Experiment: P. putida Missley MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2751 and PP_2752 are separated by 59 nucleotides PP_2752 and PP_2753 are separated by 116 nucleotides
PP_2751: PP_2751 - conserved exported protein of unknown function, at 3,133,948 to 3,135,288
_2751
PP_2752: PP_2752 - conserved exported protein of unknown function, at 3,135,348 to 3,136,682
_2752
PP_2753: PP_2753 - putative Branched-chain amino acid ABC transporter, ATP-binding protein, at 3,136,799 to 3,137,572
_2753
Position (kb)
3135
3136
3137 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3134.443 kb on + strand, within PP_2751 at 3134.444 kb on - strand, within PP_2751 at 3134.444 kb on - strand, within PP_2751 at 3134.444 kb on - strand, within PP_2751 at 3134.518 kb on + strand, within PP_2751 at 3134.563 kb on + strand, within PP_2751 at 3134.564 kb on - strand, within PP_2751 at 3134.697 kb on - strand, within PP_2751 at 3134.867 kb on + strand at 3134.867 kb on + strand, within PP_2751 at 3134.868 kb on - strand, within PP_2751 at 3134.868 kb on - strand, within PP_2751 at 3134.868 kb on - strand, within PP_2751 at 3134.920 kb on + strand, within PP_2751 at 3134.921 kb on - strand, within PP_2751 at 3134.921 kb on - strand, within PP_2751 at 3134.921 kb on - strand, within PP_2751 at 3135.286 kb on + strand at 3135.286 kb on + strand at 3135.286 kb on + strand at 3135.302 kb on - strand at 3135.371 kb on + strand at 3135.372 kb on - strand at 3135.372 kb on - strand at 3135.477 kb on + strand at 3135.519 kb on + strand, within PP_2752 at 3135.520 kb on - strand, within PP_2752 at 3135.529 kb on - strand, within PP_2752 at 3135.622 kb on - strand, within PP_2752 at 3135.694 kb on - strand, within PP_2752 at 3135.786 kb on + strand, within PP_2752 at 3135.840 kb on + strand, within PP_2752 at 3135.841 kb on - strand, within PP_2752 at 3135.961 kb on - strand, within PP_2752 at 3136.197 kb on + strand, within PP_2752 at 3136.317 kb on + strand, within PP_2752 at 3136.318 kb on - strand, within PP_2752 at 3136.320 kb on + strand, within PP_2752 at 3136.320 kb on + strand, within PP_2752 at 3136.320 kb on + strand, within PP_2752 at 3136.790 kb on + strand at 3136.790 kb on + strand at 3136.791 kb on - strand at 3136.794 kb on - strand at 3136.939 kb on - strand, within PP_2753 at 3137.210 kb on + strand, within PP_2753 at 3137.210 kb on + strand, within PP_2753 at 3137.211 kb on - strand, within PP_2753 at 3137.211 kb on - strand, within PP_2753 at 3137.474 kb on + strand, within PP_2753 at 3137.474 kb on + strand, within PP_2753 at 3137.475 kb on - strand, within PP_2753 at 3137.475 kb on - strand, within PP_2753 at 3137.475 kb on - strand, within PP_2753 at 3137.500 kb on - strand at 3137.540 kb on - strand at 3137.619 kb on + strand at 3137.620 kb on - strand at 3137.626 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida Missley MOI 0.1 remove 3,134,443 + PP_2751 0.37 +0.2 3,134,444 - PP_2751 0.37 -0.8 3,134,444 - PP_2751 0.37 -0.6 3,134,444 - PP_2751 0.37 +1.5 3,134,518 + PP_2751 0.43 +1.0 3,134,563 + PP_2751 0.46 +1.3 3,134,564 - PP_2751 0.46 +1.7 3,134,697 - PP_2751 0.56 +0.2 3,134,867 + -0.1 3,134,867 + PP_2751 0.69 -3.0 3,134,868 - PP_2751 0.69 -0.2 3,134,868 - PP_2751 0.69 -3.7 3,134,868 - PP_2751 0.69 -3.6 3,134,920 + PP_2751 0.72 +1.5 3,134,921 - PP_2751 0.73 +0.8 3,134,921 - PP_2751 0.73 -3.9 3,134,921 - PP_2751 0.73 +0.5 3,135,286 + -1.9 3,135,286 + +1.0 3,135,286 + +0.2 3,135,302 - -2.1 3,135,371 + -3.5 3,135,372 - -0.3 3,135,372 - -0.3 3,135,477 + -0.8 3,135,519 + PP_2752 0.13 +0.2 3,135,520 - PP_2752 0.13 -2.9 3,135,529 - PP_2752 0.14 -1.7 3,135,622 - PP_2752 0.21 -3.8 3,135,694 - PP_2752 0.26 -2.1 3,135,786 + PP_2752 0.33 +1.2 3,135,840 + PP_2752 0.37 +0.2 3,135,841 - PP_2752 0.37 -2.3 3,135,961 - PP_2752 0.46 +0.5 3,136,197 + PP_2752 0.64 -3.5 3,136,317 + PP_2752 0.73 -2.9 3,136,318 - PP_2752 0.73 -2.2 3,136,320 + PP_2752 0.73 -2.4 3,136,320 + PP_2752 0.73 -3.5 3,136,320 + PP_2752 0.73 -0.1 3,136,790 + +1.7 3,136,790 + -3.7 3,136,791 - -2.3 3,136,794 - -0.6 3,136,939 - PP_2753 0.18 -3.1 3,137,210 + PP_2753 0.53 -3.4 3,137,210 + PP_2753 0.53 -2.5 3,137,211 - PP_2753 0.53 -0.8 3,137,211 - PP_2753 0.53 +0.8 3,137,474 + PP_2753 0.87 -3.2 3,137,474 + PP_2753 0.87 -2.5 3,137,475 - PP_2753 0.87 -1.0 3,137,475 - PP_2753 0.87 +0.3 3,137,475 - PP_2753 0.87 -0.1 3,137,500 - +0.2 3,137,540 - -3.5 3,137,619 + -2.5 3,137,620 - +2.2 3,137,626 + +0.1
Or see this region's nucleotide sequence