Strain Fitness in Escherichia coli BW25113 around b3188

Experiment: carbon source D-Glucose 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntispB and sfsB are separated by 227 nucleotidessfsB and murA are separated by 47 nucleotides b3187: ispB - octaprenyl diphosphate synthase (NCBI), at 3,331,732 to 3,332,703 ispB b3188: sfsB - DNA-binding transcriptional activator of maltose metabolism (NCBI), at 3,332,931 to 3,333,209 sfsB b3189: murA - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (NCBI), at 3,333,257 to 3,334,516 murA Position (kb) 3332 3333 3334Strain fitness (log2 ratio) -1 0 1 2at 3332.691 kb on - strandat 3332.718 kb on - strandat 3332.858 kb on - strandat 3332.858 kb on - strandat 3332.866 kb on + strandat 3332.913 kb on + strandat 3332.919 kb on - strandat 3333.104 kb on - strand, within sfsBat 3333.104 kb on - strand, within sfsBat 3333.144 kb on + strand, within sfsBat 3333.150 kb on + strand, within sfsBat 3333.151 kb on + strand, within sfsBat 3333.176 kb on + strand, within sfsBat 3333.190 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.5mM
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3,332,691 - -0.5
3,332,718 - -0.4
3,332,858 - +0.9
3,332,858 - +0.6
3,332,866 + -0.4
3,332,913 + -0.8
3,332,919 - +0.8
3,333,104 - sfsB b3188 0.62 +0.1
3,333,104 - sfsB b3188 0.62 +0.4
3,333,144 + sfsB b3188 0.76 +0.8
3,333,150 + sfsB b3188 0.78 -0.2
3,333,151 + sfsB b3188 0.79 +1.0
3,333,176 + sfsB b3188 0.88 +0.6
3,333,190 - +1.9

Or see this region's nucleotide sequence