Experiment: carbon source D-Glucose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pcnB and yadB are separated by 59 nucleotides yadB and dksA are separated by 36 nucleotides dksA and sfsA are separated by 177 nucleotides
b0143: pcnB - poly(A) polymerase I (VIMSS), at 157,729 to 159,126
pcnB
b0144: yadB - glutamyl-Q tRNA(Asp) synthetase (RefSeq), at 159,186 to 160,112
yadB
b0145: dksA - DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI), at 160,149 to 160,604
dksA
b0146: sfsA - sugar fermentation stimulation protein A (NCBI), at 160,782 to 161,486
sfsA
Position (kb) 159
160
161 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 158.227 kb on - strand, within pcnB at 158.249 kb on - strand, within pcnB at 158.249 kb on - strand, within pcnB at 158.312 kb on - strand, within pcnB at 158.342 kb on - strand, within pcnB at 158.378 kb on - strand, within pcnB at 158.400 kb on + strand, within pcnB at 158.408 kb on - strand, within pcnB at 158.408 kb on - strand, within pcnB at 158.426 kb on - strand, within pcnB at 158.442 kb on - strand, within pcnB at 158.442 kb on - strand, within pcnB at 158.447 kb on - strand, within pcnB at 158.516 kb on + strand, within pcnB at 158.522 kb on - strand, within pcnB at 158.759 kb on - strand, within pcnB at 158.759 kb on - strand, within pcnB at 158.759 kb on - strand, within pcnB at 158.864 kb on - strand, within pcnB at 159.038 kb on - strand at 159.093 kb on - strand at 159.093 kb on - strand at 159.133 kb on + strand at 159.347 kb on - strand, within yadB at 159.347 kb on - strand, within yadB at 159.352 kb on - strand, within yadB at 159.352 kb on - strand, within yadB at 159.581 kb on - strand, within yadB at 159.655 kb on + strand, within yadB at 159.699 kb on + strand, within yadB at 159.722 kb on - strand, within yadB at 159.722 kb on - strand, within yadB at 159.743 kb on - strand, within yadB at 159.754 kb on - strand, within yadB at 159.754 kb on - strand, within yadB at 159.770 kb on + strand, within yadB at 159.770 kb on + strand, within yadB at 159.803 kb on + strand, within yadB at 159.808 kb on + strand, within yadB at 159.905 kb on - strand, within yadB at 159.947 kb on + strand, within yadB at 160.046 kb on + strand at 160.068 kb on + strand at 160.068 kb on - strand at 160.068 kb on - strand at 160.070 kb on - strand at 160.078 kb on + strand at 160.078 kb on + strand at 160.194 kb on + strand at 160.198 kb on + strand, within dksA at 160.198 kb on + strand, within dksA at 160.198 kb on + strand, within dksA at 160.347 kb on - strand, within dksA at 160.391 kb on - strand, within dksA at 160.424 kb on + strand, within dksA at 160.424 kb on + strand, within dksA at 160.426 kb on - strand, within dksA at 160.465 kb on - strand, within dksA at 160.499 kb on + strand, within dksA at 160.507 kb on + strand, within dksA at 160.605 kb on - strand at 160.605 kb on - strand at 160.648 kb on - strand at 160.664 kb on + strand at 160.708 kb on - strand at 160.710 kb on + strand at 160.710 kb on + strand at 160.806 kb on + strand at 160.918 kb on + strand, within sfsA at 160.941 kb on + strand, within sfsA at 161.036 kb on + strand, within sfsA at 161.068 kb on - strand, within sfsA at 161.094 kb on + strand, within sfsA at 161.102 kb on - strand, within sfsA
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.25mM remove 158,227 - pcnB b0143 0.36 -3.1 158,249 - pcnB b0143 0.37 +1.6 158,249 - pcnB b0143 0.37 +1.2 158,312 - pcnB b0143 0.42 +0.7 158,342 - pcnB b0143 0.44 -0.7 158,378 - pcnB b0143 0.46 +1.5 158,400 + pcnB b0143 0.48 -3.0 158,408 - pcnB b0143 0.49 -3.1 158,408 - pcnB b0143 0.49 +4.1 158,426 - pcnB b0143 0.50 +1.5 158,442 - pcnB b0143 0.51 -2.0 158,442 - pcnB b0143 0.51 -2.2 158,447 - pcnB b0143 0.51 -3.2 158,516 + pcnB b0143 0.56 -2.9 158,522 - pcnB b0143 0.57 -2.0 158,759 - pcnB b0143 0.74 -2.5 158,759 - pcnB b0143 0.74 -2.5 158,759 - pcnB b0143 0.74 +0.9 158,864 - pcnB b0143 0.81 +1.9 159,038 - +1.2 159,093 - +1.0 159,093 - +0.3 159,133 + -3.8 159,347 - yadB b0144 0.17 +0.1 159,347 - yadB b0144 0.17 -1.2 159,352 - yadB b0144 0.18 -4.3 159,352 - yadB b0144 0.18 +1.5 159,581 - yadB b0144 0.43 +0.4 159,655 + yadB b0144 0.51 -3.9 159,699 + yadB b0144 0.55 -3.9 159,722 - yadB b0144 0.58 -3.3 159,722 - yadB b0144 0.58 -3.6 159,743 - yadB b0144 0.60 -1.2 159,754 - yadB b0144 0.61 -2.6 159,754 - yadB b0144 0.61 -0.2 159,770 + yadB b0144 0.63 -0.7 159,770 + yadB b0144 0.63 +0.8 159,803 + yadB b0144 0.67 +0.7 159,808 + yadB b0144 0.67 -2.1 159,905 - yadB b0144 0.78 +2.1 159,947 + yadB b0144 0.82 -5.6 160,046 + -0.6 160,068 + +0.4 160,068 - -3.0 160,068 - +1.1 160,070 - +0.4 160,078 + -3.1 160,078 + -3.9 160,194 + -3.2 160,198 + dksA b0145 0.11 -1.2 160,198 + dksA b0145 0.11 -1.4 160,198 + dksA b0145 0.11 -2.1 160,347 - dksA b0145 0.43 -2.6 160,391 - dksA b0145 0.53 -1.5 160,424 + dksA b0145 0.60 +0.4 160,424 + dksA b0145 0.60 -1.4 160,426 - dksA b0145 0.61 -0.7 160,465 - dksA b0145 0.69 +3.0 160,499 + dksA b0145 0.77 -1.6 160,507 + dksA b0145 0.79 -4.9 160,605 - -3.2 160,605 - -2.4 160,648 - -3.7 160,664 + -2.2 160,708 - -0.1 160,710 + -3.5 160,710 + -4.8 160,806 + -0.9 160,918 + sfsA b0146 0.19 +2.1 160,941 + sfsA b0146 0.23 +0.7 161,036 + sfsA b0146 0.36 -0.4 161,068 - sfsA b0146 0.41 +0.7 161,094 + sfsA b0146 0.44 -2.8 161,102 - sfsA b0146 0.45 -0.9
Or see this region's nucleotide sequence