Experiment: P. putida Missley MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1066 and PP_1067 overlap by 4 nucleotides PP_1067 and PP_1068 are separated by 65 nucleotides PP_1068 and PP_1069 overlap by 40 nucleotides
PP_1066: PP_1066 - C4-dicarboxylate transport transcriptional regulatory protein, at 1,219,977 to 1,221,305
_1066
PP_1067: PP_1067 - Sensor histidine kinase, at 1,221,302 to 1,223,209
_1067
PP_1068: PP_1068 - glutamate / aspartate ABC transporter - ATP binding subunit, at 1,223,275 to 1,224,045
_1068
PP_1069: PP_1069 - glutamate / aspartate ABC transporter - permease subunit, at 1,224,006 to 1,224,677
_1069
Position (kb)
1221
1222
1223
1224 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1220.392 kb on + strand, within PP_1066 at 1220.392 kb on + strand, within PP_1066 at 1220.392 kb on + strand, within PP_1066 at 1220.955 kb on + strand, within PP_1066 at 1220.956 kb on - strand, within PP_1066 at 1221.014 kb on + strand, within PP_1066 at 1221.201 kb on + strand at 1221.218 kb on + strand at 1221.287 kb on + strand at 1221.546 kb on + strand, within PP_1067 at 1221.546 kb on + strand, within PP_1067 at 1221.546 kb on + strand, within PP_1067 at 1221.547 kb on - strand, within PP_1067 at 1221.579 kb on - strand, within PP_1067 at 1221.749 kb on + strand, within PP_1067 at 1221.907 kb on + strand, within PP_1067 at 1221.907 kb on + strand, within PP_1067 at 1221.907 kb on + strand, within PP_1067 at 1221.908 kb on - strand, within PP_1067 at 1221.908 kb on - strand, within PP_1067 at 1221.994 kb on + strand, within PP_1067 at 1221.994 kb on + strand, within PP_1067 at 1221.994 kb on + strand, within PP_1067 at 1221.994 kb on + strand, within PP_1067 at 1222.341 kb on + strand, within PP_1067 at 1222.341 kb on + strand, within PP_1067 at 1222.342 kb on - strand, within PP_1067 at 1222.415 kb on + strand, within PP_1067 at 1222.416 kb on - strand, within PP_1067 at 1222.461 kb on + strand, within PP_1067 at 1222.461 kb on + strand, within PP_1067 at 1222.462 kb on - strand, within PP_1067 at 1222.464 kb on + strand, within PP_1067 at 1222.464 kb on + strand, within PP_1067 at 1222.464 kb on + strand, within PP_1067 at 1222.465 kb on - strand, within PP_1067 at 1222.465 kb on - strand, within PP_1067 at 1222.686 kb on + strand, within PP_1067 at 1222.687 kb on - strand, within PP_1067 at 1222.689 kb on + strand, within PP_1067 at 1222.690 kb on - strand, within PP_1067 at 1222.708 kb on + strand, within PP_1067 at 1222.722 kb on + strand, within PP_1067 at 1222.723 kb on - strand, within PP_1067 at 1222.827 kb on - strand, within PP_1067 at 1222.981 kb on - strand, within PP_1067 at 1222.981 kb on - strand, within PP_1067 at 1222.986 kb on + strand, within PP_1067 at 1222.987 kb on - strand, within PP_1067 at 1222.987 kb on - strand, within PP_1067 at 1222.987 kb on - strand, within PP_1067 at 1222.987 kb on - strand, within PP_1067 at 1222.987 kb on - strand, within PP_1067 at 1223.085 kb on + strand at 1223.085 kb on + strand at 1223.133 kb on - strand at 1223.221 kb on + strand at 1223.222 kb on - strand at 1223.435 kb on - strand, within PP_1068 at 1223.520 kb on + strand, within PP_1068 at 1223.521 kb on - strand, within PP_1068 at 1223.521 kb on - strand, within PP_1068 at 1223.639 kb on - strand, within PP_1068 at 1224.070 kb on + strand at 1224.166 kb on + strand, within PP_1069 at 1224.166 kb on + strand, within PP_1069 at 1224.167 kb on - strand, within PP_1069 at 1224.167 kb on - strand, within PP_1069 at 1224.168 kb on + strand, within PP_1069
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida Missley MOI 0.1 remove 1,220,392 + PP_1066 0.31 -1.2 1,220,392 + PP_1066 0.31 -1.7 1,220,392 + PP_1066 0.31 -2.8 1,220,955 + PP_1066 0.74 -1.7 1,220,956 - PP_1066 0.74 -2.3 1,221,014 + PP_1066 0.78 +0.7 1,221,201 + -2.4 1,221,218 + -2.7 1,221,287 + -2.3 1,221,546 + PP_1067 0.13 -1.0 1,221,546 + PP_1067 0.13 +1.5 1,221,546 + PP_1067 0.13 +0.5 1,221,547 - PP_1067 0.13 -1.4 1,221,579 - PP_1067 0.15 -2.4 1,221,749 + PP_1067 0.23 -2.9 1,221,907 + PP_1067 0.32 -0.4 1,221,907 + PP_1067 0.32 +0.2 1,221,907 + PP_1067 0.32 -0.5 1,221,908 - PP_1067 0.32 +1.8 1,221,908 - PP_1067 0.32 -2.3 1,221,994 + PP_1067 0.36 -3.1 1,221,994 + PP_1067 0.36 -2.3 1,221,994 + PP_1067 0.36 +0.5 1,221,994 + PP_1067 0.36 -1.8 1,222,341 + PP_1067 0.54 -2.6 1,222,341 + PP_1067 0.54 -1.9 1,222,342 - PP_1067 0.55 +1.6 1,222,415 + PP_1067 0.58 +0.3 1,222,416 - PP_1067 0.58 -3.5 1,222,461 + PP_1067 0.61 +0.9 1,222,461 + PP_1067 0.61 -0.2 1,222,462 - PP_1067 0.61 +0.7 1,222,464 + PP_1067 0.61 -2.6 1,222,464 + PP_1067 0.61 -2.3 1,222,464 + PP_1067 0.61 -3.1 1,222,465 - PP_1067 0.61 -2.9 1,222,465 - PP_1067 0.61 +1.9 1,222,686 + PP_1067 0.73 +2.2 1,222,687 - PP_1067 0.73 -2.5 1,222,689 + PP_1067 0.73 +0.1 1,222,690 - PP_1067 0.73 -3.4 1,222,708 + PP_1067 0.74 +0.7 1,222,722 + PP_1067 0.74 -0.4 1,222,723 - PP_1067 0.74 -1.8 1,222,827 - PP_1067 0.80 +2.4 1,222,981 - PP_1067 0.88 -2.4 1,222,981 - PP_1067 0.88 -3.1 1,222,986 + PP_1067 0.88 -2.6 1,222,987 - PP_1067 0.88 -4.6 1,222,987 - PP_1067 0.88 +1.2 1,222,987 - PP_1067 0.88 -2.4 1,222,987 - PP_1067 0.88 -2.4 1,222,987 - PP_1067 0.88 -2.9 1,223,085 + +1.5 1,223,085 + -2.5 1,223,133 - +0.7 1,223,221 + -1.4 1,223,222 - +0.1 1,223,435 - PP_1068 0.21 +0.1 1,223,520 + PP_1068 0.32 -1.9 1,223,521 - PP_1068 0.32 +0.7 1,223,521 - PP_1068 0.32 -1.1 1,223,639 - PP_1068 0.47 -0.1 1,224,070 + +0.6 1,224,166 + PP_1069 0.24 +0.1 1,224,166 + PP_1069 0.24 -1.1 1,224,167 - PP_1069 0.24 -0.3 1,224,167 - PP_1069 0.24 -1.1 1,224,168 + PP_1069 0.24 +0.9
Or see this region's nucleotide sequence