Strain Fitness in Escherichia coli BW25113 around b1923

Experiment: carbon source D-Glucose 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliA and fliC are separated by 320 nucleotidesfliC and fliD are separated by 265 nucleotides b1922: fliA - flagellar biosynthesis; alternative sigma factor 28; regulation of flagellar operons (VIMSS), at 1,999,094 to 1,999,813 fliA b1923: fliC - flagellin (NCBI), at 2,000,134 to 2,001,630 fliC b1924: fliD - flagellar capping protein (NCBI), at 2,001,896 to 2,003,302 fliD Position (kb) 2000 2001 2002Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1999.139 kb on + strandat 1999.145 kb on - strandat 1999.145 kb on - strandat 1999.177 kb on - strand, within fliAat 1999.209 kb on - strand, within fliAat 1999.292 kb on - strand, within fliAat 1999.292 kb on - strand, within fliAat 1999.302 kb on - strand, within fliAat 1999.504 kb on - strand, within fliAat 1999.504 kb on - strand, within fliAat 1999.508 kb on - strand, within fliAat 1999.509 kb on - strand, within fliAat 1999.509 kb on - strand, within fliAat 1999.541 kb on + strand, within fliAat 1999.595 kb on - strand, within fliAat 1999.600 kb on - strand, within fliAat 1999.615 kb on + strand, within fliAat 1999.655 kb on + strand, within fliAat 1999.800 kb on - strandat 1999.832 kb on + strandat 1999.903 kb on - strandat 1999.969 kb on + strandat 1999.969 kb on + strandat 2000.236 kb on - strandat 2000.439 kb on - strand, within fliCat 2000.523 kb on + strand, within fliCat 2000.591 kb on + strand, within fliCat 2000.591 kb on + strand, within fliCat 2000.766 kb on - strand, within fliCat 2000.772 kb on + strand, within fliCat 2000.772 kb on + strand, within fliCat 2000.849 kb on - strand, within fliCat 2000.935 kb on + strand, within fliCat 2000.935 kb on + strand, within fliCat 2000.954 kb on + strand, within fliCat 2000.981 kb on - strand, within fliCat 2001.074 kb on - strand, within fliCat 2001.074 kb on - strand, within fliCat 2001.083 kb on - strand, within fliCat 2001.083 kb on - strand, within fliCat 2001.090 kb on - strand, within fliCat 2001.090 kb on - strand, within fliCat 2001.329 kb on + strand, within fliCat 2001.337 kb on - strand, within fliCat 2001.337 kb on - strand, within fliCat 2001.400 kb on + strand, within fliCat 2001.499 kb on - strandat 2001.522 kb on - strandat 2001.522 kb on - strandat 2001.548 kb on + strandat 2001.548 kb on + strandat 2001.706 kb on + strandat 2001.706 kb on + strandat 2001.842 kb on - strandat 2001.923 kb on + strandat 2001.964 kb on - strandat 2002.119 kb on + strand, within fliDat 2002.119 kb on + strand, within fliDat 2002.190 kb on - strand, within fliDat 2002.353 kb on + strand, within fliDat 2002.456 kb on + strand, within fliDat 2002.456 kb on + strand, within fliDat 2002.547 kb on - strand, within fliD

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.5mM
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1,999,139 + -0.7
1,999,145 - +0.2
1,999,145 - +1.1
1,999,177 - fliA b1922 0.12 +0.8
1,999,209 - fliA b1922 0.16 +0.4
1,999,292 - fliA b1922 0.28 -0.5
1,999,292 - fliA b1922 0.28 +0.1
1,999,302 - fliA b1922 0.29 +0.9
1,999,504 - fliA b1922 0.57 +0.4
1,999,504 - fliA b1922 0.57 -0.5
1,999,508 - fliA b1922 0.57 -0.7
1,999,509 - fliA b1922 0.58 -1.4
1,999,509 - fliA b1922 0.58 -0.7
1,999,541 + fliA b1922 0.62 -0.8
1,999,595 - fliA b1922 0.70 -0.2
1,999,600 - fliA b1922 0.70 +0.3
1,999,615 + fliA b1922 0.72 -0.4
1,999,655 + fliA b1922 0.78 +0.1
1,999,800 - +0.8
1,999,832 + +1.4
1,999,903 - -2.5
1,999,969 + -0.0
1,999,969 + +0.2
2,000,236 - +0.3
2,000,439 - fliC b1923 0.20 +1.2
2,000,523 + fliC b1923 0.26 -0.1
2,000,591 + fliC b1923 0.31 +0.8
2,000,591 + fliC b1923 0.31 +1.4
2,000,766 - fliC b1923 0.42 +0.1
2,000,772 + fliC b1923 0.43 +0.0
2,000,772 + fliC b1923 0.43 -0.0
2,000,849 - fliC b1923 0.48 +0.8
2,000,935 + fliC b1923 0.54 +1.0
2,000,935 + fliC b1923 0.54 -0.2
2,000,954 + fliC b1923 0.55 +0.4
2,000,981 - fliC b1923 0.57 +0.7
2,001,074 - fliC b1923 0.63 +0.9
2,001,074 - fliC b1923 0.63 +0.7
2,001,083 - fliC b1923 0.63 +0.3
2,001,083 - fliC b1923 0.63 +1.9
2,001,090 - fliC b1923 0.64 +0.7
2,001,090 - fliC b1923 0.64 +1.3
2,001,329 + fliC b1923 0.80 -0.5
2,001,337 - fliC b1923 0.80 +0.5
2,001,337 - fliC b1923 0.80 -0.2
2,001,400 + fliC b1923 0.85 +0.4
2,001,499 - +1.3
2,001,522 - -2.9
2,001,522 - -0.6
2,001,548 + +0.4
2,001,548 + -1.6
2,001,706 + -1.2
2,001,706 + -0.2
2,001,842 - +0.3
2,001,923 + -1.1
2,001,964 - -0.3
2,002,119 + fliD b1924 0.16 -3.1
2,002,119 + fliD b1924 0.16 -0.7
2,002,190 - fliD b1924 0.21 +0.8
2,002,353 + fliD b1924 0.32 -0.8
2,002,456 + fliD b1924 0.40 -1.8
2,002,456 + fliD b1924 0.40 -0.1
2,002,547 - fliD b1924 0.46 +0.7

Or see this region's nucleotide sequence