Experiment: P. putida Missley MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0156 and PP_0157 are separated by 419 nucleotides PP_0157 and PP_0158 are separated by 160 nucleotides
PP_0156: PP_0156 - pyridine nucleotide transhydrogenase subunit alpha, at 165,785 to 166,906
_0156
PP_0157: PP_0157 - transcriptional activator of gcdH, LysR family, at 167,326 to 168,243
_0157
PP_0158: PP_0158 - glutaryl-CoA dehydrogenase, at 168,404 to 169,585
_0158
Position (kb) 167
168
169 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 166.400 kb on + strand, within PP_0156 at 166.464 kb on + strand, within PP_0156 at 166.464 kb on + strand, within PP_0156 at 166.465 kb on - strand, within PP_0156 at 166.465 kb on - strand, within PP_0156 at 166.465 kb on - strand, within PP_0156 at 166.494 kb on + strand, within PP_0156 at 166.494 kb on + strand, within PP_0156 at 166.495 kb on - strand, within PP_0156 at 166.495 kb on - strand, within PP_0156 at 166.580 kb on - strand, within PP_0156 at 166.700 kb on + strand, within PP_0156 at 166.716 kb on + strand, within PP_0156 at 166.716 kb on + strand, within PP_0156 at 166.910 kb on + strand at 166.910 kb on + strand at 166.910 kb on + strand at 166.910 kb on + strand at 166.910 kb on + strand at 166.911 kb on - strand at 166.911 kb on - strand at 166.911 kb on - strand at 166.940 kb on + strand at 166.941 kb on - strand at 166.941 kb on - strand at 167.076 kb on + strand at 167.076 kb on + strand at 167.185 kb on - strand at 167.191 kb on - strand at 167.208 kb on + strand at 167.225 kb on + strand at 167.239 kb on - strand at 167.285 kb on - strand at 167.285 kb on - strand at 167.285 kb on - strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.287 kb on - strand at 167.287 kb on - strand at 167.287 kb on - strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.298 kb on + strand at 167.307 kb on - strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.353 kb on - strand at 167.353 kb on - strand at 167.353 kb on - strand at 167.353 kb on - strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.360 kb on + strand at 167.360 kb on + strand at 167.361 kb on - strand at 167.361 kb on - strand at 167.378 kb on - strand at 167.441 kb on + strand, within PP_0157 at 167.441 kb on + strand, within PP_0157 at 167.442 kb on - strand, within PP_0157 at 167.442 kb on - strand, within PP_0157 at 167.442 kb on - strand, within PP_0157 at 167.445 kb on - strand, within PP_0157 at 167.445 kb on - strand, within PP_0157 at 167.445 kb on - strand, within PP_0157 at 167.458 kb on - strand, within PP_0157 at 167.476 kb on - strand, within PP_0157 at 167.527 kb on + strand, within PP_0157 at 167.528 kb on - strand, within PP_0157 at 167.578 kb on + strand, within PP_0157 at 167.578 kb on + strand, within PP_0157 at 167.578 kb on + strand, within PP_0157 at 167.579 kb on - strand, within PP_0157 at 167.579 kb on - strand, within PP_0157 at 167.585 kb on + strand, within PP_0157 at 167.586 kb on - strand, within PP_0157 at 167.807 kb on + strand, within PP_0157 at 167.808 kb on - strand, within PP_0157 at 167.808 kb on - strand, within PP_0157 at 167.827 kb on - strand, within PP_0157 at 167.827 kb on - strand, within PP_0157 at 167.914 kb on + strand, within PP_0157 at 167.915 kb on - strand, within PP_0157 at 167.994 kb on + strand, within PP_0157 at 167.994 kb on + strand, within PP_0157 at 167.995 kb on - strand, within PP_0157 at 168.039 kb on - strand, within PP_0157 at 168.099 kb on + strand, within PP_0157 at 168.174 kb on + strand at 168.245 kb on + strand at 168.262 kb on + strand at 168.263 kb on - strand at 168.263 kb on - strand at 168.263 kb on - strand at 168.263 kb on - strand at 168.274 kb on + strand at 168.275 kb on - strand at 168.275 kb on - strand at 168.358 kb on - strand at 168.386 kb on + strand at 168.386 kb on + strand at 168.621 kb on - strand, within PP_0158 at 168.641 kb on + strand, within PP_0158 at 168.641 kb on + strand, within PP_0158 at 168.641 kb on + strand, within PP_0158 at 168.641 kb on + strand, within PP_0158 at 168.641 kb on + strand, within PP_0158 at 168.641 kb on + strand, within PP_0158 at 168.642 kb on - strand, within PP_0158 at 168.642 kb on - strand, within PP_0158 at 168.642 kb on - strand, within PP_0158 at 168.642 kb on - strand, within PP_0158 at 168.642 kb on - strand, within PP_0158 at 168.650 kb on + strand, within PP_0158 at 168.650 kb on + strand, within PP_0158 at 168.650 kb on + strand, within PP_0158 at 168.651 kb on - strand, within PP_0158 at 168.658 kb on + strand, within PP_0158 at 168.773 kb on + strand, within PP_0158 at 168.829 kb on + strand, within PP_0158 at 168.830 kb on - strand, within PP_0158 at 169.160 kb on + strand, within PP_0158 at 169.160 kb on + strand, within PP_0158 at 169.160 kb on + strand, within PP_0158 at 169.160 kb on + strand, within PP_0158 at 169.161 kb on - strand, within PP_0158 at 169.165 kb on + strand, within PP_0158 at 169.220 kb on + strand, within PP_0158 at 169.220 kb on + strand, within PP_0158 at 169.220 kb on + strand, within PP_0158 at 169.221 kb on - strand, within PP_0158
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida Missley MOI 0.1 remove 166,400 + PP_0156 0.55 +0.1 166,464 + PP_0156 0.61 +0.5 166,464 + PP_0156 0.61 +0.6 166,465 - PP_0156 0.61 +1.6 166,465 - PP_0156 0.61 -0.9 166,465 - PP_0156 0.61 +1.4 166,494 + PP_0156 0.63 +1.1 166,494 + PP_0156 0.63 +1.2 166,495 - PP_0156 0.63 +1.7 166,495 - PP_0156 0.63 -0.5 166,580 - PP_0156 0.71 +1.6 166,700 + PP_0156 0.82 -3.0 166,716 + PP_0156 0.83 +0.0 166,716 + PP_0156 0.83 +2.0 166,910 + +1.7 166,910 + +0.4 166,910 + +1.7 166,910 + -0.1 166,910 + +2.2 166,911 - +1.5 166,911 - +0.6 166,911 - +0.4 166,940 + +0.4 166,941 - -0.6 166,941 - -1.6 167,076 + +2.3 167,076 + +0.5 167,185 - +0.1 167,191 - +1.2 167,208 + -0.2 167,225 + +0.5 167,239 - +2.5 167,285 - +2.4 167,285 - +1.0 167,285 - +1.0 167,286 + +1.8 167,286 + -0.3 167,286 + +1.2 167,286 + -0.2 167,286 + +1.0 167,286 + -2.0 167,286 + +0.4 167,286 + -0.1 167,286 + +2.1 167,287 - +0.8 167,287 - +0.4 167,287 - +0.8 167,290 + +1.9 167,290 + +0.3 167,290 + -0.2 167,290 + +0.7 167,290 + +0.7 167,290 + -3.0 167,290 + +1.9 167,290 + +2.2 167,290 + +1.7 167,290 + +1.2 167,290 + +0.2 167,290 + +1.8 167,290 + +0.6 167,290 + +1.5 167,290 + -1.2 167,290 + +0.7 167,291 - -1.3 167,291 - +2.0 167,291 - -0.6 167,291 - +0.8 167,291 - +1.9 167,291 - -0.3 167,291 - -1.4 167,291 - +0.5 167,291 - +0.8 167,291 - -0.0 167,298 + +1.6 167,307 - -2.7 167,352 + +2.9 167,352 + +0.7 167,352 + +1.6 167,352 + +1.7 167,352 + -0.0 167,353 - +1.1 167,353 - +0.4 167,353 - +1.0 167,353 - +1.1 167,356 + +1.2 167,356 + +0.7 167,356 + +1.0 167,356 + +0.9 167,356 + +0.5 167,356 + +0.4 167,357 - +0.8 167,357 - -2.5 167,357 - +0.1 167,357 - -0.6 167,357 - +2.0 167,357 - +1.3 167,360 + -2.2 167,360 + +1.2 167,361 - +2.2 167,361 - +0.5 167,378 - +1.7 167,441 + PP_0157 0.13 +2.3 167,441 + PP_0157 0.13 +2.4 167,442 - PP_0157 0.13 +1.7 167,442 - PP_0157 0.13 +2.5 167,442 - PP_0157 0.13 +1.7 167,445 - PP_0157 0.13 +1.8 167,445 - PP_0157 0.13 +0.4 167,445 - PP_0157 0.13 +2.0 167,458 - PP_0157 0.14 +2.2 167,476 - PP_0157 0.16 +1.2 167,527 + PP_0157 0.22 +0.7 167,528 - PP_0157 0.22 +2.2 167,578 + PP_0157 0.27 +0.7 167,578 + PP_0157 0.27 +1.0 167,578 + PP_0157 0.27 +1.3 167,579 - PP_0157 0.28 -0.4 167,579 - PP_0157 0.28 +0.5 167,585 + PP_0157 0.28 -0.8 167,586 - PP_0157 0.28 +1.4 167,807 + PP_0157 0.52 +0.6 167,808 - PP_0157 0.53 +0.7 167,808 - PP_0157 0.53 -0.1 167,827 - PP_0157 0.55 +0.7 167,827 - PP_0157 0.55 +2.1 167,914 + PP_0157 0.64 +1.0 167,915 - PP_0157 0.64 +1.3 167,994 + PP_0157 0.73 +0.9 167,994 + PP_0157 0.73 -0.6 167,995 - PP_0157 0.73 +0.1 168,039 - PP_0157 0.78 +1.0 168,099 + PP_0157 0.84 -3.5 168,174 + +0.4 168,245 + -0.0 168,262 + +1.9 168,263 - +1.9 168,263 - +1.6 168,263 - -2.7 168,263 - +1.2 168,274 + +0.7 168,275 - +0.9 168,275 - +1.4 168,358 - +2.0 168,386 + +1.3 168,386 + +1.2 168,621 - PP_0158 0.18 +0.9 168,641 + PP_0158 0.20 -1.5 168,641 + PP_0158 0.20 +0.5 168,641 + PP_0158 0.20 +1.7 168,641 + PP_0158 0.20 +1.7 168,641 + PP_0158 0.20 -0.8 168,641 + PP_0158 0.20 +1.8 168,642 - PP_0158 0.20 +1.6 168,642 - PP_0158 0.20 -0.9 168,642 - PP_0158 0.20 +2.4 168,642 - PP_0158 0.20 +2.6 168,642 - PP_0158 0.20 +2.0 168,650 + PP_0158 0.21 -0.1 168,650 + PP_0158 0.21 -0.1 168,650 + PP_0158 0.21 -3.2 168,651 - PP_0158 0.21 +1.4 168,658 + PP_0158 0.21 +1.4 168,773 + PP_0158 0.31 -1.1 168,829 + PP_0158 0.36 +1.7 168,830 - PP_0158 0.36 +0.2 169,160 + PP_0158 0.64 +1.2 169,160 + PP_0158 0.64 -4.1 169,160 + PP_0158 0.64 -0.5 169,160 + PP_0158 0.64 +1.9 169,161 - PP_0158 0.64 +1.9 169,165 + PP_0158 0.64 +2.2 169,220 + PP_0158 0.69 -0.6 169,220 + PP_0158 0.69 +1.5 169,220 + PP_0158 0.69 +0.8 169,221 - PP_0158 0.69 +1.4
Or see this region's nucleotide sequence