Experiment: P. putida Missley MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2913 and PP_2914 are separated by 158 nucleotides PP_2914 and PP_2915 are separated by 0 nucleotides PP_2915 and PP_2916 are separated by 9 nucleotides
PP_2913: PP_2913 - 5-aminolevulinate dehydratase, at 3,312,821 to 3,313,795
_2913
PP_2914: PP_2914 - osmosensory proline/betaine/H+ permease, at 3,313,954 to 3,315,456
_2914
PP_2915: PP_2915 - conserved membrane protein of unknown function, at 3,315,457 to 3,316,158
_2915
PP_2916: PP_2916 - riboflavin synthase, at 3,316,168 to 3,316,797
_2916
Position (kb)
3313
3314
3315
3316 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3313.789 kb on + strand at 3313.790 kb on - strand at 3313.898 kb on - strand at 3313.898 kb on - strand at 3313.902 kb on - strand at 3313.915 kb on - strand at 3313.926 kb on + strand at 3314.084 kb on + strand at 3314.085 kb on - strand at 3314.085 kb on - strand at 3314.086 kb on + strand at 3314.087 kb on - strand at 3314.087 kb on - strand at 3314.101 kb on + strand at 3314.102 kb on - strand at 3314.303 kb on + strand, within PP_2914 at 3314.311 kb on + strand, within PP_2914 at 3314.312 kb on - strand, within PP_2914 at 3314.312 kb on - strand, within PP_2914 at 3314.325 kb on + strand, within PP_2914 at 3314.389 kb on + strand, within PP_2914 at 3314.390 kb on - strand, within PP_2914 at 3314.390 kb on - strand, within PP_2914 at 3314.486 kb on + strand, within PP_2914 at 3314.486 kb on + strand, within PP_2914 at 3314.780 kb on - strand, within PP_2914 at 3314.780 kb on - strand, within PP_2914 at 3314.782 kb on + strand, within PP_2914 at 3314.782 kb on + strand, within PP_2914 at 3314.782 kb on + strand, within PP_2914 at 3314.783 kb on - strand, within PP_2914 at 3314.783 kb on - strand, within PP_2914 at 3314.783 kb on - strand, within PP_2914 at 3314.783 kb on - strand, within PP_2914 at 3314.794 kb on - strand, within PP_2914 at 3314.794 kb on - strand, within PP_2914 at 3314.797 kb on + strand, within PP_2914 at 3314.797 kb on + strand, within PP_2914 at 3314.797 kb on + strand, within PP_2914 at 3314.797 kb on + strand, within PP_2914 at 3314.797 kb on + strand, within PP_2914 at 3314.798 kb on - strand, within PP_2914 at 3314.798 kb on - strand, within PP_2914 at 3314.809 kb on + strand, within PP_2914 at 3314.830 kb on + strand, within PP_2914 at 3315.008 kb on + strand, within PP_2914 at 3315.008 kb on + strand, within PP_2914 at 3315.009 kb on - strand, within PP_2914 at 3315.016 kb on - strand, within PP_2914 at 3315.112 kb on + strand, within PP_2914 at 3315.208 kb on + strand, within PP_2914 at 3315.208 kb on + strand, within PP_2914 at 3315.208 kb on + strand, within PP_2914 at 3315.209 kb on - strand, within PP_2914 at 3315.224 kb on - strand, within PP_2914 at 3315.471 kb on - strand at 3315.471 kb on - strand at 3315.471 kb on - strand at 3315.704 kb on + strand, within PP_2915 at 3315.994 kb on - strand at 3316.106 kb on - strand at 3316.197 kb on + strand at 3316.216 kb on + strand at 3316.217 kb on - strand at 3316.280 kb on + strand, within PP_2916 at 3316.281 kb on - strand, within PP_2916 at 3316.281 kb on - strand, within PP_2916 at 3316.403 kb on + strand, within PP_2916 at 3316.403 kb on + strand, within PP_2916 at 3316.403 kb on + strand, within PP_2916 at 3316.404 kb on - strand, within PP_2916
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida Missley MOI 0.1 remove 3,313,789 + +1.6 3,313,790 - -1.8 3,313,898 - -1.6 3,313,898 - +0.7 3,313,902 - -1.4 3,313,915 - +1.7 3,313,926 + +0.2 3,314,084 + -2.1 3,314,085 - -0.2 3,314,085 - -3.3 3,314,086 + +1.7 3,314,087 - -1.4 3,314,087 - +1.4 3,314,101 + -3.7 3,314,102 - +1.6 3,314,303 + PP_2914 0.23 +0.8 3,314,311 + PP_2914 0.24 +1.6 3,314,312 - PP_2914 0.24 +0.2 3,314,312 - PP_2914 0.24 -2.8 3,314,325 + PP_2914 0.25 +1.0 3,314,389 + PP_2914 0.29 -1.1 3,314,390 - PP_2914 0.29 -3.2 3,314,390 - PP_2914 0.29 -0.2 3,314,486 + PP_2914 0.35 -0.7 3,314,486 + PP_2914 0.35 +0.2 3,314,780 - PP_2914 0.55 -0.5 3,314,780 - PP_2914 0.55 -3.0 3,314,782 + PP_2914 0.55 -0.6 3,314,782 + PP_2914 0.55 +1.3 3,314,782 + PP_2914 0.55 -2.2 3,314,783 - PP_2914 0.55 +0.0 3,314,783 - PP_2914 0.55 -2.3 3,314,783 - PP_2914 0.55 -3.1 3,314,783 - PP_2914 0.55 +0.4 3,314,794 - PP_2914 0.56 +0.6 3,314,794 - PP_2914 0.56 -0.2 3,314,797 + PP_2914 0.56 -3.7 3,314,797 + PP_2914 0.56 -1.0 3,314,797 + PP_2914 0.56 -1.3 3,314,797 + PP_2914 0.56 -3.7 3,314,797 + PP_2914 0.56 -3.0 3,314,798 - PP_2914 0.56 -0.2 3,314,798 - PP_2914 0.56 -0.1 3,314,809 + PP_2914 0.57 -0.9 3,314,830 + PP_2914 0.58 -3.7 3,315,008 + PP_2914 0.70 +0.5 3,315,008 + PP_2914 0.70 +0.5 3,315,009 - PP_2914 0.70 +1.6 3,315,016 - PP_2914 0.71 -2.5 3,315,112 + PP_2914 0.77 +1.5 3,315,208 + PP_2914 0.83 +0.1 3,315,208 + PP_2914 0.83 +2.9 3,315,208 + PP_2914 0.83 +1.0 3,315,209 - PP_2914 0.83 -0.1 3,315,224 - PP_2914 0.84 -3.1 3,315,471 - +0.5 3,315,471 - -0.1 3,315,471 - -3.4 3,315,704 + PP_2915 0.35 -0.3 3,315,994 - -0.7 3,316,106 - -1.4 3,316,197 + -0.9 3,316,216 + +1.8 3,316,217 - +2.3 3,316,280 + PP_2916 0.18 -3.1 3,316,281 - PP_2916 0.18 -2.0 3,316,281 - PP_2916 0.18 -3.9 3,316,403 + PP_2916 0.37 +0.0 3,316,403 + PP_2916 0.37 +1.0 3,316,403 + PP_2916 0.37 +1.2 3,316,404 - PP_2916 0.37 -2.9
Or see this region's nucleotide sequence