Strain Fitness in Escherichia coli BW25113 around b3008

Experiment: carbon source D-Glucose 0.25mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntexbD and exbB are separated by 6 nucleotidesexbB and metC are separated by 251 nucleotidesmetC and yghB are separated by 139 nucleotidesyghB and yqhC are separated by 39 nucleotides b3005: exbD - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 3,148,840 to 3,149,265 exbD b3006: exbB - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 3,149,272 to 3,150,006 exbB b3008: metC - cystathionine beta-lyase (NCBI), at 3,150,258 to 3,151,445 metC b3009: yghB - conserved inner membrane protein (NCBI), at 3,151,585 to 3,152,244 yghB b3010: yqhC - putative ARAC-type regulatory protein (VIMSS), at 3,152,284 to 3,153,240 yqhC Position (kb) 3150 3151 3152Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 3149.377 kb on + strand, within exbBat 3149.377 kb on + strand, within exbBat 3149.380 kb on + strand, within exbBat 3149.396 kb on - strand, within exbBat 3149.448 kb on - strand, within exbBat 3149.501 kb on + strand, within exbBat 3149.508 kb on + strand, within exbBat 3149.543 kb on - strand, within exbBat 3149.553 kb on - strand, within exbBat 3149.554 kb on + strand, within exbBat 3149.564 kb on + strand, within exbBat 3149.659 kb on + strand, within exbBat 3149.682 kb on - strand, within exbBat 3149.682 kb on - strand, within exbBat 3149.690 kb on - strand, within exbBat 3149.707 kb on + strand, within exbBat 3149.710 kb on - strand, within exbBat 3149.718 kb on + strand, within exbBat 3149.803 kb on - strand, within exbBat 3149.803 kb on - strand, within exbBat 3149.866 kb on + strand, within exbBat 3149.956 kb on + strandat 3149.956 kb on + strandat 3149.956 kb on + strandat 3149.983 kb on + strandat 3150.016 kb on - strandat 3150.257 kb on - strandat 3150.288 kb on - strandat 3150.292 kb on + strandat 3150.300 kb on - strandat 3150.300 kb on - strandat 3150.337 kb on - strandat 3150.378 kb on - strand, within metCat 3150.421 kb on + strand, within metCat 3150.421 kb on + strand, within metCat 3150.514 kb on - strand, within metCat 3150.575 kb on + strand, within metCat 3150.577 kb on - strand, within metCat 3150.583 kb on - strand, within metCat 3150.640 kb on - strand, within metCat 3150.654 kb on + strand, within metCat 3150.669 kb on + strand, within metCat 3150.706 kb on - strand, within metCat 3150.791 kb on - strand, within metCat 3150.820 kb on + strand, within metCat 3150.820 kb on + strand, within metCat 3150.912 kb on + strand, within metCat 3150.914 kb on + strand, within metCat 3150.915 kb on + strand, within metCat 3150.932 kb on - strand, within metCat 3151.015 kb on + strandat 3151.015 kb on + strand, within metCat 3151.028 kb on + strand, within metCat 3151.038 kb on - strand, within metCat 3151.038 kb on - strand, within metCat 3151.074 kb on - strand, within metCat 3151.074 kb on - strand, within metCat 3151.120 kb on + strand, within metCat 3151.125 kb on + strand, within metCat 3151.138 kb on - strand, within metCat 3151.216 kb on + strand, within metCat 3151.216 kb on + strand, within metCat 3151.237 kb on + strand, within metCat 3151.274 kb on - strand, within metCat 3151.376 kb on + strandat 3151.655 kb on - strand, within yghBat 3151.859 kb on + strand, within yghBat 3151.859 kb on + strand, within yghBat 3151.867 kb on - strand, within yghBat 3151.889 kb on + strand, within yghBat 3151.889 kb on + strand, within yghBat 3152.046 kb on + strand, within yghBat 3152.046 kb on + strand, within yghBat 3152.076 kb on + strand, within yghBat 3152.084 kb on - strand, within yghBat 3152.101 kb on - strand, within yghBat 3152.101 kb on - strand, within yghBat 3152.130 kb on + strand, within yghBat 3152.133 kb on + strand, within yghBat 3152.152 kb on - strand, within yghBat 3152.153 kb on - strand, within yghBat 3152.186 kb on + strandat 3152.256 kb on + strandat 3152.256 kb on + strandat 3152.280 kb on + strandat 3152.290 kb on + strandat 3152.290 kb on + strandat 3152.372 kb on + strandat 3152.377 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.25mM
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3,149,377 + exbB b3006 0.14 +1.1
3,149,377 + exbB b3006 0.14 +0.2
3,149,380 + exbB b3006 0.15 +1.4
3,149,396 - exbB b3006 0.17 +1.3
3,149,448 - exbB b3006 0.24 -3.4
3,149,501 + exbB b3006 0.31 +1.9
3,149,508 + exbB b3006 0.32 -1.9
3,149,543 - exbB b3006 0.37 +1.1
3,149,553 - exbB b3006 0.38 -2.2
3,149,554 + exbB b3006 0.38 +0.7
3,149,564 + exbB b3006 0.40 -4.3
3,149,659 + exbB b3006 0.53 -1.8
3,149,682 - exbB b3006 0.56 -3.8
3,149,682 - exbB b3006 0.56 +0.3
3,149,690 - exbB b3006 0.57 +1.3
3,149,707 + exbB b3006 0.59 -0.9
3,149,710 - exbB b3006 0.60 +0.3
3,149,718 + exbB b3006 0.61 +0.5
3,149,803 - exbB b3006 0.72 -4.2
3,149,803 - exbB b3006 0.72 -0.7
3,149,866 + exbB b3006 0.81 -3.4
3,149,956 + -3.4
3,149,956 + -2.5
3,149,956 + +1.2
3,149,983 + +2.2
3,150,016 - -0.5
3,150,257 - -0.7
3,150,288 - -3.4
3,150,292 + -5.5
3,150,300 - +2.0
3,150,300 - -2.8
3,150,337 - -4.7
3,150,378 - metC b3008 0.10 -3.8
3,150,421 + metC b3008 0.14 -3.3
3,150,421 + metC b3008 0.14 -3.6
3,150,514 - metC b3008 0.22 -2.9
3,150,575 + metC b3008 0.27 -4.3
3,150,577 - metC b3008 0.27 -5.1
3,150,583 - metC b3008 0.27 -3.4
3,150,640 - metC b3008 0.32 -4.1
3,150,654 + metC b3008 0.33 -3.7
3,150,669 + metC b3008 0.35 -4.0
3,150,706 - metC b3008 0.38 -5.5
3,150,791 - metC b3008 0.45 -4.3
3,150,820 + metC b3008 0.47 -4.5
3,150,820 + metC b3008 0.47 -4.2
3,150,912 + metC b3008 0.55 -4.5
3,150,914 + metC b3008 0.55 -2.5
3,150,915 + metC b3008 0.55 +0.1
3,150,932 - metC b3008 0.57 -2.3
3,151,015 + -0.6
3,151,015 + metC b3008 0.64 -2.9
3,151,028 + metC b3008 0.65 -5.0
3,151,038 - metC b3008 0.66 -3.2
3,151,038 - metC b3008 0.66 -4.0
3,151,074 - metC b3008 0.69 -4.7
3,151,074 - metC b3008 0.69 -4.3
3,151,120 + metC b3008 0.73 -4.9
3,151,125 + metC b3008 0.73 -1.1
3,151,138 - metC b3008 0.74 -3.7
3,151,216 + metC b3008 0.81 -3.4
3,151,216 + metC b3008 0.81 -4.2
3,151,237 + metC b3008 0.82 -3.4
3,151,274 - metC b3008 0.86 -5.1
3,151,376 + -4.2
3,151,655 - yghB b3009 0.11 -1.7
3,151,859 + yghB b3009 0.42 +0.3
3,151,859 + yghB b3009 0.42 -3.5
3,151,867 - yghB b3009 0.43 -0.5
3,151,889 + yghB b3009 0.46 -1.0
3,151,889 + yghB b3009 0.46 +1.8
3,152,046 + yghB b3009 0.70 -3.0
3,152,046 + yghB b3009 0.70 +0.4
3,152,076 + yghB b3009 0.74 -4.4
3,152,084 - yghB b3009 0.76 +0.0
3,152,101 - yghB b3009 0.78 -2.0
3,152,101 - yghB b3009 0.78 -2.9
3,152,130 + yghB b3009 0.83 -0.1
3,152,133 + yghB b3009 0.83 -0.4
3,152,152 - yghB b3009 0.86 -0.7
3,152,153 - yghB b3009 0.86 -0.0
3,152,186 + +0.1
3,152,256 + +1.4
3,152,256 + +0.5
3,152,280 + +0.6
3,152,290 + +2.5
3,152,290 + -3.3
3,152,372 + -0.4
3,152,377 + +0.3

Or see this region's nucleotide sequence