Experiment: carbon source D-Glucose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hcaD and yphA are separated by 96 nucleotides yphA and yphB are separated by 47 nucleotides yphB and yphC are separated by 11 nucleotides
b2542: hcaD - phenylpropionate dioxygenase, ferredoxin reductase subunit (NCBI), at 2,670,069 to 2,671,271
hcaD
b2543: yphA - predicted inner membrane protein (RefSeq), at 2,671,368 to 2,671,790
yphA
b2544: yphB - hypothetical protein (NCBI), at 2,671,838 to 2,672,710
yphB
b2545: yphC - putative oxidoreductase (VIMSS), at 2,672,722 to 2,673,783
yphC
Position (kb)
2671
2672 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2670.427 kb on - strand, within hcaD at 2670.427 kb on - strand, within hcaD at 2670.447 kb on + strand, within hcaD at 2670.450 kb on - strand, within hcaD at 2670.450 kb on - strand, within hcaD at 2670.576 kb on - strand, within hcaD at 2670.576 kb on - strand, within hcaD at 2670.576 kb on - strand, within hcaD at 2670.603 kb on + strand, within hcaD at 2670.836 kb on - strand, within hcaD at 2670.927 kb on - strand, within hcaD at 2670.994 kb on - strand, within hcaD at 2670.994 kb on - strand, within hcaD at 2671.037 kb on + strand, within hcaD at 2671.203 kb on + strand at 2671.315 kb on + strand at 2671.315 kb on + strand at 2671.315 kb on + strand at 2671.323 kb on - strand at 2671.373 kb on - strand at 2671.373 kb on - strand at 2671.494 kb on + strand, within yphA at 2671.494 kb on + strand, within yphA at 2671.496 kb on + strand, within yphA at 2671.497 kb on - strand, within yphA at 2671.502 kb on - strand, within yphA at 2671.502 kb on - strand, within yphA at 2671.509 kb on + strand, within yphA at 2671.603 kb on + strand, within yphA at 2671.641 kb on - strand, within yphA at 2671.645 kb on - strand, within yphA at 2671.713 kb on + strand, within yphA at 2671.736 kb on + strand, within yphA at 2671.736 kb on + strand, within yphA at 2671.789 kb on + strand at 2671.890 kb on - strand at 2671.959 kb on - strand, within yphB at 2672.031 kb on - strand, within yphB at 2672.031 kb on - strand, within yphB at 2672.053 kb on + strand, within yphB at 2672.081 kb on + strand, within yphB at 2672.084 kb on - strand, within yphB at 2672.165 kb on - strand, within yphB at 2672.165 kb on - strand, within yphB at 2672.380 kb on - strand, within yphB at 2672.394 kb on + strand, within yphB at 2672.394 kb on + strand, within yphB at 2672.423 kb on + strand, within yphB at 2672.471 kb on - strand, within yphB at 2672.471 kb on - strand, within yphB at 2672.690 kb on + strand at 2672.697 kb on + strand at 2672.703 kb on - strand at 2672.704 kb on - strand at 2672.705 kb on - strand at 2672.764 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.25mM remove 2,670,427 - hcaD b2542 0.30 +0.1 2,670,427 - hcaD b2542 0.30 +1.6 2,670,447 + hcaD b2542 0.31 +0.5 2,670,450 - hcaD b2542 0.32 +0.7 2,670,450 - hcaD b2542 0.32 +1.1 2,670,576 - hcaD b2542 0.42 -4.4 2,670,576 - hcaD b2542 0.42 +1.2 2,670,576 - hcaD b2542 0.42 +1.1 2,670,603 + hcaD b2542 0.44 +0.2 2,670,836 - hcaD b2542 0.64 +0.6 2,670,927 - hcaD b2542 0.71 -3.9 2,670,994 - hcaD b2542 0.77 -3.1 2,670,994 - hcaD b2542 0.77 +1.2 2,671,037 + hcaD b2542 0.80 +1.5 2,671,203 + -2.3 2,671,315 + -1.0 2,671,315 + +0.1 2,671,315 + +0.7 2,671,323 - +2.6 2,671,373 - +2.6 2,671,373 - +0.3 2,671,494 + yphA b2543 0.30 +1.4 2,671,494 + yphA b2543 0.30 -3.6 2,671,496 + yphA b2543 0.30 -4.2 2,671,497 - yphA b2543 0.30 +0.2 2,671,502 - yphA b2543 0.32 +2.8 2,671,502 - yphA b2543 0.32 -2.5 2,671,509 + yphA b2543 0.33 +1.7 2,671,603 + yphA b2543 0.56 +0.7 2,671,641 - yphA b2543 0.65 -4.1 2,671,645 - yphA b2543 0.65 -0.7 2,671,713 + yphA b2543 0.82 +1.9 2,671,736 + yphA b2543 0.87 -0.1 2,671,736 + yphA b2543 0.87 -3.7 2,671,789 + +0.1 2,671,890 - -0.6 2,671,959 - yphB b2544 0.14 +0.1 2,672,031 - yphB b2544 0.22 +1.9 2,672,031 - yphB b2544 0.22 +0.7 2,672,053 + yphB b2544 0.25 -1.4 2,672,081 + yphB b2544 0.28 -0.2 2,672,084 - yphB b2544 0.28 -0.0 2,672,165 - yphB b2544 0.37 -0.7 2,672,165 - yphB b2544 0.37 +1.8 2,672,380 - yphB b2544 0.62 -0.8 2,672,394 + yphB b2544 0.64 -2.9 2,672,394 + yphB b2544 0.64 -2.7 2,672,423 + yphB b2544 0.67 -0.8 2,672,471 - yphB b2544 0.73 -2.2 2,672,471 - yphB b2544 0.73 -2.4 2,672,690 + -1.0 2,672,697 + -4.2 2,672,703 - +0.4 2,672,704 - -0.7 2,672,705 - -2.9 2,672,764 + +1.9
Or see this region's nucleotide sequence