Experiment: P. putida Missley MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2770 and PP_2771 are separated by 36 nucleotides PP_2771 and PP_2772 are separated by 8 nucleotides
PP_2770: PP_2770 - conserved exported protein of unknown function, at 3,153,797 to 3,155,122
_2770
PP_2771: PP_2771 - Sigma-54 dependent transcriptional regulator, at 3,155,159 to 3,156,262
_2771
PP_2772: PP_2772 - putative Monooxygenase, at 3,156,271 to 3,157,458
_2772
Position (kb)
3155
3156
3157 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3154.223 kb on + strand, within PP_2770 at 3154.224 kb on - strand, within PP_2770 at 3154.224 kb on - strand, within PP_2770 at 3154.261 kb on - strand, within PP_2770 at 3154.398 kb on - strand, within PP_2770 at 3154.398 kb on - strand, within PP_2770 at 3154.754 kb on + strand, within PP_2770 at 3154.757 kb on + strand, within PP_2770 at 3154.758 kb on - strand, within PP_2770 at 3154.758 kb on - strand, within PP_2770 at 3154.758 kb on - strand, within PP_2770 at 3154.935 kb on + strand, within PP_2770 at 3155.072 kb on + strand at 3155.072 kb on + strand at 3155.072 kb on + strand at 3155.090 kb on - strand at 3155.093 kb on - strand at 3155.168 kb on + strand at 3155.169 kb on - strand at 3155.169 kb on - strand at 3155.331 kb on - strand, within PP_2771 at 3155.569 kb on - strand, within PP_2771 at 3155.569 kb on - strand, within PP_2771 at 3155.607 kb on - strand, within PP_2771 at 3155.617 kb on - strand, within PP_2771 at 3155.695 kb on - strand, within PP_2771 at 3155.725 kb on - strand, within PP_2771 at 3155.727 kb on + strand, within PP_2771 at 3155.727 kb on + strand, within PP_2771 at 3155.728 kb on - strand, within PP_2771 at 3155.728 kb on - strand, within PP_2771 at 3156.077 kb on + strand, within PP_2771 at 3156.077 kb on + strand, within PP_2771 at 3156.077 kb on + strand, within PP_2771 at 3156.078 kb on - strand, within PP_2771 at 3156.272 kb on + strand at 3156.272 kb on + strand at 3156.279 kb on - strand at 3156.384 kb on - strand at 3156.621 kb on - strand, within PP_2772 at 3156.635 kb on + strand, within PP_2772 at 3156.636 kb on - strand, within PP_2772 at 3156.636 kb on - strand, within PP_2772 at 3156.636 kb on - strand, within PP_2772 at 3156.636 kb on - strand, within PP_2772 at 3156.733 kb on - strand, within PP_2772 at 3157.066 kb on + strand, within PP_2772 at 3157.067 kb on - strand, within PP_2772 at 3157.171 kb on + strand, within PP_2772
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida Missley MOI 0.1 remove 3,154,223 + PP_2770 0.32 -0.3 3,154,224 - PP_2770 0.32 +0.7 3,154,224 - PP_2770 0.32 -3.2 3,154,261 - PP_2770 0.35 -0.6 3,154,398 - PP_2770 0.45 +1.3 3,154,398 - PP_2770 0.45 -0.5 3,154,754 + PP_2770 0.72 +0.6 3,154,757 + PP_2770 0.72 -0.2 3,154,758 - PP_2770 0.72 -2.6 3,154,758 - PP_2770 0.72 -2.4 3,154,758 - PP_2770 0.72 -0.3 3,154,935 + PP_2770 0.86 +1.8 3,155,072 + -1.9 3,155,072 + +0.0 3,155,072 + -3.2 3,155,090 - -3.3 3,155,093 - -2.3 3,155,168 + +0.4 3,155,169 - -2.2 3,155,169 - +1.3 3,155,331 - PP_2771 0.16 -0.2 3,155,569 - PP_2771 0.37 -2.1 3,155,569 - PP_2771 0.37 -0.2 3,155,607 - PP_2771 0.41 -3.2 3,155,617 - PP_2771 0.41 -1.3 3,155,695 - PP_2771 0.49 -3.4 3,155,725 - PP_2771 0.51 -3.8 3,155,727 + PP_2771 0.51 -2.4 3,155,727 + PP_2771 0.51 -3.5 3,155,728 - PP_2771 0.52 +1.2 3,155,728 - PP_2771 0.52 -3.4 3,156,077 + PP_2771 0.83 -2.6 3,156,077 + PP_2771 0.83 -2.0 3,156,077 + PP_2771 0.83 -2.8 3,156,078 - PP_2771 0.83 -3.1 3,156,272 + -3.6 3,156,272 + -3.1 3,156,279 - -0.1 3,156,384 - -1.4 3,156,621 - PP_2772 0.29 -1.5 3,156,635 + PP_2772 0.31 +0.8 3,156,636 - PP_2772 0.31 -2.2 3,156,636 - PP_2772 0.31 +0.4 3,156,636 - PP_2772 0.31 +1.1 3,156,636 - PP_2772 0.31 -2.6 3,156,733 - PP_2772 0.39 -3.3 3,157,066 + PP_2772 0.67 +0.3 3,157,067 - PP_2772 0.67 -1.9 3,157,171 + PP_2772 0.76 +0.7
Or see this region's nucleotide sequence