Experiment: carbon source D-Glucose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yahF and yahG overlap by 1 nucleotides yahG and yahI are separated by 421 nucleotides
b0320: yahF - predicted acyl-CoA synthetase with NAD(P)-binding domain and succinyl-CoA synthetase domain (NCBI), at 336,002 to 337,549
yahF
b0321: yahG - hypothetical protein (NCBI), at 337,549 to 338,967
yahG
b0323: yahI - predicted carbamate kinase-like protein (NCBI), at 339,389 to 340,339
yahI
Position (kb) 337
338
339 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 336.554 kb on - strand, within yahF at 337.024 kb on - strand, within yahF at 337.163 kb on - strand, within yahF at 337.169 kb on + strand, within yahF at 337.169 kb on + strand, within yahF at 337.236 kb on - strand, within yahF at 337.603 kb on + strand at 337.603 kb on + strand at 337.763 kb on + strand, within yahG at 337.763 kb on + strand, within yahG at 337.821 kb on + strand, within yahG at 337.829 kb on - strand, within yahG at 337.974 kb on - strand, within yahG at 337.974 kb on - strand, within yahG at 338.157 kb on + strand, within yahG at 338.157 kb on + strand, within yahG at 338.178 kb on + strand, within yahG at 338.183 kb on + strand, within yahG at 338.183 kb on + strand, within yahG at 338.188 kb on + strand, within yahG at 338.196 kb on - strand, within yahG at 338.196 kb on - strand, within yahG at 338.231 kb on + strand, within yahG at 338.237 kb on - strand, within yahG at 338.266 kb on + strand, within yahG at 338.365 kb on + strand, within yahG at 338.365 kb on + strand, within yahG at 338.424 kb on - strand, within yahG at 338.429 kb on - strand, within yahG at 338.551 kb on - strand, within yahG at 338.656 kb on - strand, within yahG at 338.902 kb on + strand at 338.902 kb on + strand at 339.006 kb on - strand at 339.184 kb on - strand at 339.195 kb on + strand at 339.195 kb on + strand at 339.203 kb on - strand at 339.203 kb on - strand at 339.378 kb on - strand at 339.390 kb on - strand at 339.390 kb on - strand at 339.411 kb on - strand at 339.431 kb on + strand at 339.444 kb on - strand at 339.565 kb on + strand, within yahI at 339.565 kb on + strand, within yahI at 339.630 kb on + strand, within yahI at 339.630 kb on + strand, within yahI at 339.741 kb on - strand, within yahI at 339.798 kb on + strand, within yahI at 339.806 kb on - strand, within yahI
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.25mM remove 336,554 - yahF b0320 0.36 -2.6 337,024 - yahF b0320 0.66 -3.3 337,163 - yahF b0320 0.75 -4.1 337,169 + yahF b0320 0.75 -0.1 337,169 + yahF b0320 0.75 +0.5 337,236 - yahF b0320 0.80 +1.1 337,603 + +1.6 337,603 + -0.3 337,763 + yahG b0321 0.15 +0.8 337,763 + yahG b0321 0.15 -3.4 337,821 + yahG b0321 0.19 +1.7 337,829 - yahG b0321 0.20 -1.1 337,974 - yahG b0321 0.30 +1.3 337,974 - yahG b0321 0.30 -2.3 338,157 + yahG b0321 0.43 -3.9 338,157 + yahG b0321 0.43 -3.6 338,178 + yahG b0321 0.44 +0.2 338,183 + yahG b0321 0.45 -3.0 338,183 + yahG b0321 0.45 -3.3 338,188 + yahG b0321 0.45 +0.0 338,196 - yahG b0321 0.46 -0.3 338,196 - yahG b0321 0.46 -0.8 338,231 + yahG b0321 0.48 -0.1 338,237 - yahG b0321 0.48 -3.8 338,266 + yahG b0321 0.51 +0.0 338,365 + yahG b0321 0.58 +1.4 338,365 + yahG b0321 0.58 +1.2 338,424 - yahG b0321 0.62 -0.1 338,429 - yahG b0321 0.62 -0.0 338,551 - yahG b0321 0.71 -4.3 338,656 - yahG b0321 0.78 +0.9 338,902 + -2.0 338,902 + -1.2 339,006 - -0.0 339,184 - +0.5 339,195 + -4.3 339,195 + +1.8 339,203 - -3.5 339,203 - +1.3 339,378 - +0.4 339,390 - +0.6 339,390 - -2.4 339,411 - -0.2 339,431 + +0.2 339,444 - -4.0 339,565 + yahI b0323 0.19 +1.5 339,565 + yahI b0323 0.19 -2.1 339,630 + yahI b0323 0.25 +0.6 339,630 + yahI b0323 0.25 +1.4 339,741 - yahI b0323 0.37 +0.9 339,798 + yahI b0323 0.43 +0.6 339,806 - yahI b0323 0.44 -4.6
Or see this region's nucleotide sequence