Experiment: carbon source D-Glucose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrL and thrA are separated by 81 nucleotides thrA and thrB are separated by 1 nucleotides thrB and thrC are separated by 0 nucleotides
b0001: thrL - thr operon leader peptide (NCBI), at 190 to 255
thrL
b0002: thrA - bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI), at 337 to 2,799
thrA
b0003: thrB - homoserine kinase (NCBI), at 2,801 to 3,733
thrB
b0004: thrC - threonine synthase (NCBI), at 3,734 to 5,020
thrC
Position (kb)
0
1
2
3 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 0.070 kb on + strand at 0.070 kb on + strand at 0.078 kb on - strand at 0.078 kb on - strand at 0.161 kb on - strand at 0.185 kb on - strand at 0.185 kb on - strand at 0.208 kb on + strand, within thrL at 0.217 kb on - strand, within thrL at 0.257 kb on - strand at 0.257 kb on - strand at 0.440 kb on - strand at 0.440 kb on - strand at 0.454 kb on - strand at 0.518 kb on - strand at 0.518 kb on - strand at 0.528 kb on + strand at 0.592 kb on + strand, within thrA at 0.592 kb on + strand, within thrA at 0.666 kb on + strand, within thrA at 0.666 kb on + strand, within thrA at 0.675 kb on - strand, within thrA at 0.754 kb on - strand, within thrA at 0.754 kb on - strand, within thrA at 0.767 kb on + strand, within thrA at 0.775 kb on - strand, within thrA at 0.957 kb on + strand, within thrA at 0.957 kb on + strand, within thrA at 1.046 kb on + strand, within thrA at 1.136 kb on - strand, within thrA at 1.142 kb on - strand, within thrA at 1.161 kb on + strand, within thrA at 1.161 kb on + strand, within thrA at 1.161 kb on + strand, within thrA at 1.259 kb on - strand, within thrA at 1.259 kb on - strand, within thrA at 1.262 kb on + strand, within thrA at 1.270 kb on - strand, within thrA at 1.270 kb on - strand, within thrA at 1.280 kb on - strand, within thrA at 1.489 kb on + strand, within thrA at 1.599 kb on - strand, within thrA at 1.606 kb on + strand, within thrA at 1.627 kb on + strand, within thrA at 1.633 kb on - strand, within thrA at 1.633 kb on - strand, within thrA at 1.636 kb on + strand, within thrA at 1.636 kb on + strand, within thrA at 1.637 kb on + strand, within thrA at 1.711 kb on - strand, within thrA at 1.721 kb on - strand, within thrA at 1.767 kb on - strand, within thrA at 1.793 kb on - strand, within thrA at 1.868 kb on + strand, within thrA at 1.868 kb on + strand, within thrA at 1.871 kb on + strand, within thrA at 1.876 kb on - strand, within thrA at 1.881 kb on - strand, within thrA at 1.881 kb on - strand, within thrA at 1.929 kb on + strand, within thrA at 1.929 kb on + strand, within thrA at 1.937 kb on - strand, within thrA at 1.937 kb on - strand, within thrA at 2.076 kb on + strand, within thrA at 2.104 kb on + strand, within thrA at 2.104 kb on + strand, within thrA at 2.266 kb on - strand, within thrA at 2.266 kb on - strand, within thrA at 2.292 kb on - strand, within thrA at 2.294 kb on + strand, within thrA at 2.370 kb on - strand, within thrA at 2.370 kb on - strand, within thrA at 2.449 kb on - strand, within thrA at 2.590 kb on - strand at 2.675 kb on + strand at 2.682 kb on + strand at 2.682 kb on + strand at 2.684 kb on + strand at 2.717 kb on + strand at 2.744 kb on - strand at 2.787 kb on - strand at 2.787 kb on - strand at 2.829 kb on + strand at 2.847 kb on + strand at 2.847 kb on + strand at 2.906 kb on - strand, within thrB at 2.934 kb on + strand, within thrB at 2.963 kb on - strand, within thrB at 3.020 kb on - strand, within thrB at 3.023 kb on + strand, within thrB at 3.131 kb on + strand, within thrB at 3.144 kb on - strand, within thrB at 3.186 kb on + strand, within thrB at 3.246 kb on + strand, within thrB at 3.248 kb on + strand, within thrB at 3.256 kb on + strand, within thrB at 3.293 kb on + strand, within thrB at 3.301 kb on - strand, within thrB at 3.318 kb on - strand, within thrB at 3.411 kb on + strand, within thrB at 3.444 kb on - strand, within thrB at 3.444 kb on - strand, within thrB at 3.504 kb on - strand, within thrB at 3.504 kb on - strand, within thrB at 3.509 kb on - strand, within thrB at 3.511 kb on + strand, within thrB at 3.607 kb on + strand, within thrB at 3.634 kb on - strand, within thrB at 3.638 kb on + strand, within thrB at 3.638 kb on + strand, within thrB at 3.650 kb on + strand at 3.769 kb on + strand at 3.769 kb on + strand at 3.792 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.25mM remove 70 + +0.5 70 + +0.9 78 - -1.6 78 - -1.9 161 - -4.6 185 - -1.4 185 - -1.4 208 + thrL b0001 0.27 +1.3 217 - thrL b0001 0.41 -4.0 257 - -0.7 257 - -4.0 440 - -4.2 440 - -4.0 454 - -3.9 518 - -2.8 518 - -3.5 528 + -3.2 592 + thrA b0002 0.10 -3.6 592 + thrA b0002 0.10 -1.5 666 + thrA b0002 0.13 -2.7 666 + thrA b0002 0.13 -2.7 675 - thrA b0002 0.14 -5.6 754 - thrA b0002 0.17 -5.2 754 - thrA b0002 0.17 +0.4 767 + thrA b0002 0.17 -3.6 775 - thrA b0002 0.18 -3.9 957 + thrA b0002 0.25 -4.0 957 + thrA b0002 0.25 -1.0 1,046 + thrA b0002 0.29 -3.7 1,136 - thrA b0002 0.32 -5.7 1,142 - thrA b0002 0.33 -5.4 1,161 + thrA b0002 0.33 -3.5 1,161 + thrA b0002 0.33 -4.3 1,161 + thrA b0002 0.33 -3.6 1,259 - thrA b0002 0.37 -5.0 1,259 - thrA b0002 0.37 -4.7 1,262 + thrA b0002 0.38 -6.0 1,270 - thrA b0002 0.38 -3.0 1,270 - thrA b0002 0.38 -3.1 1,280 - thrA b0002 0.38 -3.7 1,489 + thrA b0002 0.47 -0.8 1,599 - thrA b0002 0.51 -3.3 1,606 + thrA b0002 0.52 -4.8 1,627 + thrA b0002 0.52 -4.3 1,633 - thrA b0002 0.53 -3.6 1,633 - thrA b0002 0.53 -4.2 1,636 + thrA b0002 0.53 -3.6 1,636 + thrA b0002 0.53 -2.8 1,637 + thrA b0002 0.53 -5.0 1,711 - thrA b0002 0.56 -2.8 1,721 - thrA b0002 0.56 -3.6 1,767 - thrA b0002 0.58 -4.8 1,793 - thrA b0002 0.59 -6.0 1,868 + thrA b0002 0.62 -3.2 1,868 + thrA b0002 0.62 -4.3 1,871 + thrA b0002 0.62 -3.8 1,876 - thrA b0002 0.62 -2.9 1,881 - thrA b0002 0.63 -4.0 1,881 - thrA b0002 0.63 -3.2 1,929 + thrA b0002 0.65 -0.9 1,929 + thrA b0002 0.65 -3.4 1,937 - thrA b0002 0.65 -0.3 1,937 - thrA b0002 0.65 -0.6 2,076 + thrA b0002 0.71 -7.0 2,104 + thrA b0002 0.72 -3.7 2,104 + thrA b0002 0.72 -1.1 2,266 - thrA b0002 0.78 -2.9 2,266 - thrA b0002 0.78 -2.9 2,292 - thrA b0002 0.79 -4.0 2,294 + thrA b0002 0.79 -4.7 2,370 - thrA b0002 0.83 -3.1 2,370 - thrA b0002 0.83 -1.9 2,449 - thrA b0002 0.86 -4.7 2,590 - -3.3 2,675 + -2.4 2,682 + -4.0 2,682 + +0.7 2,684 + -6.0 2,717 + -4.6 2,744 - -4.7 2,787 - -3.1 2,787 - -2.9 2,829 + -4.8 2,847 + -2.5 2,847 + -2.8 2,906 - thrB b0003 0.11 -3.0 2,934 + thrB b0003 0.14 -5.9 2,963 - thrB b0003 0.17 -4.4 3,020 - thrB b0003 0.23 -3.5 3,023 + thrB b0003 0.24 -4.0 3,131 + thrB b0003 0.35 -6.0 3,144 - thrB b0003 0.37 -3.3 3,186 + thrB b0003 0.41 -4.8 3,246 + thrB b0003 0.48 -3.4 3,248 + thrB b0003 0.48 -5.0 3,256 + thrB b0003 0.49 -5.5 3,293 + thrB b0003 0.53 -0.6 3,301 - thrB b0003 0.54 -2.2 3,318 - thrB b0003 0.55 -5.3 3,411 + thrB b0003 0.65 -4.1 3,444 - thrB b0003 0.69 -4.1 3,444 - thrB b0003 0.69 -1.2 3,504 - thrB b0003 0.75 -4.9 3,504 - thrB b0003 0.75 -2.7 3,509 - thrB b0003 0.76 -1.6 3,511 + thrB b0003 0.76 -3.4 3,607 + thrB b0003 0.86 -3.9 3,634 - thrB b0003 0.89 -2.1 3,638 + thrB b0003 0.90 -4.5 3,638 + thrB b0003 0.90 -3.5 3,650 + -2.8 3,769 + -2.8 3,769 + -1.1 3,792 - -4.1
Or see this region's nucleotide sequence