Experiment: carbon source D-Glucose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhfU and php are separated by 10 nucleotides php and yhfW overlap by 4 nucleotides yhfW and yhfX overlap by 1 nucleotides
b3378: yhfU - orf, hypothetical protein (VIMSS), at 3,505,370 to 3,505,723
yhfU
b3379: php - predicted hydrolase (NCBI), at 3,505,734 to 3,506,612
php
b3380: yhfW - predicted mutase (NCBI), at 3,506,609 to 3,507,835
yhfW
b3381: yhfX - predicted amino acid racemase (NCBI), at 3,507,835 to 3,508,998
yhfX
Position (kb)
3506
3507
3508 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3505.652 kb on + strand, within yhfU at 3505.652 kb on + strand, within yhfU at 3505.659 kb on - strand, within yhfU at 3505.687 kb on + strand, within yhfU at 3505.718 kb on + strand at 3505.724 kb on + strand at 3505.725 kb on + strand at 3505.734 kb on - strand at 3505.767 kb on + strand at 3505.767 kb on + strand at 3505.780 kb on - strand at 3505.787 kb on + strand at 3505.793 kb on + strand at 3505.793 kb on + strand at 3505.796 kb on - strand at 3505.895 kb on + strand, within php at 3505.913 kb on - strand, within php at 3505.992 kb on - strand, within php at 3506.060 kb on - strand, within php at 3506.060 kb on - strand, within php at 3506.062 kb on + strand, within php at 3506.165 kb on + strand, within php at 3506.168 kb on - strand, within php at 3506.168 kb on - strand, within php at 3506.173 kb on - strand, within php at 3506.238 kb on - strand, within php at 3506.296 kb on + strand, within php at 3506.296 kb on + strand, within php at 3506.296 kb on + strand, within php at 3506.313 kb on + strand, within php at 3506.313 kb on + strand, within php at 3506.320 kb on - strand, within php at 3506.330 kb on + strand, within php at 3506.371 kb on + strand, within php at 3506.379 kb on - strand, within php at 3506.379 kb on - strand, within php at 3506.385 kb on + strand, within php at 3506.458 kb on + strand, within php at 3506.458 kb on + strand, within php at 3506.469 kb on - strand, within php at 3506.489 kb on - strand, within php at 3506.489 kb on - strand, within php at 3506.509 kb on + strand, within php at 3506.558 kb on + strand at 3506.584 kb on - strand at 3506.594 kb on - strand at 3506.595 kb on - strand at 3506.697 kb on + strand at 3506.703 kb on + strand at 3506.707 kb on - strand at 3506.707 kb on - strand at 3506.715 kb on + strand at 3506.715 kb on + strand at 3506.734 kb on + strand, within yhfW at 3506.734 kb on + strand, within yhfW at 3506.772 kb on - strand, within yhfW at 3506.792 kb on + strand, within yhfW at 3506.792 kb on + strand, within yhfW at 3506.813 kb on - strand, within yhfW at 3506.813 kb on - strand, within yhfW at 3506.858 kb on + strand, within yhfW at 3506.885 kb on - strand, within yhfW at 3506.950 kb on + strand, within yhfW at 3506.976 kb on + strand, within yhfW at 3507.017 kb on - strand, within yhfW at 3507.017 kb on - strand, within yhfW at 3507.078 kb on + strand, within yhfW at 3507.078 kb on + strand, within yhfW at 3507.089 kb on - strand, within yhfW at 3507.089 kb on - strand, within yhfW at 3507.094 kb on + strand, within yhfW at 3507.108 kb on - strand, within yhfW at 3507.155 kb on + strand, within yhfW at 3507.155 kb on + strand, within yhfW at 3507.157 kb on + strand, within yhfW at 3507.169 kb on + strand, within yhfW at 3507.169 kb on + strand, within yhfW at 3507.189 kb on + strand, within yhfW at 3507.205 kb on + strand, within yhfW at 3507.213 kb on - strand, within yhfW at 3507.242 kb on + strand, within yhfW at 3507.242 kb on + strand, within yhfW at 3507.274 kb on - strand, within yhfW at 3507.286 kb on - strand, within yhfW at 3507.300 kb on + strand, within yhfW at 3507.300 kb on + strand, within yhfW at 3507.300 kb on + strand, within yhfW at 3507.308 kb on - strand, within yhfW at 3507.308 kb on - strand, within yhfW at 3507.310 kb on + strand, within yhfW at 3507.327 kb on - strand, within yhfW at 3507.343 kb on + strand, within yhfW at 3507.386 kb on - strand, within yhfW at 3507.459 kb on - strand, within yhfW at 3507.472 kb on - strand, within yhfW at 3507.472 kb on - strand, within yhfW at 3507.525 kb on - strand, within yhfW at 3507.525 kb on - strand, within yhfW at 3507.574 kb on + strand, within yhfW at 3507.574 kb on + strand, within yhfW at 3507.607 kb on + strand, within yhfW at 3507.607 kb on + strand, within yhfW at 3507.664 kb on - strand, within yhfW at 3507.664 kb on - strand, within yhfW at 3507.688 kb on + strand, within yhfW at 3507.694 kb on + strand, within yhfW at 3507.710 kb on - strand, within yhfW at 3507.727 kb on + strand at 3507.741 kb on - strand at 3507.743 kb on + strand at 3507.743 kb on + strand at 3507.746 kb on - strand at 3507.746 kb on - strand at 3507.767 kb on + strand at 3507.767 kb on + strand at 3507.767 kb on + strand at 3507.796 kb on + strand at 3507.870 kb on - strand at 3507.899 kb on + strand at 3507.918 kb on - strand at 3507.955 kb on - strand, within yhfX at 3507.955 kb on - strand, within yhfX at 3507.960 kb on + strand, within yhfX at 3507.960 kb on + strand, within yhfX at 3507.960 kb on + strand, within yhfX at 3507.968 kb on - strand, within yhfX at 3507.968 kb on - strand, within yhfX at 3507.997 kb on - strand, within yhfX at 3508.001 kb on + strand, within yhfX at 3508.001 kb on + strand, within yhfX at 3508.013 kb on + strand, within yhfX at 3508.013 kb on - strand, within yhfX at 3508.013 kb on - strand, within yhfX at 3508.018 kb on - strand, within yhfX at 3508.101 kb on - strand, within yhfX at 3508.110 kb on - strand, within yhfX at 3508.178 kb on + strand, within yhfX at 3508.185 kb on + strand, within yhfX at 3508.255 kb on + strand, within yhfX at 3508.263 kb on - strand, within yhfX at 3508.369 kb on - strand, within yhfX at 3508.486 kb on + strand, within yhfX at 3508.563 kb on + strand, within yhfX at 3508.567 kb on - strand, within yhfX at 3508.634 kb on + strand, within yhfX at 3508.634 kb on + strand, within yhfX at 3508.650 kb on - strand, within yhfX at 3508.652 kb on - strand, within yhfX at 3508.675 kb on - strand, within yhfX at 3508.675 kb on - strand, within yhfX at 3508.685 kb on + strand, within yhfX at 3508.823 kb on + strand, within yhfX at 3508.831 kb on - strand, within yhfX at 3508.833 kb on + strand, within yhfX
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.25mM remove 3,505,652 + yhfU b3378 0.80 -4.1 3,505,652 + yhfU b3378 0.80 -1.6 3,505,659 - yhfU b3378 0.82 +0.3 3,505,687 + yhfU b3378 0.90 +1.7 3,505,718 + +3.1 3,505,724 + -1.4 3,505,725 + +0.1 3,505,734 - +1.0 3,505,767 + +0.3 3,505,767 + -2.9 3,505,780 - +0.3 3,505,787 + +0.6 3,505,793 + -0.7 3,505,793 + -0.4 3,505,796 - +1.9 3,505,895 + php b3379 0.18 +3.4 3,505,913 - php b3379 0.20 -3.9 3,505,992 - php b3379 0.29 +0.4 3,506,060 - php b3379 0.37 -4.2 3,506,060 - php b3379 0.37 +2.4 3,506,062 + php b3379 0.37 +0.9 3,506,165 + php b3379 0.49 +1.4 3,506,168 - php b3379 0.49 +1.5 3,506,168 - php b3379 0.49 -2.1 3,506,173 - php b3379 0.50 +0.3 3,506,238 - php b3379 0.57 +0.4 3,506,296 + php b3379 0.64 +0.5 3,506,296 + php b3379 0.64 -1.9 3,506,296 + php b3379 0.64 +1.9 3,506,313 + php b3379 0.66 +0.4 3,506,313 + php b3379 0.66 +2.1 3,506,320 - php b3379 0.67 -0.5 3,506,330 + php b3379 0.68 +1.3 3,506,371 + php b3379 0.72 +1.5 3,506,379 - php b3379 0.73 +1.3 3,506,379 - php b3379 0.73 -3.5 3,506,385 + php b3379 0.74 +0.9 3,506,458 + php b3379 0.82 +0.9 3,506,458 + php b3379 0.82 -3.5 3,506,469 - php b3379 0.84 +2.1 3,506,489 - php b3379 0.86 -2.4 3,506,489 - php b3379 0.86 +2.0 3,506,509 + php b3379 0.88 +1.7 3,506,558 + -4.7 3,506,584 - +0.0 3,506,594 - -2.6 3,506,595 - +1.2 3,506,697 + -1.4 3,506,703 + -1.4 3,506,707 - +1.2 3,506,707 - -3.8 3,506,715 + -3.3 3,506,715 + -3.7 3,506,734 + yhfW b3380 0.10 -4.0 3,506,734 + yhfW b3380 0.10 -0.5 3,506,772 - yhfW b3380 0.13 +0.2 3,506,792 + yhfW b3380 0.15 +0.6 3,506,792 + yhfW b3380 0.15 -0.2 3,506,813 - yhfW b3380 0.17 +0.8 3,506,813 - yhfW b3380 0.17 +1.2 3,506,858 + yhfW b3380 0.20 +3.2 3,506,885 - yhfW b3380 0.22 +0.8 3,506,950 + yhfW b3380 0.28 +1.1 3,506,976 + yhfW b3380 0.30 +0.7 3,507,017 - yhfW b3380 0.33 +1.7 3,507,017 - yhfW b3380 0.33 -3.5 3,507,078 + yhfW b3380 0.38 +0.6 3,507,078 + yhfW b3380 0.38 +0.4 3,507,089 - yhfW b3380 0.39 -1.5 3,507,089 - yhfW b3380 0.39 -1.9 3,507,094 + yhfW b3380 0.40 -3.1 3,507,108 - yhfW b3380 0.41 -0.4 3,507,155 + yhfW b3380 0.44 -3.3 3,507,155 + yhfW b3380 0.44 +1.3 3,507,157 + yhfW b3380 0.45 +0.2 3,507,169 + yhfW b3380 0.46 +1.1 3,507,169 + yhfW b3380 0.46 +0.4 3,507,189 + yhfW b3380 0.47 +1.4 3,507,205 + yhfW b3380 0.49 -3.8 3,507,213 - yhfW b3380 0.49 -0.2 3,507,242 + yhfW b3380 0.52 -0.6 3,507,242 + yhfW b3380 0.52 +2.6 3,507,274 - yhfW b3380 0.54 +0.1 3,507,286 - yhfW b3380 0.55 -4.3 3,507,300 + yhfW b3380 0.56 +0.5 3,507,300 + yhfW b3380 0.56 -0.2 3,507,300 + yhfW b3380 0.56 -0.6 3,507,308 - yhfW b3380 0.57 +0.2 3,507,308 - yhfW b3380 0.57 -0.1 3,507,310 + yhfW b3380 0.57 +2.9 3,507,327 - yhfW b3380 0.59 +0.7 3,507,343 + yhfW b3380 0.60 -2.9 3,507,386 - yhfW b3380 0.63 -1.3 3,507,459 - yhfW b3380 0.69 +0.1 3,507,472 - yhfW b3380 0.70 -0.1 3,507,472 - yhfW b3380 0.70 +1.0 3,507,525 - yhfW b3380 0.75 -2.1 3,507,525 - yhfW b3380 0.75 -3.5 3,507,574 + yhfW b3380 0.79 +0.8 3,507,574 + yhfW b3380 0.79 +0.6 3,507,607 + yhfW b3380 0.81 +0.2 3,507,607 + yhfW b3380 0.81 -2.7 3,507,664 - yhfW b3380 0.86 +0.3 3,507,664 - yhfW b3380 0.86 +0.5 3,507,688 + yhfW b3380 0.88 +1.1 3,507,694 + yhfW b3380 0.88 -1.9 3,507,710 - yhfW b3380 0.90 -0.7 3,507,727 + -4.6 3,507,741 - +1.0 3,507,743 + -0.0 3,507,743 + -0.0 3,507,746 - -2.7 3,507,746 - -1.4 3,507,767 + +0.8 3,507,767 + -1.0 3,507,767 + +0.5 3,507,796 + +1.1 3,507,870 - +0.7 3,507,899 + -0.2 3,507,918 - +2.0 3,507,955 - yhfX b3381 0.10 +0.1 3,507,955 - yhfX b3381 0.10 -4.7 3,507,960 + yhfX b3381 0.11 +0.2 3,507,960 + yhfX b3381 0.11 -2.1 3,507,960 + yhfX b3381 0.11 +0.7 3,507,968 - yhfX b3381 0.11 -5.3 3,507,968 - yhfX b3381 0.11 -4.5 3,507,997 - yhfX b3381 0.14 +0.8 3,508,001 + yhfX b3381 0.14 +1.7 3,508,001 + yhfX b3381 0.14 -3.0 3,508,013 + yhfX b3381 0.15 -0.1 3,508,013 - yhfX b3381 0.15 -4.1 3,508,013 - yhfX b3381 0.15 -4.5 3,508,018 - yhfX b3381 0.16 -4.1 3,508,101 - yhfX b3381 0.23 +1.9 3,508,110 - yhfX b3381 0.24 -0.6 3,508,178 + yhfX b3381 0.29 +0.7 3,508,185 + yhfX b3381 0.30 +0.6 3,508,255 + yhfX b3381 0.36 -3.9 3,508,263 - yhfX b3381 0.37 -4.1 3,508,369 - yhfX b3381 0.46 +0.3 3,508,486 + yhfX b3381 0.56 -4.1 3,508,563 + yhfX b3381 0.63 +0.8 3,508,567 - yhfX b3381 0.63 -1.0 3,508,634 + yhfX b3381 0.69 +1.6 3,508,634 + yhfX b3381 0.69 -3.4 3,508,650 - yhfX b3381 0.70 -1.2 3,508,652 - yhfX b3381 0.70 +0.3 3,508,675 - yhfX b3381 0.72 +1.3 3,508,675 - yhfX b3381 0.72 +0.3 3,508,685 + yhfX b3381 0.73 -3.7 3,508,823 + yhfX b3381 0.85 -2.8 3,508,831 - yhfX b3381 0.86 +0.4 3,508,833 + yhfX b3381 0.86 +1.0
Or see this region's nucleotide sequence