Experiment: carbon source D-Glucose 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt valV and valW are separated by 4 nucleotides valW and ydhR are separated by 107 nucleotides ydhR and ydhS are separated by 125 nucleotides ydhS and ydhT are separated by 11 nucleotides ydhT and ydhU are separated by 3 nucleotides
b1665: valV - tRNA-Val (NCBI), at 1,744,459 to 1,744,535
valV
b1666: valW - tRNA-Val (NCBI), at 1,744,540 to 1,744,616
valW
b1667: ydhR - hypothetical protein (NCBI), at 1,744,724 to 1,745,029
ydhR
b1668: ydhS - conserved protein with FAD/NAD(P)-binding domain (NCBI), at 1,745,155 to 1,746,759
ydhS
b1669: ydhT - hypothetical protein (NCBI), at 1,746,771 to 1,747,583
ydhT
b1670: ydhU - predicted cytochrome (NCBI), at 1,747,587 to 1,748,372
ydhU
Position (kb)
1745
1746
1747 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1744.176 kb on - strand at 1744.194 kb on - strand at 1744.570 kb on + strand, within valW at 1744.671 kb on - strand at 1744.797 kb on - strand, within ydhR at 1744.953 kb on + strand, within ydhR at 1745.178 kb on - strand at 1745.178 kb on - strand at 1745.272 kb on + strand at 1745.288 kb on + strand at 1745.349 kb on + strand, within ydhS at 1745.351 kb on + strand, within ydhS at 1745.351 kb on + strand, within ydhS at 1745.600 kb on - strand, within ydhS at 1745.646 kb on + strand, within ydhS at 1745.649 kb on - strand, within ydhS at 1745.735 kb on - strand, within ydhS at 1745.752 kb on + strand, within ydhS at 1745.752 kb on + strand, within ydhS at 1745.934 kb on + strand, within ydhS at 1746.064 kb on + strand, within ydhS at 1746.072 kb on - strand, within ydhS at 1746.072 kb on - strand, within ydhS at 1746.195 kb on + strand, within ydhS at 1746.347 kb on + strand, within ydhS at 1746.526 kb on - strand, within ydhS at 1746.854 kb on - strand, within ydhT at 1746.877 kb on + strand, within ydhT at 1746.969 kb on + strand, within ydhT
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.5mM remove 1,744,176 - -0.3 1,744,194 - -0.1 1,744,570 + valW b1666 0.39 -0.3 1,744,671 - -0.3 1,744,797 - ydhR b1667 0.24 +1.4 1,744,953 + ydhR b1667 0.75 +0.3 1,745,178 - +0.8 1,745,178 - -0.3 1,745,272 + -0.7 1,745,288 + -0.1 1,745,349 + ydhS b1668 0.12 +1.1 1,745,351 + ydhS b1668 0.12 +0.5 1,745,351 + ydhS b1668 0.12 +0.1 1,745,600 - ydhS b1668 0.28 +2.2 1,745,646 + ydhS b1668 0.31 -0.5 1,745,649 - ydhS b1668 0.31 -1.1 1,745,735 - ydhS b1668 0.36 +0.0 1,745,752 + ydhS b1668 0.37 +0.1 1,745,752 + ydhS b1668 0.37 -0.1 1,745,934 + ydhS b1668 0.49 +0.6 1,746,064 + ydhS b1668 0.57 +0.4 1,746,072 - ydhS b1668 0.57 +2.6 1,746,072 - ydhS b1668 0.57 -1.8 1,746,195 + ydhS b1668 0.65 +0.7 1,746,347 + ydhS b1668 0.74 +1.6 1,746,526 - ydhS b1668 0.85 +0.9 1,746,854 - ydhT b1669 0.10 -0.6 1,746,877 + ydhT b1669 0.13 +0.4 1,746,969 + ydhT b1669 0.24 -0.1
Or see this region's nucleotide sequence