Experiment: carbon source D-Glucose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nohB and ylcF overlap by 26 nucleotides ylcF and tfaD overlap by 3 nucleotides tfaD and ybcY are separated by 54 nucleotides ybcY and tfaX are separated by 146 nucleotides tfaX and appY are separated by 545 nucleotides
b0560: nohB - DLP12 prophage; DNA packaging protein (NCBI), at 580,057 to 580,602
nohB
b4634: ylcF - no description, at 580,577 to 580,885
ylcF
b0561: tfaD - orf, hypothetical protein (VIMSS), at 580,883 to 581,320
tfaD
b0562: ybcY - orf, hypothetical protein (VIMSS), at 581,375 to 582,029
ybcY
b0563: tfaX - DLP12 prophage; predicted protein (RefSeq), at 582,176 to 582,358
tfaX
b0564: appY - DLP12 prophage; DNA-binding transcriptional activator (NCBI), at 582,904 to 583,653
appY
Position (kb) 581
582
583 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 580.605 kb on - strand at 580.642 kb on + strand, within ylcF at 580.668 kb on - strand, within ylcF at 580.668 kb on - strand, within ylcF at 580.733 kb on + strand, within ylcF at 580.733 kb on + strand, within ylcF at 580.733 kb on + strand, within ylcF at 580.944 kb on - strand, within tfaD at 580.973 kb on + strand, within tfaD at 580.981 kb on - strand, within tfaD at 580.981 kb on - strand, within tfaD at 581.152 kb on + strand, within tfaD at 581.171 kb on + strand, within tfaD at 581.192 kb on - strand, within tfaD at 581.192 kb on - strand, within tfaD at 581.252 kb on + strand, within tfaD at 581.260 kb on - strand, within tfaD at 581.260 kb on - strand, within tfaD at 581.262 kb on + strand, within tfaD at 581.269 kb on - strand, within tfaD at 581.285 kb on + strand at 581.307 kb on + strand at 581.307 kb on + strand at 581.343 kb on + strand at 581.344 kb on + strand at 581.344 kb on + strand at 581.352 kb on - strand at 581.404 kb on + strand at 581.411 kb on + strand at 581.443 kb on + strand, within ybcY at 581.450 kb on + strand, within ybcY at 581.453 kb on - strand, within ybcY at 581.460 kb on + strand, within ybcY at 581.524 kb on + strand, within ybcY at 581.524 kb on + strand, within ybcY at 581.572 kb on + strand, within ybcY at 581.574 kb on + strand, within ybcY at 581.574 kb on + strand, within ybcY at 581.576 kb on + strand, within ybcY at 581.576 kb on + strand, within ybcY at 581.584 kb on - strand, within ybcY at 581.584 kb on - strand, within ybcY at 581.595 kb on + strand, within ybcY at 581.603 kb on - strand, within ybcY at 581.654 kb on + strand, within ybcY at 581.654 kb on + strand, within ybcY at 581.665 kb on - strand, within ybcY at 581.672 kb on - strand, within ybcY at 581.701 kb on - strand, within ybcY at 581.704 kb on - strand, within ybcY at 581.704 kb on - strand, within ybcY at 581.704 kb on - strand, within ybcY at 581.717 kb on - strand, within ybcY at 581.766 kb on + strand, within ybcY at 581.772 kb on + strand, within ybcY at 581.772 kb on - strand, within ybcY at 581.772 kb on - strand, within ybcY at 581.774 kb on - strand, within ybcY at 581.785 kb on + strand, within ybcY at 581.816 kb on - strand, within ybcY at 581.822 kb on + strand, within ybcY at 581.830 kb on - strand, within ybcY at 581.831 kb on + strand, within ybcY at 581.836 kb on + strand, within ybcY at 581.836 kb on + strand, within ybcY at 581.841 kb on + strand, within ybcY at 581.841 kb on + strand, within ybcY at 581.841 kb on - strand, within ybcY at 581.862 kb on - strand, within ybcY at 581.862 kb on - strand, within ybcY at 581.876 kb on + strand, within ybcY at 581.876 kb on + strand, within ybcY at 581.877 kb on - strand, within ybcY at 581.877 kb on - strand, within ybcY at 581.884 kb on - strand, within ybcY at 581.890 kb on - strand, within ybcY at 581.900 kb on + strand, within ybcY at 581.900 kb on + strand, within ybcY at 581.920 kb on + strand, within ybcY at 581.920 kb on + strand, within ybcY at 581.920 kb on - strand, within ybcY at 581.925 kb on - strand, within ybcY at 581.925 kb on - strand, within ybcY at 581.928 kb on - strand, within ybcY at 581.928 kb on - strand, within ybcY at 581.930 kb on + strand, within ybcY at 581.930 kb on + strand, within ybcY at 581.930 kb on + strand, within ybcY at 581.930 kb on + strand, within ybcY at 581.930 kb on + strand, within ybcY at 581.932 kb on + strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.938 kb on - strand, within ybcY at 581.940 kb on + strand, within ybcY at 581.969 kb on + strand at 581.970 kb on + strand at 581.970 kb on + strand at 581.970 kb on + strand at 581.977 kb on + strand at 581.977 kb on + strand at 581.992 kb on - strand at 582.013 kb on + strand at 582.104 kb on + strand at 582.104 kb on + strand at 582.138 kb on + strand at 582.140 kb on - strand at 582.147 kb on + strand at 582.147 kb on + strand at 582.151 kb on - strand at 582.155 kb on - strand at 582.163 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.174 kb on + strand at 582.176 kb on + strand at 582.177 kb on + strand at 582.182 kb on - strand at 582.182 kb on - strand at 582.182 kb on - strand at 582.182 kb on - strand at 582.182 kb on - strand at 582.182 kb on - strand at 582.184 kb on + strand at 582.184 kb on + strand at 582.185 kb on - strand at 582.187 kb on - strand at 582.190 kb on - strand at 582.190 kb on - strand at 582.208 kb on + strand, within tfaX at 582.208 kb on + strand, within tfaX at 582.210 kb on + strand, within tfaX at 582.210 kb on + strand, within tfaX at 582.220 kb on + strand, within tfaX at 582.220 kb on + strand, within tfaX at 582.221 kb on + strand, within tfaX at 582.221 kb on + strand, within tfaX at 582.226 kb on + strand, within tfaX at 582.236 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.299 kb on + strand, within tfaX at 582.301 kb on + strand, within tfaX at 582.306 kb on + strand, within tfaX at 582.306 kb on + strand, within tfaX at 582.307 kb on + strand, within tfaX at 582.318 kb on + strand, within tfaX at 582.318 kb on + strand, within tfaX at 582.326 kb on + strand, within tfaX at 583.029 kb on + strand, within appY
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.25mM remove 580,605 - +0.7 580,642 + ylcF b4634 0.21 -0.6 580,668 - ylcF b4634 0.29 +0.0 580,668 - ylcF b4634 0.29 +0.8 580,733 + ylcF b4634 0.50 -1.0 580,733 + ylcF b4634 0.50 -0.2 580,733 + ylcF b4634 0.50 +1.2 580,944 - tfaD b0561 0.14 -0.9 580,973 + tfaD b0561 0.21 -4.0 580,981 - tfaD b0561 0.22 +1.5 580,981 - tfaD b0561 0.22 +1.7 581,152 + tfaD b0561 0.61 +0.8 581,171 + tfaD b0561 0.66 +1.0 581,192 - tfaD b0561 0.71 -2.5 581,192 - tfaD b0561 0.71 +0.5 581,252 + tfaD b0561 0.84 +1.0 581,260 - tfaD b0561 0.86 -1.2 581,260 - tfaD b0561 0.86 +1.8 581,262 + tfaD b0561 0.87 -5.0 581,269 - tfaD b0561 0.88 +1.3 581,285 + +0.4 581,307 + +1.2 581,307 + +0.9 581,343 + +1.1 581,344 + +1.4 581,344 + -3.1 581,352 - +1.3 581,404 + -2.6 581,411 + +0.8 581,443 + ybcY b0562 0.10 -3.9 581,450 + ybcY b0562 0.11 -2.2 581,453 - ybcY b0562 0.12 +0.4 581,460 + ybcY b0562 0.13 -2.7 581,524 + ybcY b0562 0.23 -1.8 581,524 + ybcY b0562 0.23 -1.2 581,572 + ybcY b0562 0.30 -1.3 581,574 + ybcY b0562 0.30 -0.7 581,574 + ybcY b0562 0.30 -3.0 581,576 + ybcY b0562 0.31 -2.4 581,576 + ybcY b0562 0.31 -2.2 581,584 - ybcY b0562 0.32 -2.2 581,584 - ybcY b0562 0.32 -2.7 581,595 + ybcY b0562 0.34 -0.7 581,603 - ybcY b0562 0.35 +1.5 581,654 + ybcY b0562 0.43 -1.9 581,654 + ybcY b0562 0.43 +0.0 581,665 - ybcY b0562 0.44 -3.1 581,672 - ybcY b0562 0.45 +1.1 581,701 - ybcY b0562 0.50 +2.4 581,704 - ybcY b0562 0.50 -3.9 581,704 - ybcY b0562 0.50 +0.4 581,704 - ybcY b0562 0.50 -4.7 581,717 - ybcY b0562 0.52 +1.4 581,766 + ybcY b0562 0.60 +2.8 581,772 + ybcY b0562 0.61 -1.7 581,772 - ybcY b0562 0.61 +1.1 581,772 - ybcY b0562 0.61 +2.0 581,774 - ybcY b0562 0.61 +2.0 581,785 + ybcY b0562 0.63 -0.2 581,816 - ybcY b0562 0.67 -1.9 581,822 + ybcY b0562 0.68 -2.3 581,830 - ybcY b0562 0.69 -1.9 581,831 + ybcY b0562 0.70 +0.0 581,836 + ybcY b0562 0.70 -1.9 581,836 + ybcY b0562 0.70 -2.7 581,841 + ybcY b0562 0.71 -1.2 581,841 + ybcY b0562 0.71 +0.5 581,841 - ybcY b0562 0.71 +1.5 581,862 - ybcY b0562 0.74 -1.7 581,862 - ybcY b0562 0.74 +1.4 581,876 + ybcY b0562 0.76 -1.4 581,876 + ybcY b0562 0.76 +2.3 581,877 - ybcY b0562 0.77 -2.2 581,877 - ybcY b0562 0.77 -2.8 581,884 - ybcY b0562 0.78 -3.2 581,890 - ybcY b0562 0.79 -2.7 581,900 + ybcY b0562 0.80 -1.7 581,900 + ybcY b0562 0.80 -1.3 581,920 + ybcY b0562 0.83 -2.3 581,920 + ybcY b0562 0.83 +1.8 581,920 - ybcY b0562 0.83 -1.2 581,925 - ybcY b0562 0.84 +2.5 581,925 - ybcY b0562 0.84 -1.9 581,928 - ybcY b0562 0.84 -0.9 581,928 - ybcY b0562 0.84 +1.7 581,930 + ybcY b0562 0.85 -2.6 581,930 + ybcY b0562 0.85 +0.9 581,930 + ybcY b0562 0.85 -2.2 581,930 + ybcY b0562 0.85 -1.9 581,930 + ybcY b0562 0.85 -0.8 581,932 + ybcY b0562 0.85 +0.4 581,938 - ybcY b0562 0.86 -2.4 581,938 - ybcY b0562 0.86 -1.1 581,938 - ybcY b0562 0.86 +1.7 581,938 - ybcY b0562 0.86 -1.8 581,938 - ybcY b0562 0.86 +3.2 581,938 - ybcY b0562 0.86 +2.8 581,938 - ybcY b0562 0.86 +1.1 581,940 + ybcY b0562 0.86 +0.7 581,969 + +1.9 581,970 + +1.2 581,970 + +0.9 581,970 + -2.6 581,977 + -2.3 581,977 + -2.3 581,992 - +1.3 582,013 + -0.3 582,104 + -2.2 582,104 + -3.0 582,138 + -4.3 582,140 - +2.8 582,147 + -1.2 582,147 + -2.0 582,151 - -1.5 582,155 - -1.2 582,163 + +1.7 582,174 + -1.3 582,174 + +2.0 582,174 + -0.2 582,174 + -3.5 582,174 + +1.0 582,174 + +1.2 582,174 + +0.7 582,176 + -0.1 582,177 + +0.6 582,182 - -0.2 582,182 - +1.3 582,182 - +2.8 582,182 - +2.8 582,182 - -2.0 582,182 - +0.0 582,184 + -2.8 582,184 + +0.1 582,185 - +2.8 582,187 - +1.8 582,190 - +2.8 582,190 - +2.8 582,208 + tfaX b0563 0.17 +1.1 582,208 + tfaX b0563 0.17 -2.0 582,210 + tfaX b0563 0.19 -2.1 582,210 + tfaX b0563 0.19 +1.6 582,220 + tfaX b0563 0.24 -2.9 582,220 + tfaX b0563 0.24 -0.1 582,221 + tfaX b0563 0.25 +0.9 582,221 + tfaX b0563 0.25 +2.0 582,226 + tfaX b0563 0.27 -2.8 582,236 + tfaX b0563 0.33 -0.4 582,299 + tfaX b0563 0.67 -1.1 582,299 + tfaX b0563 0.67 -2.2 582,299 + tfaX b0563 0.67 -2.5 582,299 + tfaX b0563 0.67 -1.7 582,299 + tfaX b0563 0.67 -1.2 582,299 + tfaX b0563 0.67 +0.9 582,299 + tfaX b0563 0.67 -3.2 582,299 + tfaX b0563 0.67 +2.0 582,299 + tfaX b0563 0.67 +1.7 582,301 + tfaX b0563 0.68 -1.9 582,306 + tfaX b0563 0.71 -2.2 582,306 + tfaX b0563 0.71 +3.3 582,307 + tfaX b0563 0.72 -2.1 582,318 + tfaX b0563 0.78 +1.7 582,318 + tfaX b0563 0.78 -1.1 582,326 + tfaX b0563 0.82 -2.1 583,029 + appY b0564 0.17 +2.8
Or see this region's nucleotide sequence