Experiment: carbon source D-Glucose 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt selD and ydjA are separated by 116 nucleotides ydjA and sppA are separated by 160 nucleotides sppA and ansA are separated by 166 nucleotides
b1764: selD - selenophosphate synthetase (NCBI), at 1,844,989 to 1,846,032
selD
b1765: ydjA - predicted oxidoreductase (NCBI), at 1,846,149 to 1,846,700
ydjA
b1766: sppA - protease IV (signal peptide peptidase) (NCBI), at 1,846,861 to 1,848,717
sppA
b1767: ansA - cytoplasmic asparaginase I (NCBI), at 1,848,884 to 1,849,900
ansA
Position (kb)
1846
1847
1848
1849 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1845.881 kb on - strand, within selD at 1845.883 kb on - strand, within selD at 1846.260 kb on + strand, within ydjA at 1846.671 kb on - strand at 1846.671 kb on - strand at 1846.826 kb on - strand at 1846.828 kb on + strand at 1846.843 kb on - strand at 1846.849 kb on - strand at 1846.849 kb on - strand at 1846.860 kb on + strand at 1846.860 kb on + strand at 1846.910 kb on - strand at 1847.043 kb on - strand at 1847.186 kb on - strand, within sppA at 1847.251 kb on + strand, within sppA at 1847.251 kb on + strand, within sppA at 1847.481 kb on + strand, within sppA at 1847.481 kb on + strand at 1847.547 kb on - strand, within sppA at 1847.614 kb on - strand, within sppA at 1847.630 kb on - strand, within sppA at 1847.630 kb on - strand, within sppA at 1847.845 kb on + strand, within sppA at 1847.845 kb on + strand, within sppA at 1847.975 kb on + strand, within sppA at 1848.206 kb on + strand, within sppA at 1848.219 kb on - strand, within sppA at 1848.280 kb on + strand, within sppA at 1848.374 kb on + strand, within sppA at 1848.374 kb on + strand, within sppA at 1848.390 kb on + strand, within sppA at 1848.390 kb on + strand, within sppA at 1848.398 kb on - strand, within sppA at 1848.726 kb on - strand at 1848.833 kb on - strand at 1848.833 kb on - strand at 1848.842 kb on + strand at 1848.842 kb on + strand at 1848.850 kb on - strand at 1848.979 kb on - strand at 1848.979 kb on - strand at 1849.035 kb on - strand, within ansA at 1849.123 kb on + strand, within ansA at 1849.152 kb on + strand, within ansA at 1849.152 kb on + strand, within ansA at 1849.160 kb on - strand, within ansA at 1849.160 kb on - strand, within ansA at 1849.173 kb on - strand, within ansA at 1849.184 kb on - strand, within ansA at 1849.260 kb on - strand, within ansA at 1849.474 kb on - strand, within ansA at 1849.474 kb on - strand, within ansA at 1849.603 kb on + strand, within ansA
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Glucose 1mM remove 1,845,881 - selD b1764 0.85 +0.8 1,845,883 - selD b1764 0.86 -0.7 1,846,260 + ydjA b1765 0.20 -3.8 1,846,671 - -2.5 1,846,671 - +0.1 1,846,826 - -1.8 1,846,828 + +1.5 1,846,843 - -1.0 1,846,849 - -1.3 1,846,849 - -3.1 1,846,860 + -0.2 1,846,860 + +0.0 1,846,910 - -0.0 1,847,043 - -1.6 1,847,186 - sppA b1766 0.18 +0.7 1,847,251 + sppA b1766 0.21 +0.8 1,847,251 + sppA b1766 0.21 +0.2 1,847,481 + sppA b1766 0.33 +0.5 1,847,481 + +1.7 1,847,547 - sppA b1766 0.37 +1.2 1,847,614 - sppA b1766 0.41 +1.3 1,847,630 - sppA b1766 0.41 +0.0 1,847,630 - sppA b1766 0.41 +0.7 1,847,845 + sppA b1766 0.53 -4.4 1,847,845 + sppA b1766 0.53 +0.5 1,847,975 + sppA b1766 0.60 -0.6 1,848,206 + sppA b1766 0.72 +0.4 1,848,219 - sppA b1766 0.73 -0.1 1,848,280 + sppA b1766 0.76 +0.6 1,848,374 + sppA b1766 0.81 -1.3 1,848,374 + sppA b1766 0.81 -1.9 1,848,390 + sppA b1766 0.82 +0.4 1,848,390 + sppA b1766 0.82 -1.9 1,848,398 - sppA b1766 0.83 -2.1 1,848,726 - +0.7 1,848,833 - +0.8 1,848,833 - -3.4 1,848,842 + +0.7 1,848,842 + -0.0 1,848,850 - -0.5 1,848,979 - -0.5 1,848,979 - +0.4 1,849,035 - ansA b1767 0.15 +0.1 1,849,123 + ansA b1767 0.24 +0.5 1,849,152 + ansA b1767 0.26 -0.1 1,849,152 + ansA b1767 0.26 +0.4 1,849,160 - ansA b1767 0.27 +1.3 1,849,160 - ansA b1767 0.27 -3.7 1,849,173 - ansA b1767 0.28 -0.0 1,849,184 - ansA b1767 0.29 +0.1 1,849,260 - ansA b1767 0.37 -0.2 1,849,474 - ansA b1767 0.58 +0.9 1,849,474 - ansA b1767 0.58 -1.2 1,849,603 + ansA b1767 0.71 -0.6
Or see this region's nucleotide sequence