Strain Fitness in Escherichia coli BW25113 around b4221
Experiment: carbon source D-Mannose 20mM
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | carbon source D-Mannose 20mM |
---|---|---|---|---|---|
remove | |||||
4,441,144 | - | ytfM | b4220 | 0.43 | +0.9 |
4,441,234 | - | ytfM | b4220 | 0.48 | +0.7 |
4,441,234 | - | ytfM | b4220 | 0.48 | +0.0 |
4,441,292 | + | ytfM | b4220 | 0.51 | +0.7 |
4,441,306 | + | ytfM | b4220 | 0.52 | +0.6 |
4,441,382 | + | ytfM | b4220 | 0.56 | +0.1 |
4,441,406 | + | ytfM | b4220 | 0.58 | +0.9 |
4,441,406 | + | ytfM | b4220 | 0.58 | +1.1 |
4,441,416 | + | ytfM | b4220 | 0.58 | +1.2 |
4,441,424 | - | ytfM | b4220 | 0.59 | +1.2 |
4,441,441 | + | ytfM | b4220 | 0.60 | +3.0 |
4,441,441 | + | ytfM | b4220 | 0.60 | +1.9 |
4,441,442 | + | ytfM | b4220 | 0.60 | +1.1 |
4,441,456 | + | ytfM | b4220 | 0.61 | +1.1 |
4,441,458 | + | ytfM | b4220 | 0.61 | +0.7 |
4,441,495 | + | ytfM | b4220 | 0.63 | -0.3 |
4,441,503 | - | ytfM | b4220 | 0.63 | +0.7 |
4,441,521 | - | ytfM | b4220 | 0.64 | -0.5 |
4,441,559 | + | ytfM | b4220 | 0.67 | +1.4 |
4,441,564 | + | ytfM | b4220 | 0.67 | +1.3 |
4,441,564 | + | ytfM | b4220 | 0.67 | +0.3 |
4,441,567 | - | ytfM | b4220 | 0.67 | +0.5 |
4,441,614 | + | ytfM | b4220 | 0.70 | +0.9 |
4,441,614 | + | ytfM | b4220 | 0.70 | -0.0 |
4,441,639 | + | ytfM | b4220 | 0.71 | +1.8 |
4,441,639 | + | ytfM | b4220 | 0.71 | -1.5 |
4,441,653 | + | ytfM | b4220 | 0.72 | +0.6 |
4,441,657 | - | ytfM | b4220 | 0.72 | +0.3 |
4,441,657 | - | ytfM | b4220 | 0.72 | +1.0 |
4,441,657 | - | ytfM | b4220 | 0.72 | +0.1 |
4,441,717 | + | ytfM | b4220 | 0.76 | +1.0 |
4,441,727 | - | ytfM | b4220 | 0.76 | -0.1 |
4,441,828 | + | ytfM | b4220 | 0.82 | +0.8 |
4,441,933 | - | ytfM | b4220 | 0.88 | +0.4 |
4,441,933 | - | ytfM | b4220 | 0.88 | +1.1 |
4,441,969 | - | -0.3 | |||
4,441,969 | - | +0.5 | |||
4,442,033 | - | +1.0 | |||
4,442,108 | + | +2.0 | |||
4,442,183 | + | +0.8 | |||
4,442,201 | - | +2.4 | |||
4,442,225 | + | +0.7 | |||
4,442,262 | + | +0.6 | |||
4,442,262 | + | -0.6 | |||
4,442,264 | - | +0.5 | |||
4,442,303 | + | +0.5 | |||
4,442,303 | + | +1.1 | |||
4,442,312 | - | +1.1 | |||
4,442,400 | + | +0.8 | |||
4,442,448 | - | +0.7 | |||
4,442,448 | - | -1.0 | |||
4,442,469 | + | +0.7 | |||
4,442,469 | + | +0.9 | |||
4,442,512 | - | +1.0 | |||
4,442,515 | + | ytfN | b4221 | 0.10 | +0.3 |
4,442,535 | + | ytfN | b4221 | 0.11 | +1.6 |
4,442,535 | + | ytfN | b4221 | 0.11 | +0.8 |
4,442,542 | - | ytfN | b4221 | 0.11 | +1.0 |
4,442,543 | - | ytfN | b4221 | 0.11 | +1.2 |
4,442,544 | + | ytfN | b4221 | 0.11 | +0.8 |
4,442,544 | + | ytfN | b4221 | 0.11 | +0.6 |
4,442,549 | + | ytfN | b4221 | 0.11 | +1.1 |
4,442,557 | - | ytfN | b4221 | 0.11 | -0.4 |
4,442,557 | - | ytfN | b4221 | 0.11 | +1.1 |
4,442,557 | - | ytfN | b4221 | 0.11 | +1.5 |
4,442,571 | + | ytfN | b4221 | 0.12 | +1.0 |
4,442,571 | + | ytfN | b4221 | 0.12 | +1.5 |
4,442,594 | - | ytfN | b4221 | 0.12 | +0.4 |
4,442,634 | + | ytfN | b4221 | 0.13 | +0.6 |
4,442,634 | + | ytfN | b4221 | 0.13 | +0.8 |
4,442,704 | + | ytfN | b4221 | 0.15 | -0.3 |
4,442,704 | + | ytfN | b4221 | 0.15 | +0.5 |
4,442,818 | - | ytfN | b4221 | 0.18 | +0.1 |
4,442,853 | - | ytfN | b4221 | 0.19 | +0.2 |
4,442,853 | - | ytfN | b4221 | 0.19 | +0.7 |
4,442,855 | + | ytfN | b4221 | 0.19 | +0.9 |
4,442,872 | + | ytfN | b4221 | 0.19 | +2.1 |
4,442,890 | + | ytfN | b4221 | 0.20 | +1.3 |
4,442,890 | + | ytfN | b4221 | 0.20 | +0.9 |
4,442,912 | - | ytfN | b4221 | 0.21 | +0.2 |
4,443,017 | + | ytfN | b4221 | 0.23 | +2.1 |
4,443,017 | + | ytfN | b4221 | 0.23 | +1.3 |
4,443,031 | + | ytfN | b4221 | 0.24 | +0.4 |
4,443,033 | + | ytfN | b4221 | 0.24 | +0.4 |
4,443,041 | - | ytfN | b4221 | 0.24 | +1.2 |
4,443,041 | - | ytfN | b4221 | 0.24 | +0.1 |
4,443,041 | - | ytfN | b4221 | 0.24 | -0.7 |
4,443,041 | - | ytfN | b4221 | 0.24 | +1.8 |
4,443,053 | - | ytfN | b4221 | 0.24 | +0.6 |
4,443,053 | - | ytfN | b4221 | 0.24 | -2.5 |
4,443,106 | + | ytfN | b4221 | 0.26 | +0.5 |
4,443,160 | + | ytfN | b4221 | 0.27 | +0.7 |
4,443,212 | + | ytfN | b4221 | 0.28 | +0.8 |
4,443,212 | + | ytfN | b4221 | 0.28 | -0.0 |
4,443,212 | + | ytfN | b4221 | 0.28 | +1.4 |
4,443,233 | + | ytfN | b4221 | 0.29 | +0.2 |
4,443,244 | + | ytfN | b4221 | 0.29 | +0.8 |
4,443,244 | + | ytfN | b4221 | 0.29 | +0.1 |
4,443,244 | + | ytfN | b4221 | 0.29 | +1.4 |
4,443,250 | + | ytfN | b4221 | 0.29 | +1.3 |
4,443,252 | - | ytfN | b4221 | 0.30 | +1.8 |
4,443,266 | - | ytfN | b4221 | 0.30 | +1.0 |
4,443,309 | - | ytfN | b4221 | 0.31 | +1.0 |
4,443,309 | - | ytfN | b4221 | 0.31 | +0.4 |
4,443,309 | - | ytfN | b4221 | 0.31 | +0.9 |
4,443,314 | + | ytfN | b4221 | 0.31 | +1.2 |
4,443,339 | - | ytfN | b4221 | 0.32 | +0.7 |
4,443,339 | - | ytfN | b4221 | 0.32 | +0.7 |
4,443,447 | - | ytfN | b4221 | 0.35 | +0.6 |
4,443,447 | - | ytfN | b4221 | 0.35 | +0.9 |
4,443,452 | - | ytfN | b4221 | 0.35 | +0.7 |
4,443,565 | - | ytfN | b4221 | 0.38 | +0.6 |
4,443,581 | - | ytfN | b4221 | 0.38 | -2.7 |
4,443,698 | - | ytfN | b4221 | 0.41 | -0.6 |
4,443,719 | - | ytfN | b4221 | 0.42 | +0.6 |
4,443,789 | - | ytfN | b4221 | 0.44 | +1.0 |
4,443,793 | + | ytfN | b4221 | 0.44 | -0.4 |
4,443,793 | + | ytfN | b4221 | 0.44 | -0.4 |
4,443,818 | + | ytfN | b4221 | 0.45 | +1.1 |
4,443,820 | - | ytfN | b4221 | 0.45 | +1.2 |
4,443,840 | + | ytfN | b4221 | 0.45 | +0.8 |
4,444,001 | + | ytfN | b4221 | 0.49 | +1.4 |
4,444,015 | - | ytfN | b4221 | 0.50 | +0.2 |
4,444,015 | - | ytfN | b4221 | 0.50 | +0.9 |
4,444,057 | - | ytfN | b4221 | 0.51 | -0.4 |
4,444,057 | - | ytfN | b4221 | 0.51 | +0.8 |
4,444,058 | - | ytfN | b4221 | 0.51 | +0.3 |
4,444,090 | + | ytfN | b4221 | 0.52 | +0.8 |
4,444,090 | + | ytfN | b4221 | 0.52 | +2.2 |
4,444,121 | + | ytfN | b4221 | 0.53 | +1.5 |
4,444,121 | + | ytfN | b4221 | 0.53 | +1.3 |
4,444,194 | - | ytfN | b4221 | 0.54 | +1.3 |
4,444,194 | - | ytfN | b4221 | 0.54 | -0.2 |
4,444,246 | + | ytfN | b4221 | 0.56 | +1.0 |
4,444,246 | - | ytfN | b4221 | 0.56 | +2.8 |
4,444,263 | + | ytfN | b4221 | 0.56 | +1.6 |
4,444,263 | + | ytfN | b4221 | 0.56 | +2.3 |
4,444,349 | - | ytfN | b4221 | 0.59 | +0.2 |
4,444,349 | - | ytfN | b4221 | 0.59 | +0.3 |
4,444,386 | - | ytfN | b4221 | 0.60 | +0.5 |
4,444,386 | - | ytfN | b4221 | 0.60 | +1.6 |
4,444,391 | - | ytfN | b4221 | 0.60 | -0.5 |
4,444,412 | + | ytfN | b4221 | 0.60 | +1.1 |
4,444,420 | - | ytfN | b4221 | 0.60 | +0.9 |
4,444,444 | + | ytfN | b4221 | 0.61 | +0.1 |
4,444,444 | + | ytfN | b4221 | 0.61 | -1.1 |
4,444,510 | - | ytfN | b4221 | 0.63 | +0.8 |
4,444,570 | - | ytfN | b4221 | 0.64 | +0.4 |
4,444,602 | + | ytfN | b4221 | 0.65 | -0.3 |
4,444,602 | + | ytfN | b4221 | 0.65 | +0.7 |
4,444,602 | + | ytfN | b4221 | 0.65 | +0.5 |
4,444,612 | + | ytfN | b4221 | 0.66 | +0.6 |
4,444,612 | + | ytfN | b4221 | 0.66 | +0.4 |
4,444,626 | - | ytfN | b4221 | 0.66 | +0.5 |
4,444,638 | + | ytfN | b4221 | 0.66 | +1.2 |
4,444,653 | + | ytfN | b4221 | 0.67 | +0.6 |
4,444,653 | + | ytfN | b4221 | 0.67 | +1.0 |
4,444,700 | - | ytfN | b4221 | 0.68 | -0.4 |
4,444,800 | - | ytfN | b4221 | 0.71 | +0.4 |
4,444,895 | + | ytfN | b4221 | 0.73 | +0.5 |
4,444,895 | + | ytfN | b4221 | 0.73 | +1.3 |
4,444,898 | - | ytfN | b4221 | 0.73 | +1.5 |
4,444,898 | - | ytfN | b4221 | 0.73 | +2.1 |
4,444,940 | - | ytfN | b4221 | 0.74 | +0.3 |
4,444,991 | + | ytfN | b4221 | 0.76 | +0.6 |
4,444,991 | + | ytfN | b4221 | 0.76 | +1.7 |
4,445,044 | + | ytfN | b4221 | 0.77 | +1.3 |
4,445,044 | + | ytfN | b4221 | 0.77 | +1.5 |
4,445,063 | - | ytfN | b4221 | 0.77 | -0.0 |
4,445,063 | - | ytfN | b4221 | 0.77 | +2.2 |
4,445,065 | + | ytfN | b4221 | 0.78 | +1.4 |
4,445,077 | - | ytfN | b4221 | 0.78 | -0.1 |
4,445,079 | + | ytfN | b4221 | 0.78 | +1.5 |
4,445,081 | + | ytfN | b4221 | 0.78 | -0.6 |
4,445,118 | + | ytfN | b4221 | 0.79 | +1.6 |
4,445,118 | + | ytfN | b4221 | 0.79 | +0.9 |
4,445,141 | - | ytfN | b4221 | 0.80 | +1.4 |
4,445,154 | + | ytfN | b4221 | 0.80 | +1.5 |
4,445,154 | + | ytfN | b4221 | 0.80 | +0.5 |
4,445,237 | + | ytfN | b4221 | 0.82 | -0.5 |
4,445,252 | - | ytfN | b4221 | 0.82 | +0.5 |
4,445,273 | - | ytfN | b4221 | 0.83 | +1.0 |
4,445,320 | + | ytfN | b4221 | 0.84 | +0.9 |
4,445,535 | - | ytfN | b4221 | 0.90 | +0.7 |
4,445,639 | + | +0.5 | |||
4,445,668 | - | -0.2 | |||
4,445,668 | - | -0.0 | |||
4,445,736 | + | +1.2 | |||
4,445,737 | + | +1.9 | |||
4,445,737 | + | +2.3 | |||
4,445,770 | - | +1.0 | |||
4,445,794 | + | +0.4 | |||
4,445,806 | + | +0.8 | |||
4,445,806 | + | -0.9 | |||
4,445,806 | + | +0.2 | |||
4,445,836 | + | +1.0 | |||
4,445,836 | + | +0.8 | |||
4,445,859 | - | +1.4 | |||
4,445,866 | + | +1.9 | |||
4,445,866 | + | +1.0 | |||
4,445,962 | + | ytfP | b4222 | 0.13 | +0.2 |
4,445,962 | + | ytfP | b4222 | 0.13 | +0.1 |
4,446,046 | - | ytfP | b4222 | 0.38 | -0.4 |
4,446,131 | + | ytfP | b4222 | 0.63 | -1.5 |
4,446,166 | + | ytfP | b4222 | 0.73 | -1.8 |
4,446,166 | + | ytfP | b4222 | 0.73 | +0.5 |
4,446,166 | + | ytfP | b4222 | 0.73 | +0.9 |
4,446,176 | + | ytfP | b4222 | 0.76 | -0.7 |
4,446,176 | - | ytfP | b4222 | 0.76 | -0.6 |
4,446,176 | - | ytfP | b4222 | 0.76 | +1.4 |
4,446,176 | - | ytfP | b4222 | 0.76 | +0.9 |
4,446,184 | - | ytfP | b4222 | 0.78 | +0.2 |
4,446,184 | - | ytfP | b4222 | 0.78 | -0.5 |
4,446,240 | + | +0.5 | |||
4,446,259 | + | -0.9 | |||
4,446,260 | + | +0.6 | |||
4,446,272 | - | -1.3 | |||
4,446,273 | - | -0.8 | |||
4,446,273 | - | -3.1 | |||
4,446,375 | - | -0.7 | |||
4,446,454 | + | +0.1 | |||
4,446,454 | + | -0.1 | |||
4,446,496 | + | chpS | b4224 | 0.10 | +0.4 |
4,446,501 | + | chpS | b4224 | 0.12 | +0.5 |
4,446,501 | + | chpS | b4224 | 0.12 | +0.5 |
4,446,509 | - | chpS | b4224 | 0.15 | +1.3 |
4,446,509 | - | chpS | b4224 | 0.15 | -2.3 |
4,446,514 | - | chpS | b4224 | 0.17 | -2.6 |
4,446,559 | + | chpS | b4224 | 0.35 | +2.2 |
4,446,569 | - | chpS | b4224 | 0.39 | +1.2 |
4,446,569 | - | chpS | b4224 | 0.39 | -0.2 |
4,446,646 | - | chpS | b4224 | 0.70 | +3.8 |
4,446,652 | - | chpS | b4224 | 0.72 | -0.7 |
4,446,746 | + | +0.5 | |||
4,446,777 | + | chpB | b4225 | 0.18 | -0.1 |
4,446,787 | + | chpB | b4225 | 0.21 | -0.2 |
4,446,787 | + | chpB | b4225 | 0.21 | -0.1 |
4,446,787 | - | chpB | b4225 | 0.21 | +1.5 |
4,446,787 | - | chpB | b4225 | 0.21 | -0.2 |
4,446,794 | + | chpB | b4225 | 0.23 | -1.3 |
4,446,910 | + | chpB | b4225 | 0.56 | -1.0 |
Or see this region's nucleotide sequence