Strain Fitness in Escherichia coli BW25113 around b3020

Experiment: carbon source D-Mannose 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntparC and ygiS are separated by 137 nucleotidesygiS and ygiT are separated by 132 nucleotidesygiT and b3022 are separated by 1 nucleotides b3019: parC - DNA topoisomerase IV subunit A (NCBI), at 3,161,737 to 3,163,995 parC b3020: ygiS - predicted transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 3,164,133 to 3,165,740 ygiS b3021: ygiT - predicted DNA-binding transcriptional regulator (NCBI), at 3,165,873 to 3,166,268 ygiT b3022: b3022 - predicted cyanide hydratase (NCBI), at 3,166,270 to 3,166,566 b3022 Position (kb) 3164 3165 3166Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 3163.990 kb on - strandat 3164.001 kb on - strandat 3164.032 kb on - strandat 3164.032 kb on - strandat 3164.032 kb on - strandat 3164.042 kb on - strandat 3164.042 kb on - strandat 3164.096 kb on - strandat 3164.130 kb on + strandat 3164.148 kb on + strandat 3164.148 kb on + strandat 3164.151 kb on - strandat 3164.160 kb on + strandat 3164.217 kb on - strandat 3164.255 kb on - strandat 3164.334 kb on + strand, within ygiSat 3164.337 kb on - strand, within ygiSat 3164.344 kb on + strand, within ygiSat 3164.346 kb on + strand, within ygiSat 3164.346 kb on + strand, within ygiSat 3164.352 kb on - strand, within ygiSat 3164.367 kb on - strand, within ygiSat 3164.367 kb on - strand, within ygiSat 3164.445 kb on - strand, within ygiSat 3164.511 kb on - strand, within ygiSat 3164.513 kb on + strand, within ygiSat 3164.520 kb on - strand, within ygiSat 3164.520 kb on - strand, within ygiSat 3164.608 kb on - strand, within ygiSat 3164.622 kb on - strand, within ygiSat 3164.622 kb on - strand, within ygiSat 3164.633 kb on - strand, within ygiSat 3164.791 kb on - strand, within ygiSat 3164.827 kb on - strand, within ygiSat 3164.827 kb on - strand, within ygiSat 3164.942 kb on + strand, within ygiSat 3164.953 kb on - strand, within ygiSat 3164.953 kb on - strand, within ygiSat 3164.984 kb on - strand, within ygiSat 3164.984 kb on - strand, within ygiSat 3164.984 kb on - strand, within ygiSat 3165.101 kb on + strand, within ygiSat 3165.112 kb on + strand, within ygiSat 3165.112 kb on + strand, within ygiSat 3165.114 kb on + strand, within ygiSat 3165.122 kb on - strand, within ygiSat 3165.147 kb on - strand, within ygiSat 3165.147 kb on - strand, within ygiSat 3165.196 kb on - strand, within ygiSat 3165.256 kb on - strand, within ygiSat 3165.256 kb on - strand, within ygiSat 3165.304 kb on + strand, within ygiSat 3165.442 kb on + strand, within ygiSat 3165.443 kb on - strand, within ygiSat 3165.477 kb on + strand, within ygiSat 3165.516 kb on - strand, within ygiSat 3165.530 kb on - strand, within ygiSat 3165.530 kb on - strand, within ygiSat 3165.544 kb on + strand, within ygiSat 3165.545 kb on - strand, within ygiSat 3165.545 kb on - strand, within ygiSat 3165.552 kb on - strand, within ygiSat 3165.574 kb on + strand, within ygiSat 3165.583 kb on - strandat 3165.583 kb on - strandat 3165.616 kb on - strandat 3165.667 kb on - strandat 3165.682 kb on + strandat 3165.682 kb on + strandat 3165.687 kb on - strandat 3165.692 kb on + strandat 3165.692 kb on - strandat 3165.692 kb on - strandat 3165.700 kb on + strandat 3165.736 kb on + strandat 3165.745 kb on - strandat 3165.748 kb on - strandat 3165.770 kb on + strandat 3165.775 kb on + strandat 3165.784 kb on - strandat 3165.798 kb on + strandat 3165.798 kb on + strandat 3165.822 kb on + strandat 3165.822 kb on + strandat 3165.895 kb on + strandat 3165.903 kb on + strandat 3165.914 kb on - strand, within ygiTat 3165.946 kb on - strand, within ygiTat 3165.950 kb on - strand, within ygiTat 3165.950 kb on - strand, within ygiTat 3165.985 kb on - strand, within ygiTat 3166.018 kb on - strand, within ygiTat 3166.093 kb on + strand, within ygiTat 3166.285 kb on - strandat 3166.285 kb on - strandat 3166.286 kb on - strandat 3166.290 kb on - strandat 3166.290 kb on - strandat 3166.299 kb on - strandat 3166.308 kb on - strand, within b3022at 3166.308 kb on - strand, within b3022at 3166.325 kb on - strand, within b3022at 3166.330 kb on - strand, within b3022at 3166.330 kb on - strand, within b3022at 3166.335 kb on - strand, within b3022at 3166.337 kb on + strand, within b3022at 3166.345 kb on - strand, within b3022at 3166.345 kb on - strand, within b3022at 3166.346 kb on - strand, within b3022at 3166.357 kb on - strand, within b3022at 3166.370 kb on + strand, within b3022at 3166.376 kb on - strand, within b3022at 3166.377 kb on + strand, within b3022at 3166.382 kb on + strand, within b3022at 3166.382 kb on + strand, within b3022at 3166.388 kb on - strand, within b3022at 3166.388 kb on - strand, within b3022at 3166.390 kb on - strand, within b3022at 3166.420 kb on - strand, within b3022at 3166.441 kb on - strand, within b3022at 3166.480 kb on - strand, within b3022at 3166.480 kb on - strand, within b3022at 3166.523 kb on + strand, within b3022at 3166.539 kb on + strandat 3166.551 kb on - strandat 3166.551 kb on - strandat 3166.551 kb on - strandat 3166.576 kb on - strandat 3166.576 kb on - strandat 3166.696 kb on - strandat 3166.703 kb on + strandat 3166.703 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 5mM
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3,163,990 - -4.0
3,164,001 - -3.4
3,164,032 - -1.9
3,164,032 - +1.0
3,164,032 - -0.2
3,164,042 - +2.5
3,164,042 - -0.2
3,164,096 - -3.7
3,164,130 + -3.6
3,164,148 + -2.4
3,164,148 + +1.0
3,164,151 - +1.1
3,164,160 + -3.2
3,164,217 - +0.3
3,164,255 - +1.8
3,164,334 + ygiS b3020 0.12 -0.9
3,164,337 - ygiS b3020 0.13 -0.2
3,164,344 + ygiS b3020 0.13 +1.0
3,164,346 + ygiS b3020 0.13 +0.5
3,164,346 + ygiS b3020 0.13 -1.3
3,164,352 - ygiS b3020 0.14 +1.9
3,164,367 - ygiS b3020 0.15 -0.2
3,164,367 - ygiS b3020 0.15 +0.4
3,164,445 - ygiS b3020 0.19 -0.8
3,164,511 - ygiS b3020 0.24 -2.4
3,164,513 + ygiS b3020 0.24 +0.5
3,164,520 - ygiS b3020 0.24 -1.9
3,164,520 - ygiS b3020 0.24 +2.1
3,164,608 - ygiS b3020 0.30 -3.1
3,164,622 - ygiS b3020 0.30 +1.0
3,164,622 - ygiS b3020 0.30 +0.2
3,164,633 - ygiS b3020 0.31 +0.4
3,164,791 - ygiS b3020 0.41 +1.1
3,164,827 - ygiS b3020 0.43 +0.9
3,164,827 - ygiS b3020 0.43 -2.1
3,164,942 + ygiS b3020 0.50 +2.0
3,164,953 - ygiS b3020 0.51 +0.1
3,164,953 - ygiS b3020 0.51 +2.2
3,164,984 - ygiS b3020 0.53 +0.7
3,164,984 - ygiS b3020 0.53 -3.1
3,164,984 - ygiS b3020 0.53 -0.5
3,165,101 + ygiS b3020 0.60 -0.8
3,165,112 + ygiS b3020 0.61 +1.5
3,165,112 + ygiS b3020 0.61 -2.0
3,165,114 + ygiS b3020 0.61 +1.1
3,165,122 - ygiS b3020 0.62 +2.1
3,165,147 - ygiS b3020 0.63 -1.9
3,165,147 - ygiS b3020 0.63 -2.7
3,165,196 - ygiS b3020 0.66 +0.7
3,165,256 - ygiS b3020 0.70 -2.3
3,165,256 - ygiS b3020 0.70 +1.2
3,165,304 + ygiS b3020 0.73 +0.9
3,165,442 + ygiS b3020 0.81 +0.3
3,165,443 - ygiS b3020 0.81 +0.8
3,165,477 + ygiS b3020 0.84 -3.0
3,165,516 - ygiS b3020 0.86 +1.4
3,165,530 - ygiS b3020 0.87 -3.3
3,165,530 - ygiS b3020 0.87 -0.5
3,165,544 + ygiS b3020 0.88 -0.6
3,165,545 - ygiS b3020 0.88 +1.5
3,165,545 - ygiS b3020 0.88 +0.8
3,165,552 - ygiS b3020 0.88 +0.9
3,165,574 + ygiS b3020 0.90 +2.5
3,165,583 - -2.4
3,165,583 - +0.7
3,165,616 - -0.1
3,165,667 - -2.2
3,165,682 + -3.6
3,165,682 + -2.0
3,165,687 - -2.3
3,165,692 + +1.4
3,165,692 - -1.8
3,165,692 - -2.1
3,165,700 + +1.5
3,165,736 + +0.1
3,165,745 - -0.9
3,165,748 - -1.8
3,165,770 + -3.4
3,165,775 + -1.4
3,165,784 - -1.2
3,165,798 + -2.2
3,165,798 + -2.5
3,165,822 + -0.7
3,165,822 + -2.3
3,165,895 + +2.0
3,165,903 + +3.2
3,165,914 - ygiT b3021 0.10 +0.6
3,165,946 - ygiT b3021 0.18 +1.2
3,165,950 - ygiT b3021 0.19 -0.5
3,165,950 - ygiT b3021 0.19 +0.6
3,165,985 - ygiT b3021 0.28 +2.4
3,166,018 - ygiT b3021 0.37 -0.6
3,166,093 + ygiT b3021 0.56 +2.2
3,166,285 - -0.1
3,166,285 - +0.0
3,166,286 - +1.4
3,166,290 - +1.8
3,166,290 - -0.6
3,166,299 - +1.1
3,166,308 - b3022 b3022 0.13 +0.5
3,166,308 - b3022 b3022 0.13 -0.8
3,166,325 - b3022 b3022 0.19 +0.7
3,166,330 - b3022 b3022 0.20 -3.3
3,166,330 - b3022 b3022 0.20 +0.4
3,166,335 - b3022 b3022 0.22 -4.0
3,166,337 + b3022 b3022 0.23 +0.9
3,166,345 - b3022 b3022 0.25 +1.1
3,166,345 - b3022 b3022 0.25 +0.3
3,166,346 - b3022 b3022 0.26 +0.6
3,166,357 - b3022 b3022 0.29 +1.4
3,166,370 + b3022 b3022 0.34 +1.5
3,166,376 - b3022 b3022 0.36 +1.7
3,166,377 + b3022 b3022 0.36 -1.4
3,166,382 + b3022 b3022 0.38 -0.1
3,166,382 + b3022 b3022 0.38 +1.6
3,166,388 - b3022 b3022 0.40 +0.0
3,166,388 - b3022 b3022 0.40 -2.3
3,166,390 - b3022 b3022 0.40 +0.0
3,166,420 - b3022 b3022 0.51 -1.2
3,166,441 - b3022 b3022 0.58 -1.6
3,166,480 - b3022 b3022 0.71 -1.1
3,166,480 - b3022 b3022 0.71 -1.1
3,166,523 + b3022 b3022 0.85 +0.2
3,166,539 + +1.6
3,166,551 - -0.1
3,166,551 - +0.7
3,166,551 - +0.4
3,166,576 - -0.5
3,166,576 - -1.9
3,166,696 - +0.6
3,166,703 + +0.4
3,166,703 + +1.4

Or see this region's nucleotide sequence