Strain Fitness in Escherichia coli BW25113 around b4512

Experiment: carbon source D-Glucose 0.25mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcstA and ybdD are separated by 182 nucleotidesybdD and ybdH are separated by 9 nucleotides b0598: cstA - carbon starvation protein (NCBI), at 629,117 to 631,222 cstA b4512: ybdD - hypothetical protein (NCBI), at 631,405 to 631,602 ybdD b0599: ybdH - predicted oxidoreductase (NCBI), at 631,612 to 632,700 ybdH Position (kb) 631 632Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 630.485 kb on + strand, within cstAat 630.486 kb on - strand, within cstAat 630.493 kb on - strand, within cstAat 630.511 kb on + strand, within cstAat 630.583 kb on + strand, within cstAat 630.640 kb on + strand, within cstAat 630.703 kb on + strand, within cstAat 630.766 kb on + strand, within cstAat 630.816 kb on + strand, within cstAat 630.834 kb on - strand, within cstAat 630.834 kb on - strand, within cstAat 630.853 kb on - strand, within cstAat 630.865 kb on + strand, within cstAat 630.865 kb on + strand, within cstAat 630.894 kb on - strand, within cstAat 630.927 kb on - strand, within cstAat 630.927 kb on - strand, within cstAat 631.000 kb on + strand, within cstAat 631.000 kb on + strand, within cstAat 631.003 kb on + strand, within cstAat 631.068 kb on + strandat 631.068 kb on + strandat 631.126 kb on + strandat 631.180 kb on - strandat 631.194 kb on + strandat 631.194 kb on + strandat 631.236 kb on - strandat 631.250 kb on + strandat 631.262 kb on + strandat 631.262 kb on + strandat 631.264 kb on + strandat 631.278 kb on - strandat 631.300 kb on - strandat 631.300 kb on - strandat 631.305 kb on + strandat 631.398 kb on + strandat 631.398 kb on + strandat 631.401 kb on - strandat 631.413 kb on - strandat 631.413 kb on - strandat 631.780 kb on - strand, within ybdHat 631.782 kb on + strand, within ybdHat 631.782 kb on + strand, within ybdHat 631.825 kb on + strand, within ybdHat 631.828 kb on - strand, within ybdHat 631.828 kb on - strand, within ybdHat 631.833 kb on - strand, within ybdHat 631.833 kb on - strand, within ybdHat 631.926 kb on + strand, within ybdHat 631.926 kb on - strand, within ybdHat 632.035 kb on + strand, within ybdHat 632.048 kb on + strand, within ybdHat 632.147 kb on + strand, within ybdHat 632.160 kb on + strand, within ybdHat 632.399 kb on + strand, within ybdHat 632.466 kb on - strand, within ybdHat 632.581 kb on + strand, within ybdH

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.25mM
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630,485 + cstA b0598 0.65 -0.1
630,486 - cstA b0598 0.65 +1.2
630,493 - cstA b0598 0.65 -2.3
630,511 + cstA b0598 0.66 -3.5
630,583 + cstA b0598 0.70 +0.7
630,640 + cstA b0598 0.72 -4.1
630,703 + cstA b0598 0.75 +0.1
630,766 + cstA b0598 0.78 +1.0
630,816 + cstA b0598 0.81 +0.9
630,834 - cstA b0598 0.82 +1.3
630,834 - cstA b0598 0.82 +0.5
630,853 - cstA b0598 0.82 +0.7
630,865 + cstA b0598 0.83 +0.7
630,865 + cstA b0598 0.83 -0.0
630,894 - cstA b0598 0.84 +2.3
630,927 - cstA b0598 0.86 +1.4
630,927 - cstA b0598 0.86 +2.0
631,000 + cstA b0598 0.89 -4.2
631,000 + cstA b0598 0.89 +1.2
631,003 + cstA b0598 0.90 +1.7
631,068 + +0.2
631,068 + -0.2
631,126 + -0.7
631,180 - +0.9
631,194 + +1.7
631,194 + -4.2
631,236 - +0.7
631,250 + +1.6
631,262 + +1.8
631,262 + -2.9
631,264 + -0.9
631,278 - -0.2
631,300 - +1.0
631,300 - +1.3
631,305 + -0.3
631,398 + +1.7
631,398 + -2.7
631,401 - -2.4
631,413 - -0.1
631,413 - +0.5
631,780 - ybdH b0599 0.15 +2.3
631,782 + ybdH b0599 0.16 +0.0
631,782 + ybdH b0599 0.16 -4.1
631,825 + ybdH b0599 0.20 +1.1
631,828 - ybdH b0599 0.20 +0.5
631,828 - ybdH b0599 0.20 -0.4
631,833 - ybdH b0599 0.20 +0.1
631,833 - ybdH b0599 0.20 +2.4
631,926 + ybdH b0599 0.29 -3.0
631,926 - ybdH b0599 0.29 -3.4
632,035 + ybdH b0599 0.39 -1.9
632,048 + ybdH b0599 0.40 +0.0
632,147 + ybdH b0599 0.49 -3.6
632,160 + ybdH b0599 0.50 +0.3
632,399 + ybdH b0599 0.72 +1.1
632,466 - ybdH b0599 0.78 +1.4
632,581 + ybdH b0599 0.89 +0.8

Or see this region's nucleotide sequence