Experiment: carbon source D-Glucose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lsrB and lsrF are separated by 26 nucleotides lsrF and lsrG are separated by 23 nucleotides lsrG and tam are separated by 56 nucleotides
b1516: lsrB - AI2 transporter (NCBI), at 1,603,075 to 1,604,097
lsrB
b1517: lsrF - hypothetical protein (NCBI), at 1,604,124 to 1,604,999
lsrF
b1518: lsrG - autoinducer-2 (AI-2) modifying protein LsrG (NCBI), at 1,605,023 to 1,605,313
lsrG
b1519: tam - trans-aconitate 2-methyltransferase (NCBI), at 1,605,370 to 1,606,128
tam
Position (kb)
1604
1605 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5
6 at 1603.134 kb on + strand at 1603.408 kb on - strand, within lsrB at 1603.487 kb on - strand, within lsrB at 1603.659 kb on - strand, within lsrB at 1603.832 kb on + strand, within lsrB at 1603.832 kb on + strand, within lsrB at 1603.997 kb on + strand at 1603.999 kb on + strand at 1603.999 kb on + strand at 1603.999 kb on + strand at 1604.043 kb on + strand at 1604.043 kb on + strand at 1604.044 kb on + strand at 1604.191 kb on + strand at 1604.214 kb on + strand, within lsrF at 1604.214 kb on + strand, within lsrF at 1604.232 kb on + strand, within lsrF at 1604.238 kb on + strand, within lsrF at 1604.238 kb on + strand, within lsrF at 1604.246 kb on + strand, within lsrF at 1604.325 kb on - strand, within lsrF at 1604.325 kb on - strand, within lsrF at 1604.331 kb on - strand, within lsrF at 1604.404 kb on + strand, within lsrF at 1604.423 kb on + strand, within lsrF at 1604.433 kb on + strand, within lsrF at 1604.441 kb on - strand, within lsrF at 1604.441 kb on - strand, within lsrF at 1604.501 kb on - strand, within lsrF at 1604.527 kb on + strand, within lsrF at 1604.623 kb on - strand, within lsrF at 1604.636 kb on + strand, within lsrF at 1604.637 kb on + strand, within lsrF at 1604.637 kb on + strand, within lsrF at 1604.637 kb on + strand, within lsrF at 1604.712 kb on - strand, within lsrF at 1604.785 kb on + strand, within lsrF at 1604.866 kb on - strand, within lsrF at 1604.866 kb on - strand, within lsrF at 1604.871 kb on - strand, within lsrF at 1605.110 kb on + strand, within lsrG at 1605.118 kb on - strand, within lsrG at 1605.548 kb on - strand, within tam at 1605.778 kb on - strand, within tam at 1605.917 kb on + strand, within tam
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.25mM remove 1,603,134 + +1.0 1,603,408 - lsrB b1516 0.33 +0.6 1,603,487 - lsrB b1516 0.40 +2.1 1,603,659 - lsrB b1516 0.57 +0.7 1,603,832 + lsrB b1516 0.74 +0.9 1,603,832 + lsrB b1516 0.74 +0.4 1,603,997 + +1.0 1,603,999 + -0.1 1,603,999 + -0.3 1,603,999 + +1.0 1,604,043 + +1.0 1,604,043 + -4.3 1,604,044 + +0.6 1,604,191 + -4.0 1,604,214 + lsrF b1517 0.10 -3.9 1,604,214 + lsrF b1517 0.10 +1.3 1,604,232 + lsrF b1517 0.12 -4.0 1,604,238 + lsrF b1517 0.13 -4.0 1,604,238 + lsrF b1517 0.13 +1.6 1,604,246 + lsrF b1517 0.14 +0.1 1,604,325 - lsrF b1517 0.23 +0.6 1,604,325 - lsrF b1517 0.23 +0.1 1,604,331 - lsrF b1517 0.24 +0.1 1,604,404 + lsrF b1517 0.32 +1.7 1,604,423 + lsrF b1517 0.34 -0.9 1,604,433 + lsrF b1517 0.35 -3.3 1,604,441 - lsrF b1517 0.36 -0.2 1,604,441 - lsrF b1517 0.36 -0.4 1,604,501 - lsrF b1517 0.43 -2.9 1,604,527 + lsrF b1517 0.46 +1.0 1,604,623 - lsrF b1517 0.57 -2.1 1,604,636 + lsrF b1517 0.58 +0.6 1,604,637 + lsrF b1517 0.59 +1.6 1,604,637 + lsrF b1517 0.59 -4.0 1,604,637 + lsrF b1517 0.59 +1.3 1,604,712 - lsrF b1517 0.67 -3.0 1,604,785 + lsrF b1517 0.75 +2.2 1,604,866 - lsrF b1517 0.85 -1.8 1,604,866 - lsrF b1517 0.85 +6.5 1,604,871 - lsrF b1517 0.85 +1.6 1,605,110 + lsrG b1518 0.30 +0.0 1,605,118 - lsrG b1518 0.33 +1.1 1,605,548 - tam b1519 0.23 -2.3 1,605,778 - tam b1519 0.54 -5.1 1,605,917 + tam b1519 0.72 -3.5
Or see this region's nucleotide sequence