,681,369 - PP_1476 0.81 +0.8 1,681,369 - PP_1476 0.81 -1.9 1,681,402 - PP_1476 0.87 +0.0 1,681,402 - +1.8 1,681,620 + -0.3 1,681,783 - PP_1477 0.18 -0.4 1,681,783 - PP_1477 0.18 +0.3 1,681,813 - PP_1477 0.19 -1.4 1,682,058 + PP_1477 0.34 +0.2 1,682,058 + PP_1477 0.34 +0.1 1,682,058 + PP_1477 0.34 +0.1 1,682,059 - PP_1477 0.34 +0.6 1,682,059 - PP_1477 0.34 +0.4 1,682,059 - PP_1477 0.34 -1.5 1,682,104 - PP_1477 0.36 +0.8 1,682,524 - PP_1477 0.61 +0.3 1,682,540 - PP_1477 0.62 -2.6 1,682,570 + PP_1477 0.64 +1.5 1,682,598 + PP_1477 0.65 +0.1 1,682,679 - PP_1477 0.70 +1.0 1,682,777 + PP_1477 0.76 -0.5 1,683,117 + -0.4 1,683,176 + +0.4 1,683,177 - +0.7 1,683,177 - -1.1 1,683,177 - +0.9 1,683,334 + -0.5 1,683,344 - +0.8 1,683,443 + -0.4 1,683,443 + +0.2 1,683,745 + PP_1478 0.29 +0.4 1,683,746 - PP_1478 0.29 -0.7 1,683,746 - PP_1478 0.29 +0.4 1,683,746 - PP_1478 0.29 +0.0 1,683,746 - PP_1478 0.29 -0.3 1,683,749 + PP_1478 0.30 +0.8 1,683,966 - PP_1478 0.49 +0.6 1,684,016 + +0.8
Or see this region's nucleotide sequence