Strain Fitness in Escherichia coli BW25113 around b2550

Experiment: carbon source D-Glucose 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyphG and yphH are separated by 117 nucleotidesyphH and glyA are separated by 197 nucleotides b2549: yphG - orf, hypothetical protein (VIMSS), at 2,677,486 to 2,680,767 yphG b2550: yphH - putative NAGC-like transcriptional regulator (VIMSS), at 2,680,885 to 2,682,078 yphH b2551: glyA - serine hydroxymethyltransferase (NCBI), at 2,682,276 to 2,683,529 glyA Position (kb) 2680 2681 2682 2683Strain fitness (log2 ratio) -2 -1 0 1 2at 2680.055 kb on + strand, within yphGat 2680.083 kb on + strand, within yphGat 2680.346 kb on - strand, within yphGat 2680.346 kb on - strand, within yphGat 2680.348 kb on + strand, within yphGat 2680.403 kb on - strand, within yphGat 2680.459 kb on - strandat 2680.459 kb on - strandat 2680.626 kb on - strandat 2680.626 kb on - strandat 2680.788 kb on + strandat 2681.096 kb on + strand, within yphHat 2681.096 kb on + strand, within yphHat 2681.099 kb on + strand, within yphHat 2681.176 kb on + strand, within yphHat 2681.316 kb on - strand, within yphHat 2681.433 kb on + strand, within yphHat 2681.433 kb on + strand, within yphHat 2681.440 kb on + strand, within yphHat 2681.612 kb on - strand, within yphHat 2681.629 kb on + strand, within yphHat 2681.726 kb on - strand, within yphHat 2681.771 kb on - strand, within yphHat 2681.938 kb on - strand, within yphHat 2681.938 kb on - strand, within yphHat 2682.052 kb on - strandat 2682.121 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.5mM
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2,680,055 + yphG b2549 0.78 -0.2
2,680,083 + yphG b2549 0.79 +0.2
2,680,346 - yphG b2549 0.87 -0.6
2,680,346 - yphG b2549 0.87 -0.1
2,680,348 + yphG b2549 0.87 -0.1
2,680,403 - yphG b2549 0.89 +0.2
2,680,459 - -1.0
2,680,459 - +0.6
2,680,626 - -0.6
2,680,626 - -0.1
2,680,788 + +0.4
2,681,096 + yphH b2550 0.18 +0.6
2,681,096 + yphH b2550 0.18 +0.4
2,681,099 + yphH b2550 0.18 -0.2
2,681,176 + yphH b2550 0.24 -0.3
2,681,316 - yphH b2550 0.36 +0.4
2,681,433 + yphH b2550 0.46 +2.4
2,681,433 + yphH b2550 0.46 +0.8
2,681,440 + yphH b2550 0.46 +0.8
2,681,612 - yphH b2550 0.61 +0.6
2,681,629 + yphH b2550 0.62 +0.2
2,681,726 - yphH b2550 0.70 -1.8
2,681,771 - yphH b2550 0.74 +0.7
2,681,938 - yphH b2550 0.88 -0.1
2,681,938 - yphH b2550 0.88 +0.6
2,682,052 - +1.0
2,682,121 - -0.3

Or see this region's nucleotide sequence