Strain Fitness in Escherichia coli BW25113 around b0548

Experiment: carbon source D-Glucose 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybcM and ylcH are separated by 116 nucleotidesylcH and ybcN overlap by 4 nucleotidesybcN and ninE overlap by 1 nucleotidesninE and ybcO overlap by 8 nucleotidesybcO and rusA overlap by 4 nucleotidesrusA and ylcG overlap by 4 nucleotidesylcG and ybcQ are separated by 85 nucleotides b0546: ybcM - DLP12 prophage; predicted DNA-binding transcriptional regulator (NCBI), at 570,677 to 571,474 ybcM b4588: ylcH - hypothetical protein, DLP12 prophage (RefSeq), at 571,591 to 571,692 ylcH b0547: ybcN - DLP12 prophage; predicted protein (NCBI), at 571,689 to 572,144 ybcN b0548: ninE - DLP12 prophage; conserved protein (NCBI), at 572,144 to 572,314 ninE b0549: ybcO - DLP12 prophage; predicted protein (NCBI), at 572,307 to 572,597 ybcO b0550: rusA - DLP12 prophage; endonuclease RUS (NCBI), at 572,594 to 572,956 rusA b4509: ylcG - DLP12 prophage; predicted protein (NCBI), at 572,953 to 573,093 ylcG b0551: ybcQ - DLP12 prophage; predicted antitermination protein (NCBI), at 573,179 to 573,562 ybcQ Position (kb) 572 573Strain fitness (log2 ratio) -2 -1 0 1 2at 571.170 kb on - strand, within ybcMat 571.186 kb on + strand, within ybcMat 571.207 kb on + strand, within ybcMat 571.207 kb on + strand, within ybcMat 571.215 kb on - strand, within ybcMat 571.226 kb on + strand, within ybcMat 571.283 kb on + strand, within ybcMat 571.301 kb on + strand, within ybcMat 571.301 kb on + strand, within ybcMat 571.306 kb on + strand, within ybcMat 571.340 kb on + strand, within ybcMat 571.340 kb on + strand, within ybcMat 571.340 kb on + strand, within ybcMat 571.340 kb on + strand, within ybcMat 571.341 kb on - strand, within ybcMat 571.345 kb on - strand, within ybcMat 571.358 kb on - strand, within ybcMat 571.418 kb on + strandat 571.427 kb on + strandat 571.477 kb on - strandat 571.486 kb on - strandat 571.546 kb on - strandat 571.560 kb on - strandat 571.575 kb on - strandat 571.575 kb on - strandat 571.585 kb on - strandat 571.596 kb on + strandat 571.596 kb on - strandat 571.596 kb on - strandat 571.602 kb on - strand, within ylcHat 571.618 kb on + strand, within ylcHat 571.623 kb on - strand, within ylcHat 571.629 kb on + strand, within ylcHat 571.638 kb on - strand, within ylcHat 571.671 kb on + strand, within ylcHat 571.681 kb on + strand, within ylcHat 571.681 kb on + strand, within ylcHat 571.682 kb on - strandat 571.682 kb on - strandat 571.682 kb on - strandat 571.682 kb on - strandat 571.682 kb on - strandat 571.682 kb on - strandat 571.682 kb on - strandat 571.687 kb on + strandat 571.694 kb on - strandat 571.695 kb on - strandat 571.752 kb on + strand, within ybcNat 571.752 kb on + strand, within ybcNat 571.909 kb on + strand, within ybcNat 571.931 kb on + strand, within ybcNat 571.937 kb on - strand, within ybcNat 571.939 kb on - strand, within ybcNat 571.939 kb on - strand, within ybcNat 572.055 kb on + strand, within ybcNat 572.068 kb on - strand, within ybcNat 572.179 kb on + strand, within ninEat 572.179 kb on + strand, within ninEat 572.179 kb on + strand, within ninEat 572.195 kb on - strand, within ninEat 572.214 kb on - strand, within ninEat 572.343 kb on - strand, within ybcOat 572.557 kb on - strand, within ybcOat 572.617 kb on + strandat 572.625 kb on - strandat 572.679 kb on - strand, within rusAat 572.679 kb on - strand, within rusAat 572.697 kb on - strand, within rusAat 572.697 kb on - strand, within rusAat 572.780 kb on + strand, within rusAat 572.899 kb on - strand, within rusAat 572.981 kb on - strand, within ylcGat 573.031 kb on + strand, within ylcGat 573.031 kb on + strand, within ylcGat 573.078 kb on - strand, within ylcGat 573.080 kb on + strandat 573.088 kb on - strandat 573.094 kb on + strandat 573.141 kb on + strandat 573.164 kb on + strandat 573.164 kb on + strandat 573.183 kb on + strandat 573.186 kb on + strandat 573.224 kb on + strand, within ybcQat 573.224 kb on + strand, within ybcQat 573.231 kb on + strand, within ybcQat 573.231 kb on + strand, within ybcQat 573.239 kb on + strand, within ybcQat 573.242 kb on + strand, within ybcQat 573.247 kb on - strand, within ybcQat 573.267 kb on + strand, within ybcQat 573.267 kb on + strand, within ybcQat 573.310 kb on + strand, within ybcQat 573.310 kb on + strand, within ybcQ

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 0.5mM
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571,170 - ybcM b0546 0.62 -0.4
571,186 + ybcM b0546 0.64 +1.2
571,207 + ybcM b0546 0.66 +0.7
571,207 + ybcM b0546 0.66 -1.8
571,215 - ybcM b0546 0.67 +1.9
571,226 + ybcM b0546 0.69 +0.1
571,283 + ybcM b0546 0.76 -1.8
571,301 + ybcM b0546 0.78 -1.1
571,301 + ybcM b0546 0.78 -0.2
571,306 + ybcM b0546 0.79 +0.8
571,340 + ybcM b0546 0.83 +0.9
571,340 + ybcM b0546 0.83 +1.9
571,340 + ybcM b0546 0.83 -2.0
571,340 + ybcM b0546 0.83 -0.6
571,341 - ybcM b0546 0.83 +1.9
571,345 - ybcM b0546 0.84 -1.2
571,358 - ybcM b0546 0.85 +1.9
571,418 + +0.7
571,427 + +0.6
571,477 - -1.4
571,486 - +1.9
571,546 - -0.9
571,560 - -0.6
571,575 - +0.2
571,575 - -2.4
571,585 - +0.3
571,596 + -2.4
571,596 - -0.4
571,596 - -0.1
571,602 - ylcH b4588 0.11 +0.6
571,618 + ylcH b4588 0.26 -1.8
571,623 - ylcH b4588 0.31 +0.4
571,629 + ylcH b4588 0.37 +1.0
571,638 - ylcH b4588 0.46 -0.5
571,671 + ylcH b4588 0.78 -1.7
571,681 + ylcH b4588 0.88 +0.7
571,681 + ylcH b4588 0.88 -0.8
571,682 - -0.6
571,682 - +0.1
571,682 - -0.4
571,682 - +0.1
571,682 - +0.0
571,682 - +0.5
571,682 - +0.2
571,687 + -0.8
571,694 - +0.4
571,695 - +0.9
571,752 + ybcN b0547 0.14 +1.1
571,752 + ybcN b0547 0.14 -0.6
571,909 + ybcN b0547 0.48 -0.1
571,931 + ybcN b0547 0.53 +0.7
571,937 - ybcN b0547 0.54 +0.2
571,939 - ybcN b0547 0.55 +0.5
571,939 - ybcN b0547 0.55 -0.7
572,055 + ybcN b0547 0.80 -1.1
572,068 - ybcN b0547 0.83 -0.8
572,179 + ninE b0548 0.20 +1.4
572,179 + ninE b0548 0.20 +0.9
572,179 + ninE b0548 0.20 -0.3
572,195 - ninE b0548 0.30 +0.3
572,214 - ninE b0548 0.41 -0.0
572,343 - ybcO b0549 0.12 +1.0
572,557 - ybcO b0549 0.86 +0.1
572,617 + -0.0
572,625 - +0.7
572,679 - rusA b0550 0.23 -1.0
572,679 - rusA b0550 0.23 -0.2
572,697 - rusA b0550 0.28 +1.7
572,697 - rusA b0550 0.28 -2.5
572,780 + rusA b0550 0.51 -1.7
572,899 - rusA b0550 0.84 -1.2
572,981 - ylcG b4509 0.20 +0.5
573,031 + ylcG b4509 0.55 -0.1
573,031 + ylcG b4509 0.55 -0.3
573,078 - ylcG b4509 0.89 +1.9
573,080 + -0.4
573,088 - +1.4
573,094 + +0.8
573,141 + +0.4
573,164 + -0.1
573,164 + -0.4
573,183 + +0.2
573,186 + +0.2
573,224 + ybcQ b0551 0.12 -2.6
573,224 + ybcQ b0551 0.12 +1.4
573,231 + ybcQ b0551 0.14 -0.0
573,231 + ybcQ b0551 0.14 -0.3
573,239 + ybcQ b0551 0.16 +0.6
573,242 + ybcQ b0551 0.16 -0.5
573,247 - ybcQ b0551 0.18 +1.9
573,267 + ybcQ b0551 0.23 -0.5
573,267 + ybcQ b0551 0.23 -1.1
573,310 + ybcQ b0551 0.34 +1.3
573,310 + ybcQ b0551 0.34 -0.4

Or see this region's nucleotide sequence