Experiment: carbon source D-Mannose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ytfN and ytfP are separated by 2 nucleotides ytfP and chpS are separated by 211 nucleotides chpS and chpB overlap by 7 nucleotides chpB and ppa are separated by 79 nucleotides ppa and ytfQ are separated by 309 nucleotides
b4221: ytfN - hypothetical protein (NCBI), at 4,442,135 to 4,445,914
ytfN
b4222: ytfP - hypothetical protein (NCBI), at 4,445,917 to 4,446,258
ytfP
b4224: chpS - suppressor of inhibitory function of ChpB, PemI-like, autoregulated (VIMSS), at 4,446,470 to 4,446,721
chpS
b4225: chpB - toxin of the ChpB-ChpS toxin-antitoxin system (NCBI), at 4,446,715 to 4,447,065
chpB
b4226: ppa - inorganic pyrophosphatase (NCBI), at 4,447,145 to 4,447,675
ppa
b4227: ytfQ - predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 4,447,985 to 4,448,941
ytfQ
Position (kb)
4446
4447
4448 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4445.736 kb on + strand at 4445.737 kb on + strand at 4445.737 kb on + strand at 4445.770 kb on - strand at 4445.794 kb on + strand at 4445.806 kb on + strand at 4445.806 kb on + strand at 4445.806 kb on + strand at 4445.836 kb on + strand at 4445.836 kb on + strand at 4445.859 kb on - strand at 4445.866 kb on + strand at 4445.866 kb on + strand at 4445.962 kb on + strand, within ytfP at 4445.962 kb on + strand, within ytfP at 4446.046 kb on - strand, within ytfP at 4446.131 kb on + strand, within ytfP at 4446.166 kb on + strand, within ytfP at 4446.166 kb on + strand, within ytfP at 4446.166 kb on + strand, within ytfP at 4446.176 kb on + strand, within ytfP at 4446.176 kb on - strand, within ytfP at 4446.176 kb on - strand, within ytfP at 4446.176 kb on - strand, within ytfP at 4446.184 kb on - strand, within ytfP at 4446.184 kb on - strand, within ytfP at 4446.240 kb on + strand at 4446.259 kb on + strand at 4446.260 kb on + strand at 4446.272 kb on - strand at 4446.273 kb on - strand at 4446.273 kb on - strand at 4446.375 kb on - strand at 4446.454 kb on + strand at 4446.454 kb on + strand at 4446.496 kb on + strand, within chpS at 4446.501 kb on + strand, within chpS at 4446.501 kb on + strand, within chpS at 4446.509 kb on - strand, within chpS at 4446.509 kb on - strand, within chpS at 4446.514 kb on - strand, within chpS at 4446.559 kb on + strand, within chpS at 4446.569 kb on - strand, within chpS at 4446.569 kb on - strand, within chpS at 4446.646 kb on - strand, within chpS at 4446.652 kb on - strand, within chpS at 4446.746 kb on + strand at 4446.777 kb on + strand, within chpB at 4446.787 kb on + strand, within chpB at 4446.787 kb on + strand, within chpB at 4446.787 kb on - strand, within chpB at 4446.787 kb on - strand, within chpB at 4446.794 kb on + strand, within chpB at 4446.910 kb on + strand, within chpB at 4446.916 kb on - strand, within chpB at 4446.945 kb on + strand, within chpB at 4446.949 kb on - strand, within chpB at 4446.960 kb on + strand, within chpB at 4447.006 kb on - strand, within chpB at 4447.006 kb on - strand, within chpB at 4447.057 kb on + strand at 4447.060 kb on + strand at 4447.060 kb on + strand at 4447.779 kb on + strand at 4447.787 kb on + strand at 4447.792 kb on + strand at 4447.795 kb on - strand at 4447.795 kb on - strand at 4447.797 kb on + strand at 4447.970 kb on + strand at 4447.978 kb on - strand at 4447.983 kb on - strand at 4447.983 kb on - strand at 4448.010 kb on + strand at 4448.039 kb on + strand at 4448.039 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Mannose 5mM remove 4,445,736 + -3.0 4,445,737 + -0.2 4,445,737 + -0.6 4,445,770 - -2.2 4,445,794 + +0.9 4,445,806 + +0.3 4,445,806 + -1.8 4,445,806 + -0.3 4,445,836 + -0.9 4,445,836 + +0.4 4,445,859 - -2.8 4,445,866 + +1.6 4,445,866 + -2.5 4,445,962 + ytfP b4222 0.13 -0.0 4,445,962 + ytfP b4222 0.13 +1.9 4,446,046 - ytfP b4222 0.38 -2.8 4,446,131 + ytfP b4222 0.63 -1.1 4,446,166 + ytfP b4222 0.73 +1.1 4,446,166 + ytfP b4222 0.73 -1.1 4,446,166 + ytfP b4222 0.73 +1.6 4,446,176 + ytfP b4222 0.76 +1.5 4,446,176 - ytfP b4222 0.76 +1.4 4,446,176 - ytfP b4222 0.76 -1.7 4,446,176 - ytfP b4222 0.76 -1.6 4,446,184 - ytfP b4222 0.78 +1.0 4,446,184 - ytfP b4222 0.78 -1.8 4,446,240 + -2.9 4,446,259 + +1.0 4,446,260 + +1.2 4,446,272 - +0.7 4,446,273 - +0.2 4,446,273 - -2.0 4,446,375 - +0.8 4,446,454 + +3.7 4,446,454 + +1.4 4,446,496 + chpS b4224 0.10 +1.4 4,446,501 + chpS b4224 0.12 +0.1 4,446,501 + chpS b4224 0.12 +1.2 4,446,509 - chpS b4224 0.15 +1.9 4,446,509 - chpS b4224 0.15 -1.2 4,446,514 - chpS b4224 0.17 -2.5 4,446,559 + chpS b4224 0.35 +1.4 4,446,569 - chpS b4224 0.39 +2.9 4,446,569 - chpS b4224 0.39 +1.5 4,446,646 - chpS b4224 0.70 +0.9 4,446,652 - chpS b4224 0.72 +0.0 4,446,746 + -2.5 4,446,777 + chpB b4225 0.18 -4.6 4,446,787 + chpB b4225 0.21 +0.6 4,446,787 + chpB b4225 0.21 +0.2 4,446,787 - chpB b4225 0.21 +1.2 4,446,787 - chpB b4225 0.21 +0.6 4,446,794 + chpB b4225 0.23 +3.6 4,446,910 + chpB b4225 0.56 -3.7 4,446,916 - chpB b4225 0.57 +1.3 4,446,945 + chpB b4225 0.66 +2.3 4,446,949 - chpB b4225 0.67 -0.9 4,446,960 + chpB b4225 0.70 +2.1 4,447,006 - chpB b4225 0.83 -2.9 4,447,006 - chpB b4225 0.83 -1.8 4,447,057 + -1.1 4,447,060 + +1.7 4,447,060 + -0.6 4,447,779 + -0.1 4,447,787 + -3.5 4,447,792 + -0.7 4,447,795 - +1.0 4,447,795 - +0.2 4,447,797 + -1.5 4,447,970 + +0.5 4,447,978 - +0.9 4,447,983 - -2.0 4,447,983 - -2.3 4,448,010 + +1.9 4,448,039 + -2.0 4,448,039 - -0.1
Or see this region's nucleotide sequence