Experiment: P. putida Missley MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2846 and PP_2847 are separated by 18 nucleotides PP_2847 and PP_2848 overlap by 1 nucleotides PP_2848 and PP_2849 are separated by 11 nucleotides PP_2849 and PP_2851 are separated by 242 nucleotides
PP_2846: PP_2846 - urease accessory protein, at 3,250,094 to 3,250,597
_2846
PP_2847: PP_2847 - Urease accessory protein UreJ, at 3,250,616 to 3,251,161
_2847
PP_2848: PP_2848 - urease accessory protein, at 3,251,161 to 3,251,835
_2848
PP_2849: PP_2849 - urease accessory protein, at 3,251,847 to 3,252,470
_2849
PP_2851: PP_2851 - conserved protein of unknown function, at 3,252,713 to 3,253,441
_2851
Position (kb)
3251
3252 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3250.473 kb on + strand, within PP_2846 at 3250.473 kb on + strand, within PP_2846 at 3250.473 kb on + strand, within PP_2846 at 3250.515 kb on - strand, within PP_2846 at 3250.575 kb on + strand at 3250.990 kb on - strand, within PP_2847 at 3250.990 kb on - strand, within PP_2847 at 3251.229 kb on + strand, within PP_2848 at 3251.230 kb on - strand, within PP_2848 at 3251.362 kb on + strand, within PP_2848 at 3251.379 kb on + strand at 3251.813 kb on - strand at 3251.813 kb on - strand at 3251.813 kb on - strand at 3251.856 kb on + strand at 3251.857 kb on - strand at 3251.979 kb on + strand, within PP_2849 at 3251.979 kb on + strand, within PP_2849 at 3251.979 kb on + strand at 3251.980 kb on - strand, within PP_2849 at 3251.980 kb on - strand, within PP_2849 at 3252.318 kb on + strand, within PP_2849 at 3252.318 kb on + strand, within PP_2849 at 3252.318 kb on + strand, within PP_2849 at 3252.319 kb on - strand, within PP_2849 at 3252.319 kb on - strand, within PP_2849 at 3252.319 kb on - strand, within PP_2849 at 3252.319 kb on - strand, within PP_2849 at 3252.323 kb on - strand, within PP_2849 at 3252.562 kb on + strand at 3252.753 kb on + strand at 3252.829 kb on - strand, within PP_2851 at 3252.829 kb on - strand, within PP_2851
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida Missley MOI 0.1 remove 3,250,473 + PP_2846 0.75 -1.8 3,250,473 + PP_2846 0.75 +0.8 3,250,473 + PP_2846 0.75 -3.9 3,250,515 - PP_2846 0.84 -0.3 3,250,575 + -3.4 3,250,990 - PP_2847 0.68 +1.6 3,250,990 - PP_2847 0.68 +0.7 3,251,229 + PP_2848 0.10 -0.8 3,251,230 - PP_2848 0.10 -4.3 3,251,362 + PP_2848 0.30 -0.7 3,251,379 + +3.5 3,251,813 - -0.6 3,251,813 - -2.3 3,251,813 - -2.9 3,251,856 + -3.3 3,251,857 - -3.1 3,251,979 + PP_2849 0.21 -0.5 3,251,979 + PP_2849 0.21 +0.3 3,251,979 + +2.5 3,251,980 - PP_2849 0.21 +1.5 3,251,980 - PP_2849 0.21 -1.3 3,252,318 + PP_2849 0.75 -2.9 3,252,318 + PP_2849 0.75 -0.0 3,252,318 + PP_2849 0.75 -2.8 3,252,319 - PP_2849 0.76 +0.1 3,252,319 - PP_2849 0.76 +0.3 3,252,319 - PP_2849 0.76 -3.1 3,252,319 - PP_2849 0.76 +0.7 3,252,323 - PP_2849 0.76 -2.5 3,252,562 + -0.8 3,252,753 + +0.3 3,252,829 - PP_2851 0.16 -0.1 3,252,829 - PP_2851 0.16 +1.4
Or see this region's nucleotide sequence