Experiment: carbon source D-Glucose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yecA and leuZ are separated by 195 nucleotides leuZ and cysT are separated by 12 nucleotides cysT and glyW are separated by 54 nucleotides glyW and pgsA are separated by 151 nucleotides pgsA and uvrC are separated by 56 nucleotides
b1908: yecA - conserved metal-binding protein (NCBI), at 1,988,978 to 1,989,643
yecA
b1909: leuZ - tRNA-Leu (NCBI), at 1,989,839 to 1,989,925
leuZ
b1910: cysT - tRNA-Cys (NCBI), at 1,989,938 to 1,990,011
cysT
b1911: glyW - tRNA-Gly (NCBI), at 1,990,066 to 1,990,141
glyW
b1912: pgsA - phosphatidylglycerophosphate synthetase (NCBI), at 1,990,293 to 1,990,841
pgsA
b1913: uvrC - excinuclease ABC, subunit C; repair of UV damage to DNA (VIMSS), at 1,990,898 to 1,992,730
uvrC
Position (kb)
1989
1990
1991 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1988.957 kb on - strand at 1989.096 kb on + strand, within yecA at 1989.154 kb on - strand, within yecA at 1989.198 kb on + strand, within yecA at 1989.216 kb on - strand, within yecA at 1989.216 kb on - strand, within yecA at 1989.265 kb on + strand, within yecA at 1989.348 kb on + strand, within yecA at 1989.348 kb on + strand, within yecA at 1989.454 kb on + strand, within yecA at 1990.916 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.25mM remove 1,988,957 - +0.7 1,989,096 + yecA b1908 0.18 -2.5 1,989,154 - yecA b1908 0.26 -0.5 1,989,198 + yecA b1908 0.33 +1.4 1,989,216 - yecA b1908 0.36 -0.5 1,989,216 - yecA b1908 0.36 -3.6 1,989,265 + yecA b1908 0.43 -2.5 1,989,348 + yecA b1908 0.56 -2.8 1,989,348 + yecA b1908 0.56 -2.9 1,989,454 + yecA b1908 0.71 -2.4 1,990,916 + +1.3
Or see this region's nucleotide sequence