Experiment: carbon source D-Glucose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gldA and fsaB are separated by 10 nucleotides fsaB and ptsA are separated by 11 nucleotides ptsA and frwC are separated by 308 nucleotides
b3945: gldA - glycerol dehydrogenase, (NAD) (VIMSS), at 4,135,955 to 4,137,058
gldA
b3946: fsaB - fructose-6-phosphate aldolase 2 (NCBI), at 4,137,069 to 4,137,731
fsaB
b3947: ptsA - fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component (NCBI), at 4,137,743 to 4,140,244
ptsA
b3949: frwC - predicted enzyme IIC component of PTS (NCBI), at 4,140,553 to 4,141,632
frwC
Position (kb)
4137
4138
4139
4140
4141 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4136.784 kb on + strand, within gldA at 4136.784 kb on + strand, within gldA at 4136.812 kb on - strand, within gldA at 4136.812 kb on - strand, within gldA at 4136.916 kb on + strand at 4136.970 kb on + strand at 4136.970 kb on + strand at 4137.030 kb on - strand at 4137.061 kb on - strand at 4137.061 kb on - strand at 4137.105 kb on - strand at 4137.105 kb on - strand at 4137.105 kb on - strand at 4137.196 kb on + strand, within fsaB at 4137.214 kb on + strand, within fsaB at 4137.215 kb on - strand, within fsaB at 4137.234 kb on - strand, within fsaB at 4137.288 kb on + strand, within fsaB at 4137.288 kb on + strand, within fsaB at 4137.288 kb on + strand, within fsaB at 4137.291 kb on - strand, within fsaB at 4137.296 kb on - strand, within fsaB at 4137.296 kb on - strand, within fsaB at 4137.344 kb on - strand, within fsaB at 4137.389 kb on - strand, within fsaB at 4137.389 kb on - strand, within fsaB at 4137.393 kb on + strand, within fsaB at 4137.396 kb on + strand, within fsaB at 4137.401 kb on - strand, within fsaB at 4137.401 kb on - strand, within fsaB at 4137.404 kb on - strand, within fsaB at 4137.404 kb on - strand, within fsaB at 4137.404 kb on - strand, within fsaB at 4137.404 kb on - strand, within fsaB at 4137.468 kb on - strand, within fsaB at 4137.507 kb on + strand, within fsaB at 4137.525 kb on - strand, within fsaB at 4137.525 kb on - strand, within fsaB at 4137.539 kb on - strand, within fsaB at 4137.539 kb on - strand, within fsaB at 4137.552 kb on + strand, within fsaB at 4137.610 kb on + strand, within fsaB at 4137.647 kb on + strand, within fsaB at 4137.703 kb on + strand at 4137.703 kb on - strand at 4137.705 kb on - strand at 4137.866 kb on - strand at 4137.866 kb on - strand at 4137.900 kb on - strand at 4137.900 kb on - strand at 4137.900 kb on - strand at 4138.103 kb on - strand, within ptsA at 4138.103 kb on - strand, within ptsA at 4138.112 kb on - strand, within ptsA at 4138.124 kb on - strand, within ptsA at 4138.205 kb on - strand, within ptsA at 4138.205 kb on - strand, within ptsA at 4138.208 kb on - strand, within ptsA at 4138.259 kb on + strand, within ptsA at 4138.351 kb on + strand, within ptsA at 4138.396 kb on + strand, within ptsA at 4138.398 kb on + strand, within ptsA at 4138.398 kb on + strand, within ptsA at 4138.444 kb on - strand, within ptsA at 4138.444 kb on - strand, within ptsA at 4138.446 kb on + strand, within ptsA at 4138.459 kb on + strand, within ptsA at 4138.467 kb on - strand, within ptsA at 4138.662 kb on - strand, within ptsA at 4138.705 kb on + strand, within ptsA at 4138.705 kb on + strand, within ptsA at 4138.752 kb on + strand, within ptsA at 4138.787 kb on + strand, within ptsA at 4138.787 kb on + strand, within ptsA at 4138.792 kb on + strand, within ptsA at 4138.797 kb on + strand, within ptsA at 4138.823 kb on + strand, within ptsA at 4138.926 kb on - strand, within ptsA at 4138.930 kb on + strand, within ptsA at 4139.012 kb on - strand, within ptsA at 4139.032 kb on - strand, within ptsA at 4139.032 kb on - strand, within ptsA at 4139.070 kb on + strand, within ptsA at 4139.070 kb on + strand, within ptsA at 4139.078 kb on - strand, within ptsA at 4139.128 kb on - strand, within ptsA at 4139.148 kb on + strand, within ptsA at 4139.152 kb on + strand, within ptsA at 4139.152 kb on + strand, within ptsA at 4139.249 kb on + strand, within ptsA at 4139.249 kb on + strand, within ptsA at 4139.303 kb on - strand, within ptsA at 4139.422 kb on + strand, within ptsA at 4139.438 kb on - strand, within ptsA at 4139.709 kb on - strand, within ptsA at 4139.770 kb on - strand, within ptsA at 4139.782 kb on - strand, within ptsA at 4139.782 kb on - strand, within ptsA at 4139.787 kb on - strand, within ptsA at 4139.878 kb on + strand, within ptsA at 4139.888 kb on - strand, within ptsA at 4139.891 kb on + strand, within ptsA at 4140.001 kb on + strand at 4140.001 kb on + strand at 4140.009 kb on + strand at 4140.096 kb on - strand at 4140.130 kb on + strand at 4140.133 kb on - strand at 4140.133 kb on - strand at 4140.196 kb on + strand at 4140.196 kb on + strand at 4140.204 kb on - strand at 4140.206 kb on + strand at 4140.206 kb on + strand at 4140.285 kb on - strand at 4140.295 kb on + strand at 4140.539 kb on + strand at 4140.667 kb on + strand, within frwC at 4140.667 kb on + strand, within frwC at 4140.685 kb on + strand, within frwC at 4140.685 kb on + strand, within frwC at 4140.685 kb on + strand, within frwC at 4140.693 kb on - strand, within frwC at 4140.756 kb on + strand, within frwC at 4140.805 kb on + strand, within frwC at 4140.808 kb on - strand, within frwC at 4140.827 kb on + strand, within frwC at 4140.827 kb on + strand, within frwC at 4140.830 kb on + strand, within frwC at 4140.895 kb on + strand, within frwC at 4140.895 kb on + strand, within frwC at 4140.951 kb on + strand, within frwC at 4141.002 kb on - strand, within frwC at 4141.010 kb on + strand, within frwC at 4141.010 kb on + strand, within frwC at 4141.051 kb on - strand, within frwC at 4141.066 kb on + strand, within frwC at 4141.069 kb on + strand, within frwC at 4141.069 kb on + strand, within frwC at 4141.069 kb on + strand, within frwC at 4141.092 kb on - strand, within frwC at 4141.105 kb on + strand, within frwC at 4141.130 kb on - strand, within frwC at 4141.133 kb on + strand, within frwC at 4141.154 kb on + strand, within frwC at 4141.167 kb on + strand, within frwC at 4141.191 kb on - strand, within frwC at 4141.194 kb on - strand, within frwC at 4141.194 kb on - strand, within frwC at 4141.218 kb on + strand, within frwC at 4141.218 kb on + strand, within frwC
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.25mM remove 4,136,784 + gldA b3945 0.75 +1.9 4,136,784 + gldA b3945 0.75 +0.9 4,136,812 - gldA b3945 0.78 -2.6 4,136,812 - gldA b3945 0.78 -2.0 4,136,916 + -1.0 4,136,970 + +1.4 4,136,970 + -4.0 4,137,030 - +1.1 4,137,061 - -0.5 4,137,061 - -2.8 4,137,105 - +0.8 4,137,105 - -2.7 4,137,105 - -3.8 4,137,196 + fsaB b3946 0.19 +0.7 4,137,214 + fsaB b3946 0.22 -0.5 4,137,215 - fsaB b3946 0.22 -3.4 4,137,234 - fsaB b3946 0.25 +0.5 4,137,288 + fsaB b3946 0.33 +1.3 4,137,288 + fsaB b3946 0.33 -3.8 4,137,288 + fsaB b3946 0.33 -3.9 4,137,291 - fsaB b3946 0.33 -3.6 4,137,296 - fsaB b3946 0.34 -3.5 4,137,296 - fsaB b3946 0.34 +2.1 4,137,344 - fsaB b3946 0.41 +0.1 4,137,389 - fsaB b3946 0.48 -2.8 4,137,389 - fsaB b3946 0.48 -2.9 4,137,393 + fsaB b3946 0.49 +0.4 4,137,396 + fsaB b3946 0.49 -0.3 4,137,401 - fsaB b3946 0.50 +0.7 4,137,401 - fsaB b3946 0.50 +1.7 4,137,404 - fsaB b3946 0.51 +0.4 4,137,404 - fsaB b3946 0.51 +1.9 4,137,404 - fsaB b3946 0.51 -0.0 4,137,404 - fsaB b3946 0.51 +1.1 4,137,468 - fsaB b3946 0.60 -3.8 4,137,507 + fsaB b3946 0.66 +0.6 4,137,525 - fsaB b3946 0.69 +0.2 4,137,525 - fsaB b3946 0.69 -3.1 4,137,539 - fsaB b3946 0.71 -0.3 4,137,539 - fsaB b3946 0.71 +0.7 4,137,552 + fsaB b3946 0.73 -4.1 4,137,610 + fsaB b3946 0.82 +1.6 4,137,647 + fsaB b3946 0.87 -0.4 4,137,703 + -0.8 4,137,703 - +0.3 4,137,705 - -0.2 4,137,866 - +0.3 4,137,866 - -3.6 4,137,900 - -2.7 4,137,900 - -0.9 4,137,900 - -2.9 4,138,103 - ptsA b3947 0.14 -0.5 4,138,103 - ptsA b3947 0.14 -3.6 4,138,112 - ptsA b3947 0.15 +1.6 4,138,124 - ptsA b3947 0.15 -0.4 4,138,205 - ptsA b3947 0.18 -2.6 4,138,205 - ptsA b3947 0.18 -2.5 4,138,208 - ptsA b3947 0.19 -2.0 4,138,259 + ptsA b3947 0.21 -1.7 4,138,351 + ptsA b3947 0.24 -0.7 4,138,396 + ptsA b3947 0.26 +0.5 4,138,398 + ptsA b3947 0.26 -3.9 4,138,398 + ptsA b3947 0.26 +0.1 4,138,444 - ptsA b3947 0.28 +1.4 4,138,444 - ptsA b3947 0.28 +0.1 4,138,446 + ptsA b3947 0.28 -0.9 4,138,459 + ptsA b3947 0.29 +1.7 4,138,467 - ptsA b3947 0.29 -2.9 4,138,662 - ptsA b3947 0.37 +0.3 4,138,705 + ptsA b3947 0.38 -0.3 4,138,705 + ptsA b3947 0.38 +0.4 4,138,752 + ptsA b3947 0.40 +0.7 4,138,787 + ptsA b3947 0.42 +0.3 4,138,787 + ptsA b3947 0.42 +0.2 4,138,792 + ptsA b3947 0.42 -4.0 4,138,797 + ptsA b3947 0.42 -5.0 4,138,823 + ptsA b3947 0.43 +0.5 4,138,926 - ptsA b3947 0.47 -1.8 4,138,930 + ptsA b3947 0.47 -5.4 4,139,012 - ptsA b3947 0.51 -0.8 4,139,032 - ptsA b3947 0.52 -2.6 4,139,032 - ptsA b3947 0.52 -0.5 4,139,070 + ptsA b3947 0.53 -2.2 4,139,070 + ptsA b3947 0.53 +1.3 4,139,078 - ptsA b3947 0.53 -0.9 4,139,128 - ptsA b3947 0.55 -1.7 4,139,148 + ptsA b3947 0.56 -0.5 4,139,152 + ptsA b3947 0.56 +0.4 4,139,152 + ptsA b3947 0.56 -0.6 4,139,249 + ptsA b3947 0.60 +1.8 4,139,249 + ptsA b3947 0.60 -1.0 4,139,303 - ptsA b3947 0.62 -0.3 4,139,422 + ptsA b3947 0.67 -2.3 4,139,438 - ptsA b3947 0.68 -0.4 4,139,709 - ptsA b3947 0.79 +1.0 4,139,770 - ptsA b3947 0.81 +1.7 4,139,782 - ptsA b3947 0.81 +1.1 4,139,782 - ptsA b3947 0.81 -2.9 4,139,787 - ptsA b3947 0.82 -2.6 4,139,878 + ptsA b3947 0.85 +0.8 4,139,888 - ptsA b3947 0.86 +0.2 4,139,891 + ptsA b3947 0.86 +2.0 4,140,001 + -0.5 4,140,001 + -3.8 4,140,009 + +0.2 4,140,096 - -2.7 4,140,130 + +0.7 4,140,133 - -0.0 4,140,133 - +2.5 4,140,196 + -2.7 4,140,196 + -2.1 4,140,204 - +1.2 4,140,206 + -2.7 4,140,206 + +0.8 4,140,285 - -0.7 4,140,295 + -0.6 4,140,539 + +0.9 4,140,667 + frwC b3949 0.11 +1.0 4,140,667 + frwC b3949 0.11 +1.4 4,140,685 + frwC b3949 0.12 -2.8 4,140,685 + frwC b3949 0.12 -0.4 4,140,685 + frwC b3949 0.12 -4.2 4,140,693 - frwC b3949 0.13 +1.1 4,140,756 + frwC b3949 0.19 -0.4 4,140,805 + frwC b3949 0.23 -1.1 4,140,808 - frwC b3949 0.24 +0.8 4,140,827 + frwC b3949 0.25 +0.4 4,140,827 + frwC b3949 0.25 +1.8 4,140,830 + frwC b3949 0.26 +0.5 4,140,895 + frwC b3949 0.32 -2.6 4,140,895 + frwC b3949 0.32 +1.4 4,140,951 + frwC b3949 0.37 -0.9 4,141,002 - frwC b3949 0.42 +0.7 4,141,010 + frwC b3949 0.42 -3.6 4,141,010 + frwC b3949 0.42 +1.2 4,141,051 - frwC b3949 0.46 +1.7 4,141,066 + frwC b3949 0.47 -3.4 4,141,069 + frwC b3949 0.48 -3.2 4,141,069 + frwC b3949 0.48 -3.2 4,141,069 + frwC b3949 0.48 -0.9 4,141,092 - frwC b3949 0.50 +0.3 4,141,105 + frwC b3949 0.51 +1.3 4,141,130 - frwC b3949 0.53 +0.6 4,141,133 + frwC b3949 0.54 +0.3 4,141,154 + frwC b3949 0.56 +0.8 4,141,167 + frwC b3949 0.57 +0.9 4,141,191 - frwC b3949 0.59 -3.7 4,141,194 - frwC b3949 0.59 +0.4 4,141,194 - frwC b3949 0.59 -3.3 4,141,218 + frwC b3949 0.62 -0.2 4,141,218 + frwC b3949 0.62 -1.9
Or see this region's nucleotide sequence