Experiment: P. putida South MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0771 and PP_0772 are separated by 18 nucleotides PP_0772 and PP_0773 are separated by 118 nucleotides PP_0773 and PP_0774 are separated by 79 nucleotides
PP_0771: PP_0771 - mRNA interferase, at 889,708 to 890,055
_0771
PP_0772: PP_0772 - Metallo-beta-lactamase family protein, at 890,074 to 890,718
_0772
PP_0773: PP_0773 - OmpA/MotB domain-containing protein, at 890,837 to 891,547
_0773
PP_0774: PP_0774 - Phosphate acetyltransferase, at 891,627 to 893,714
_0774
Position (kb) 890
891
892 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 889.844 kb on + strand, within PP_0771 at 889.845 kb on - strand, within PP_0771 at 889.845 kb on - strand, within PP_0771 at 889.991 kb on + strand, within PP_0771 at 889.992 kb on - strand, within PP_0771 at 889.992 kb on - strand, within PP_0771 at 890.067 kb on - strand at 890.079 kb on + strand at 890.079 kb on + strand at 890.079 kb on + strand at 890.079 kb on + strand at 890.080 kb on - strand at 890.080 kb on - strand at 890.392 kb on + strand, within PP_0772 at 890.392 kb on + strand, within PP_0772 at 890.393 kb on - strand, within PP_0772 at 890.393 kb on - strand, within PP_0772 at 890.393 kb on - strand, within PP_0772 at 890.469 kb on + strand, within PP_0772 at 890.469 kb on + strand, within PP_0772 at 890.470 kb on - strand, within PP_0772 at 890.470 kb on - strand, within PP_0772 at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.778 kb on - strand at 890.778 kb on - strand at 890.778 kb on - strand at 890.778 kb on - strand at 890.778 kb on - strand at 890.816 kb on - strand at 890.903 kb on + strand at 890.904 kb on - strand at 890.957 kb on + strand, within PP_0773 at 891.080 kb on + strand, within PP_0773 at 891.224 kb on + strand, within PP_0773 at 891.365 kb on + strand, within PP_0773 at 891.365 kb on + strand, within PP_0773 at 891.366 kb on - strand, within PP_0773 at 891.366 kb on - strand, within PP_0773 at 891.366 kb on - strand, within PP_0773 at 891.366 kb on - strand, within PP_0773 at 891.405 kb on + strand, within PP_0773 at 891.406 kb on - strand, within PP_0773 at 891.512 kb on + strand at 891.512 kb on + strand at 891.512 kb on + strand at 891.513 kb on - strand at 891.513 kb on - strand at 891.540 kb on - strand at 891.555 kb on + strand at 891.556 kb on - strand at 891.556 kb on - strand at 891.663 kb on + strand at 891.664 kb on - strand at 891.668 kb on + strand at 891.668 kb on + strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.669 kb on - strand at 891.771 kb on + strand at 891.788 kb on + strand at 891.789 kb on - strand at 891.893 kb on - strand, within PP_0774 at 891.997 kb on + strand, within PP_0774 at 891.997 kb on + strand, within PP_0774 at 891.997 kb on + strand, within PP_0774 at 891.998 kb on - strand, within PP_0774 at 892.366 kb on + strand, within PP_0774
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida South MOI 10 remove 889,844 + PP_0771 0.39 -4.3 889,845 - PP_0771 0.39 -2.4 889,845 - PP_0771 0.39 -0.9 889,991 + PP_0771 0.81 -0.1 889,992 - PP_0771 0.82 -3.4 889,992 - PP_0771 0.82 -0.4 890,067 - -3.9 890,079 + -3.0 890,079 + -2.0 890,079 + -0.4 890,079 + +0.6 890,080 - -4.2 890,080 - -4.0 890,392 + PP_0772 0.49 +0.1 890,392 + PP_0772 0.49 -1.4 890,393 - PP_0772 0.49 +0.8 890,393 - PP_0772 0.49 +1.0 890,393 - PP_0772 0.49 +1.4 890,469 + PP_0772 0.61 -0.6 890,469 + PP_0772 0.61 -0.2 890,470 - PP_0772 0.61 +0.5 890,470 - PP_0772 0.61 +0.5 890,777 + -0.4 890,777 + +1.4 890,777 + +0.4 890,777 + +1.0 890,777 + +0.8 890,777 + +0.2 890,777 + +1.9 890,777 + +1.3 890,778 - -0.9 890,778 - +2.0 890,778 - -0.3 890,778 - +0.2 890,778 - +0.6 890,816 - -2.1 890,903 + -0.5 890,904 - -0.2 890,957 + PP_0773 0.17 +1.7 891,080 + PP_0773 0.34 +1.8 891,224 + PP_0773 0.54 +0.5 891,365 + PP_0773 0.74 +0.8 891,365 + PP_0773 0.74 +1.7 891,366 - PP_0773 0.74 -0.3 891,366 - PP_0773 0.74 +0.7 891,366 - PP_0773 0.74 -0.3 891,366 - PP_0773 0.74 -3.7 891,405 + PP_0773 0.80 +1.5 891,406 - PP_0773 0.80 -2.7 891,512 + +0.5 891,512 + +0.1 891,512 + +0.4 891,513 - -1.4 891,513 - +2.8 891,540 - +0.6 891,555 + +1.4 891,556 - +0.7 891,556 - -3.9 891,663 + +1.3 891,664 - -1.6 891,668 + -0.1 891,668 + +1.6 891,669 - +2.5 891,669 - +0.5 891,669 - +1.2 891,669 - -3.7 891,669 - +2.0 891,669 - +0.9 891,669 - +0.8 891,669 - +0.2 891,771 + +0.1 891,788 + -0.0 891,789 - +1.2 891,893 - PP_0774 0.13 +0.6 891,997 + PP_0774 0.18 -0.1 891,997 + PP_0774 0.18 +1.1 891,997 + PP_0774 0.18 +1.7 891,998 - PP_0774 0.18 -2.8 892,366 + PP_0774 0.35 +0.5
Or see this region's nucleotide sequence