Strain Fitness in Escherichia coli BW25113 around b2987

Experiment: carbon source D-Glucose 0.25mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyghS and yghT are separated by 173 nucleotidesyghT and pitB are separated by 48 nucleotidespitB and gss are separated by 291 nucleotides b2985: yghS - predicted protein with nucleoside triphosphate hydrolase domain (NCBI), at 3,131,266 to 3,131,979 yghS b2986: yghT - predicted protein with nucleoside triphosphate hydrolase domain (NCBI), at 3,132,153 to 3,132,845 yghT b2987: pitB - phosphate transporter (NCBI), at 3,132,894 to 3,134,393 pitB b2988: gss - fused glutathionylspermidine amidase/glutathionylspermidine synthetase (NCBI), at 3,134,685 to 3,136,544 gss Position (kb) 3132 3133 3134 3135Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4 5at 3131.897 kb on + strand, within yghSat 3131.902 kb on + strand, within yghSat 3131.909 kb on + strandat 3131.909 kb on + strandat 3131.909 kb on + strandat 3131.910 kb on + strandat 3131.911 kb on + strandat 3131.911 kb on + strandat 3131.915 kb on + strandat 3131.919 kb on + strandat 3131.935 kb on + strandat 3132.208 kb on + strandat 3132.212 kb on - strandat 3132.214 kb on - strandat 3132.216 kb on + strandat 3132.216 kb on + strandat 3132.216 kb on + strandat 3132.216 kb on + strandat 3132.216 kb on - strandat 3132.216 kb on - strandat 3132.224 kb on - strand, within yghTat 3132.225 kb on - strand, within yghTat 3132.225 kb on - strand, within yghTat 3132.225 kb on - strand, within yghTat 3132.225 kb on - strand, within yghTat 3132.225 kb on - strand, within yghTat 3132.225 kb on - strand, within yghTat 3132.235 kb on - strand, within yghTat 3132.292 kb on - strand, within yghTat 3132.346 kb on - strand, within yghTat 3132.412 kb on - strand, within yghTat 3132.417 kb on - strand, within yghTat 3132.417 kb on - strand, within yghTat 3132.417 kb on - strand, within yghTat 3132.432 kb on - strand, within yghTat 3132.442 kb on - strand, within yghTat 3132.450 kb on - strand, within yghTat 3132.452 kb on - strand, within yghTat 3132.454 kb on - strand, within yghTat 3132.454 kb on - strand, within yghTat 3132.527 kb on - strand, within yghTat 3132.531 kb on + strand, within yghTat 3132.536 kb on + strand, within yghTat 3132.536 kb on + strand, within yghTat 3132.543 kb on - strand, within yghTat 3132.548 kb on + strand, within yghTat 3132.575 kb on - strand, within yghTat 3132.575 kb on - strand, within yghTat 3132.622 kb on + strand, within yghTat 3132.742 kb on + strand, within yghTat 3132.767 kb on + strand, within yghTat 3132.875 kb on - strandat 3132.875 kb on - strandat 3132.875 kb on - strandat 3132.905 kb on + strandat 3132.913 kb on + strandat 3132.913 kb on + strandat 3132.916 kb on - strandat 3132.916 kb on - strandat 3132.988 kb on + strandat 3133.073 kb on + strand, within pitBat 3133.073 kb on + strand, within pitBat 3133.136 kb on + strand, within pitBat 3133.231 kb on + strand, within pitBat 3133.251 kb on + strand, within pitBat 3133.251 kb on + strand, within pitBat 3133.287 kb on + strand, within pitBat 3133.369 kb on - strand, within pitBat 3133.383 kb on + strand, within pitBat 3133.411 kb on - strand, within pitBat 3133.411 kb on - strand, within pitBat 3133.556 kb on + strand, within pitBat 3133.568 kb on + strand, within pitBat 3133.568 kb on + strand, within pitBat 3133.579 kb on + strand, within pitBat 3133.600 kb on + strand, within pitBat 3133.600 kb on + strand, within pitBat 3133.649 kb on + strand, within pitBat 3133.690 kb on - strand, within pitBat 3133.849 kb on + strand, within pitBat 3133.852 kb on + strand, within pitBat 3133.852 kb on + strand, within pitBat 3133.893 kb on - strand, within pitBat 3133.898 kb on - strand, within pitBat 3133.911 kb on - strand, within pitBat 3133.974 kb on - strand, within pitBat 3134.028 kb on - strand, within pitBat 3134.052 kb on + strand, within pitBat 3134.065 kb on - strand, within pitBat 3134.098 kb on + strand, within pitBat 3134.098 kb on + strand, within pitBat 3134.115 kb on + strand, within pitBat 3134.126 kb on + strand, within pitBat 3134.133 kb on + strand, within pitBat 3134.133 kb on + strand, within pitBat 3134.156 kb on + strand, within pitBat 3134.156 kb on + strand, within pitBat 3134.169 kb on + strand, within pitBat 3134.169 kb on + strandat 3134.169 kb on + strand, within pitBat 3134.180 kb on + strand, within pitBat 3134.180 kb on + strand, within pitBat 3134.180 kb on + strand, within pitBat 3134.183 kb on - strand, within pitBat 3134.183 kb on - strand, within pitBat 3134.190 kb on + strand, within pitBat 3134.224 kb on + strand, within pitBat 3134.231 kb on + strand, within pitBat 3134.231 kb on + strand, within pitBat 3134.253 kb on + strandat 3134.259 kb on + strandat 3134.259 kb on + strandat 3134.259 kb on + strandat 3134.259 kb on + strandat 3134.260 kb on + strandat 3134.302 kb on + strandat 3134.311 kb on + strandat 3134.311 kb on + strandat 3134.314 kb on + strandat 3134.327 kb on + strandat 3134.330 kb on + strandat 3134.330 kb on + strandat 3134.359 kb on + strandat 3134.359 kb on + strandat 3134.359 kb on + strandat 3134.359 kb on + strandat 3134.359 kb on + strandat 3134.359 kb on + strandat 3134.361 kb on + strandat 3134.361 kb on + strandat 3134.361 kb on + strandat 3134.361 kb on + strandat 3134.361 kb on + strandat 3134.361 kb on + strandat 3134.361 kb on + strandat 3134.431 kb on + strandat 3134.431 kb on + strandat 3134.652 kb on - strandat 3134.654 kb on - strandat 3134.654 kb on - strandat 3134.670 kb on - strandat 3134.670 kb on - strandat 3134.670 kb on - strandat 3134.670 kb on - strandat 3134.670 kb on - strandat 3134.670 kb on - strandat 3134.670 kb on - strandat 3134.670 kb on - strandat 3134.670 kb on - strandat 3134.670 kb on - strandat 3134.670 kb on - strandat 3134.680 kb on - strandat 3134.680 kb on - strandat 3134.680 kb on - strandat 3134.680 kb on - strandat 3134.681 kb on - strandat 3134.681 kb on - strandat 3134.743 kb on - strandat 3134.744 kb on - strandat 3134.749 kb on - strandat 3134.749 kb on - strandat 3134.749 kb on - strandat 3134.768 kb on - strandat 3134.768 kb on - strandat 3134.770 kb on - strandat 3134.770 kb on - strandat 3134.785 kb on + strandat 3134.785 kb on - strandat 3134.785 kb on - strandat 3134.794 kb on - strandat 3134.830 kb on + strandat 3134.886 kb on - strand, within gssat 3134.925 kb on - strand, within gssat 3135.269 kb on + strand, within gssat 3135.269 kb on + strand, within gssat 3135.283 kb on - strand, within gssat 3135.304 kb on + strandat 3135.336 kb on - strand, within gssat 3135.389 kb on + strand, within gss

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.25mM
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3,131,897 + yghS b2985 0.88 +1.3
3,131,902 + yghS b2985 0.89 +0.5
3,131,909 + -2.6
3,131,909 + +0.1
3,131,909 + -3.1
3,131,910 + +2.7
3,131,911 + -1.1
3,131,911 + +2.7
3,131,915 + +1.8
3,131,919 + +0.7
3,131,935 + -2.9
3,132,208 + +2.1
3,132,212 - -0.5
3,132,214 - -0.2
3,132,216 + +2.1
3,132,216 + +1.5
3,132,216 + +3.1
3,132,216 + +2.1
3,132,216 - +1.5
3,132,216 - -0.7
3,132,224 - yghT b2986 0.10 +4.9
3,132,225 - yghT b2986 0.10 +1.1
3,132,225 - yghT b2986 0.10 +0.1
3,132,225 - yghT b2986 0.10 -2.0
3,132,225 - yghT b2986 0.10 -0.5
3,132,225 - yghT b2986 0.10 -2.0
3,132,225 - yghT b2986 0.10 +3.1
3,132,235 - yghT b2986 0.12 +4.9
3,132,292 - yghT b2986 0.20 -3.5
3,132,346 - yghT b2986 0.28 -1.0
3,132,412 - yghT b2986 0.37 -0.8
3,132,417 - yghT b2986 0.38 +0.3
3,132,417 - yghT b2986 0.38 -2.0
3,132,417 - yghT b2986 0.38 +0.7
3,132,432 - yghT b2986 0.40 +1.6
3,132,442 - yghT b2986 0.42 -3.7
3,132,450 - yghT b2986 0.43 +0.2
3,132,452 - yghT b2986 0.43 -2.9
3,132,454 - yghT b2986 0.43 -2.9
3,132,454 - yghT b2986 0.43 -2.4
3,132,527 - yghT b2986 0.54 +0.2
3,132,531 + yghT b2986 0.55 -0.3
3,132,536 + yghT b2986 0.55 -3.6
3,132,536 + yghT b2986 0.55 -1.4
3,132,543 - yghT b2986 0.56 +0.0
3,132,548 + yghT b2986 0.57 -0.6
3,132,575 - yghT b2986 0.61 -1.2
3,132,575 - yghT b2986 0.61 -2.0
3,132,622 + yghT b2986 0.68 -0.4
3,132,742 + yghT b2986 0.85 +0.3
3,132,767 + yghT b2986 0.89 +1.6
3,132,875 - -2.6
3,132,875 - -0.9
3,132,875 - -3.0
3,132,905 + +0.9
3,132,913 + -2.6
3,132,913 + -2.7
3,132,916 - +1.3
3,132,916 - +0.9
3,132,988 + -4.6
3,133,073 + pitB b2987 0.12 +0.4
3,133,073 + pitB b2987 0.12 -0.2
3,133,136 + pitB b2987 0.16 +0.4
3,133,231 + pitB b2987 0.22 +0.4
3,133,251 + pitB b2987 0.24 +0.7
3,133,251 + pitB b2987 0.24 -0.1
3,133,287 + pitB b2987 0.26 -0.1
3,133,369 - pitB b2987 0.32 +1.4
3,133,383 + pitB b2987 0.33 +1.8
3,133,411 - pitB b2987 0.34 -3.5
3,133,411 - pitB b2987 0.34 +1.3
3,133,556 + pitB b2987 0.44 -3.7
3,133,568 + pitB b2987 0.45 +1.1
3,133,568 + pitB b2987 0.45 -4.2
3,133,579 + pitB b2987 0.46 -0.6
3,133,600 + pitB b2987 0.47 -0.7
3,133,600 + pitB b2987 0.47 -1.7
3,133,649 + pitB b2987 0.50 +1.3
3,133,690 - pitB b2987 0.53 -0.1
3,133,849 + pitB b2987 0.64 -4.1
3,133,852 + pitB b2987 0.64 +3.5
3,133,852 + pitB b2987 0.64 -1.2
3,133,893 - pitB b2987 0.67 -2.8
3,133,898 - pitB b2987 0.67 -0.1
3,133,911 - pitB b2987 0.68 -2.7
3,133,974 - pitB b2987 0.72 -2.2
3,134,028 - pitB b2987 0.76 -1.9
3,134,052 + pitB b2987 0.77 +1.1
3,134,065 - pitB b2987 0.78 -1.6
3,134,098 + pitB b2987 0.80 -1.4
3,134,098 + pitB b2987 0.80 -1.5
3,134,115 + pitB b2987 0.81 -3.4
3,134,126 + pitB b2987 0.82 +1.0
3,134,133 + pitB b2987 0.83 -3.7
3,134,133 + pitB b2987 0.83 -3.5
3,134,156 + pitB b2987 0.84 +0.8
3,134,156 + pitB b2987 0.84 +0.2
3,134,169 + pitB b2987 0.85 -2.8
3,134,169 + -0.6
3,134,169 + pitB b2987 0.85 +0.8
3,134,180 + pitB b2987 0.86 -3.0
3,134,180 + pitB b2987 0.86 -0.7
3,134,180 + pitB b2987 0.86 -3.2
3,134,183 - pitB b2987 0.86 +1.5
3,134,183 - pitB b2987 0.86 -0.5
3,134,190 + pitB b2987 0.86 +0.6
3,134,224 + pitB b2987 0.89 -4.0
3,134,231 + pitB b2987 0.89 +0.5
3,134,231 + pitB b2987 0.89 -4.4
3,134,253 + -4.3
3,134,259 + +1.2
3,134,259 + -1.4
3,134,259 + -3.0
3,134,259 + +2.0
3,134,260 + +0.3
3,134,302 + -1.7
3,134,311 + -2.1
3,134,311 + -0.9
3,134,314 + +1.8
3,134,327 + +2.6
3,134,330 + +0.9
3,134,330 + -3.8
3,134,359 + -1.2
3,134,359 + -3.2
3,134,359 + -1.4
3,134,359 + -0.5
3,134,359 + -3.6
3,134,359 + +2.4
3,134,361 + +0.1
3,134,361 + -1.7
3,134,361 + +1.3
3,134,361 + +2.6
3,134,361 + -2.7
3,134,361 + -1.9
3,134,361 + -4.3
3,134,431 + +3.0
3,134,431 + -1.3
3,134,652 - -2.5
3,134,654 - +0.3
3,134,654 - -1.6
3,134,670 - +0.1
3,134,670 - -0.9
3,134,670 - -4.1
3,134,670 - +2.8
3,134,670 - -0.6
3,134,670 - +0.2
3,134,670 - +1.2
3,134,670 - +0.8
3,134,670 - -0.1
3,134,670 - +1.0
3,134,670 - +0.7
3,134,680 - +2.3
3,134,680 - -3.5
3,134,680 - -0.6
3,134,680 - -1.2
3,134,681 - +0.4
3,134,681 - -0.8
3,134,743 - +1.5
3,134,744 - +2.2
3,134,749 - +0.9
3,134,749 - +1.2
3,134,749 - +1.7
3,134,768 - +0.7
3,134,768 - -1.4
3,134,770 - -1.9
3,134,770 - -3.0
3,134,785 + +0.5
3,134,785 - +0.3
3,134,785 - +1.8
3,134,794 - +1.9
3,134,830 + +1.1
3,134,886 - gss b2988 0.11 +0.2
3,134,925 - gss b2988 0.13 -4.9
3,135,269 + gss b2988 0.31 +0.4
3,135,269 + gss b2988 0.31 -3.6
3,135,283 - gss b2988 0.32 -1.6
3,135,304 + -0.7
3,135,336 - gss b2988 0.35 -5.1
3,135,389 + gss b2988 0.38 +0.3

Or see this region's nucleotide sequence