Experiment: carbon source D-Mannose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt citG and citX overlap by 26 nucleotides citX and citF are separated by 3 nucleotides citF and citE are separated by 10 nucleotides citE and citD overlap by 4 nucleotides
b0613: citG - triphosphoribosyl-dephospho-CoA transferase (NCBI), at 645,854 to 646,732
citG
b0614: citX - 2'-(5'-triphosphoribosyl)-3'-dephospho-CoA:apo- citrate lyase (NCBI), at 646,707 to 647,258
citX
b0615: citF - citrate lyase, citrate-ACP transferase (alpha) subunit (NCBI), at 647,262 to 648,794
citF
b0616: citE - citrate lyase beta chain (acyl lyase subunit) (VIMSS), at 648,805 to 649,713
citE
b0617: citD - citrate lyase, acyl carrier (gamma) subunit (NCBI), at 649,710 to 650,006
citD
Position (kb) 647
648
649 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 646.308 kb on - strand, within citG at 646.308 kb on - strand, within citG at 646.530 kb on - strand, within citG at 646.595 kb on + strand, within citG at 646.595 kb on + strand, within citG at 646.656 kb on + strand at 646.666 kb on + strand at 646.666 kb on + strand at 646.675 kb on - strand at 646.675 kb on - strand at 647.268 kb on - strand at 647.354 kb on + strand at 647.383 kb on - strand at 647.428 kb on + strand, within citF at 647.428 kb on + strand, within citF at 647.522 kb on + strand, within citF at 647.601 kb on + strand, within citF at 648.197 kb on + strand, within citF at 648.197 kb on + strand, within citF at 648.551 kb on - strand, within citF at 648.551 kb on - strand, within citF at 648.610 kb on - strand, within citF at 648.645 kb on - strand at 648.676 kb on + strand at 648.676 kb on + strand at 648.810 kb on - strand at 649.122 kb on - strand, within citE at 649.238 kb on - strand, within citE at 649.238 kb on - strand, within citE at 649.327 kb on - strand, within citE at 649.479 kb on + strand, within citE at 649.540 kb on - strand, within citE at 649.569 kb on + strand, within citE at 649.595 kb on - strand, within citE at 649.595 kb on - strand, within citE at 649.607 kb on + strand, within citE at 649.642 kb on + strand at 649.665 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source D-Mannose 5mM remove 646,308 - citG b0613 0.52 -2.8 646,308 - citG b0613 0.52 -2.7 646,530 - citG b0613 0.77 -4.1 646,595 + citG b0613 0.84 +0.5 646,595 + citG b0613 0.84 -0.9 646,656 + +1.1 646,666 + +1.9 646,666 + -2.3 646,675 - +0.0 646,675 - -3.4 647,268 - +0.1 647,354 + +1.0 647,383 - -3.0 647,428 + citF b0615 0.11 -4.0 647,428 + citF b0615 0.11 -0.9 647,522 + citF b0615 0.17 -1.1 647,601 + citF b0615 0.22 +2.2 648,197 + citF b0615 0.61 -3.4 648,197 + citF b0615 0.61 +0.2 648,551 - citF b0615 0.84 -1.8 648,551 - citF b0615 0.84 -2.8 648,610 - citF b0615 0.88 -2.4 648,645 - +0.3 648,676 + -1.8 648,676 + -2.2 648,810 - -0.9 649,122 - citE b0616 0.35 -0.2 649,238 - citE b0616 0.48 -2.2 649,238 - citE b0616 0.48 -2.6 649,327 - citE b0616 0.57 +1.2 649,479 + citE b0616 0.74 +0.9 649,540 - citE b0616 0.81 -3.3 649,569 + citE b0616 0.84 -0.7 649,595 - citE b0616 0.87 +0.1 649,595 - citE b0616 0.87 -0.5 649,607 + citE b0616 0.88 -3.6 649,642 + -0.4 649,665 - -1.2
Or see this region's nucleotide sequence