Experiment: P. putida South MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2525 and PP_2526 are separated by 146 nucleotides PP_2526 and PP_2527 are separated by 81 nucleotides PP_2527 and PP_2528 are separated by 64 nucleotides
PP_2525: PP_2525 - conserved protein of unknown function, at 2,871,911 to 2,873,074
_2525
PP_2526: PP_2526 - conserved membrane protein of unknown function, at 2,873,221 to 2,873,694
_2526
PP_2527: PP_2527 - conserved membrane protein of unknown function, at 2,873,776 to 2,874,540
_2527
PP_2528: PP_2528 - O-acetylhomoserine (thiol)-lyase, at 2,874,605 to 2,875,882
_2528
Position (kb)
2873
2874
2875 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2873.046 kb on + strand at 2873.065 kb on + strand at 2873.066 kb on - strand at 2873.183 kb on + strand at 2873.183 kb on + strand at 2873.183 kb on + strand at 2873.184 kb on - strand at 2873.185 kb on + strand at 2873.186 kb on - strand at 2873.186 kb on - strand at 2873.448 kb on - strand, within PP_2526 at 2873.596 kb on + strand, within PP_2526 at 2873.596 kb on + strand, within PP_2526 at 2873.605 kb on + strand, within PP_2526 at 2873.665 kb on + strand at 2873.666 kb on - strand at 2873.666 kb on - strand at 2873.666 kb on - strand at 2873.891 kb on + strand, within PP_2527 at 2873.891 kb on + strand, within PP_2527 at 2874.086 kb on - strand, within PP_2527 at 2874.086 kb on - strand, within PP_2527 at 2874.135 kb on + strand, within PP_2527 at 2874.209 kb on + strand, within PP_2527 at 2874.210 kb on - strand, within PP_2527 at 2874.210 kb on - strand, within PP_2527 at 2874.251 kb on + strand, within PP_2527 at 2874.251 kb on + strand, within PP_2527 at 2874.252 kb on - strand, within PP_2527 at 2874.252 kb on - strand, within PP_2527 at 2874.320 kb on + strand, within PP_2527 at 2874.321 kb on - strand, within PP_2527 at 2874.321 kb on - strand, within PP_2527 at 2874.528 kb on - strand at 2874.797 kb on + strand, within PP_2528 at 2874.972 kb on + strand, within PP_2528 at 2874.972 kb on + strand, within PP_2528 at 2874.973 kb on - strand, within PP_2528 at 2874.987 kb on - strand, within PP_2528 at 2875.158 kb on + strand, within PP_2528 at 2875.269 kb on + strand, within PP_2528 at 2875.269 kb on + strand at 2875.408 kb on + strand, within PP_2528 at 2875.409 kb on - strand at 2875.442 kb on + strand, within PP_2528 at 2875.443 kb on - strand, within PP_2528
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida South MOI 10 remove 2,873,046 + +1.1 2,873,065 + +1.4 2,873,066 - +1.0 2,873,183 + -1.3 2,873,183 + -0.5 2,873,183 + -0.4 2,873,184 - -0.8 2,873,185 + -1.0 2,873,186 - +3.1 2,873,186 - +0.0 2,873,448 - PP_2526 0.48 -0.9 2,873,596 + PP_2526 0.79 +0.8 2,873,596 + PP_2526 0.79 +0.4 2,873,605 + PP_2526 0.81 +0.8 2,873,665 + +0.3 2,873,666 - +0.5 2,873,666 - +1.0 2,873,666 - +1.1 2,873,891 + PP_2527 0.15 +0.1 2,873,891 + PP_2527 0.15 -0.3 2,874,086 - PP_2527 0.41 -0.0 2,874,086 - PP_2527 0.41 -0.4 2,874,135 + PP_2527 0.47 +0.1 2,874,209 + PP_2527 0.57 -0.6 2,874,210 - PP_2527 0.57 -0.5 2,874,210 - PP_2527 0.57 +0.7 2,874,251 + PP_2527 0.62 -0.3 2,874,251 + PP_2527 0.62 -1.6 2,874,252 - PP_2527 0.62 +0.3 2,874,252 - PP_2527 0.62 -3.3 2,874,320 + PP_2527 0.71 -3.5 2,874,321 - PP_2527 0.71 -1.0 2,874,321 - PP_2527 0.71 -1.6 2,874,528 - -1.2 2,874,797 + PP_2528 0.15 +0.9 2,874,972 + PP_2528 0.29 -3.2 2,874,972 + PP_2528 0.29 +1.0 2,874,973 - PP_2528 0.29 +0.9 2,874,987 - PP_2528 0.30 -3.8 2,875,158 + PP_2528 0.43 +0.4 2,875,269 + PP_2528 0.52 -3.0 2,875,269 + +1.5 2,875,408 + PP_2528 0.63 +0.1 2,875,409 - -0.1 2,875,442 + PP_2528 0.65 -4.4 2,875,443 - PP_2528 0.66 -0.4
Or see this region's nucleotide sequence