Strain Fitness in Escherichia coli BW25113 around b4227

Experiment: carbon source D-Glucose 0.25mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntchpB and ppa are separated by 79 nucleotidesppa and ytfQ are separated by 309 nucleotidesytfQ and ytfR are separated by 139 nucleotides b4225: chpB - toxin of the ChpB-ChpS toxin-antitoxin system (NCBI), at 4,446,715 to 4,447,065 chpB b4226: ppa - inorganic pyrophosphatase (NCBI), at 4,447,145 to 4,447,675 ppa b4227: ytfQ - predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 4,447,985 to 4,448,941 ytfQ b4485: ytfR - predicted sugar transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 4,449,081 to 4,450,583 ytfR Position (kb) 4447 4448 4449Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4447.006 kb on - strand, within chpBat 4447.006 kb on - strand, within chpBat 4447.057 kb on + strandat 4447.060 kb on + strandat 4447.060 kb on + strandat 4447.779 kb on + strandat 4447.787 kb on + strandat 4447.792 kb on + strandat 4447.795 kb on - strandat 4447.795 kb on - strandat 4447.797 kb on + strandat 4447.970 kb on + strandat 4447.978 kb on - strandat 4447.983 kb on - strandat 4447.983 kb on - strandat 4448.010 kb on + strandat 4448.039 kb on + strandat 4448.039 kb on - strandat 4448.345 kb on + strand, within ytfQat 4448.347 kb on + strand, within ytfQat 4448.429 kb on + strand, within ytfQat 4448.429 kb on + strand, within ytfQat 4448.452 kb on + strand, within ytfQat 4448.538 kb on + strand, within ytfQat 4448.630 kb on + strand, within ytfQat 4448.630 kb on + strand, within ytfQat 4448.630 kb on + strand, within ytfQat 4448.630 kb on + strand, within ytfQat 4448.653 kb on + strand, within ytfQat 4448.675 kb on + strand, within ytfQat 4448.732 kb on + strand, within ytfQat 4448.770 kb on - strand, within ytfQat 4448.825 kb on + strand, within ytfQat 4448.825 kb on + strand, within ytfQat 4448.884 kb on + strandat 4448.888 kb on - strandat 4448.890 kb on + strandat 4449.060 kb on + strandat 4449.060 kb on + strandat 4449.062 kb on + strandat 4449.062 kb on + strandat 4449.069 kb on - strandat 4449.249 kb on + strand, within ytfRat 4449.249 kb on + strand, within ytfRat 4449.257 kb on - strand, within ytfRat 4449.593 kb on + strand, within ytfRat 4449.705 kb on + strand, within ytfRat 4449.718 kb on - strand, within ytfR

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.25mM
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4,447,006 - chpB b4225 0.83 -3.2
4,447,006 - chpB b4225 0.83 -0.6
4,447,057 + -2.7
4,447,060 + +1.5
4,447,060 + -3.0
4,447,779 + -3.1
4,447,787 + +0.9
4,447,792 + -3.6
4,447,795 - +0.5
4,447,795 - +0.1
4,447,797 + -3.4
4,447,970 + +0.1
4,447,978 - +0.9
4,447,983 - -2.4
4,447,983 - +1.9
4,448,010 + +0.4
4,448,039 + -2.3
4,448,039 - -2.4
4,448,345 + ytfQ b4227 0.38 -3.3
4,448,347 + ytfQ b4227 0.38 -2.1
4,448,429 + ytfQ b4227 0.46 +0.3
4,448,429 + ytfQ b4227 0.46 -1.8
4,448,452 + ytfQ b4227 0.49 -2.1
4,448,538 + ytfQ b4227 0.58 +0.2
4,448,630 + ytfQ b4227 0.67 +1.6
4,448,630 + ytfQ b4227 0.67 -2.2
4,448,630 + ytfQ b4227 0.67 +1.4
4,448,630 + ytfQ b4227 0.67 -3.1
4,448,653 + ytfQ b4227 0.70 -3.9
4,448,675 + ytfQ b4227 0.72 -4.3
4,448,732 + ytfQ b4227 0.78 +0.2
4,448,770 - ytfQ b4227 0.82 +0.0
4,448,825 + ytfQ b4227 0.88 -2.2
4,448,825 + ytfQ b4227 0.88 -2.4
4,448,884 + +0.1
4,448,888 - +0.4
4,448,890 + -0.6
4,449,060 + +0.4
4,449,060 + +0.5
4,449,062 + -0.1
4,449,062 + -3.6
4,449,069 - +1.8
4,449,249 + ytfR b4485 0.11 -3.5
4,449,249 + ytfR b4485 0.11 -3.9
4,449,257 - ytfR b4485 0.12 +1.6
4,449,593 + ytfR b4485 0.34 +0.3
4,449,705 + ytfR b4485 0.42 -3.2
4,449,718 - ytfR b4485 0.42 +0.7

Or see this region's nucleotide sequence