Strain Fitness in Escherichia coli BW25113 around b4221

Experiment: carbon source D-Mannose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntytfM and ytfN overlap by 4 nucleotidesytfN and ytfP are separated by 2 nucleotidesytfP and chpS are separated by 211 nucleotideschpS and chpB overlap by 7 nucleotides b4220: ytfM - predicted outer membrane protein and surface antigen (NCBI), at 4,440,405 to 4,442,138 ytfM b4221: ytfN - hypothetical protein (NCBI), at 4,442,135 to 4,445,914 ytfN b4222: ytfP - hypothetical protein (NCBI), at 4,445,917 to 4,446,258 ytfP b4224: chpS - suppressor of inhibitory function of ChpB, PemI-like, autoregulated (VIMSS), at 4,446,470 to 4,446,721 chpS b4225: chpB - toxin of the ChpB-ChpS toxin-antitoxin system (NCBI), at 4,446,715 to 4,447,065 chpB Position (kb) 4442 4443 4444 4445 4446Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 4441.144 kb on - strand, within ytfMat 4441.234 kb on - strand, within ytfMat 4441.234 kb on - strand, within ytfMat 4441.292 kb on + strand, within ytfMat 4441.306 kb on + strand, within ytfMat 4441.382 kb on + strand, within ytfMat 4441.406 kb on + strand, within ytfMat 4441.406 kb on + strand, within ytfMat 4441.416 kb on + strand, within ytfMat 4441.424 kb on - strand, within ytfMat 4441.441 kb on + strand, within ytfMat 4441.441 kb on + strand, within ytfMat 4441.442 kb on + strand, within ytfMat 4441.456 kb on + strand, within ytfMat 4441.458 kb on + strand, within ytfMat 4441.495 kb on + strand, within ytfMat 4441.503 kb on - strand, within ytfMat 4441.521 kb on - strand, within ytfMat 4441.559 kb on + strand, within ytfMat 4441.564 kb on + strand, within ytfMat 4441.564 kb on + strand, within ytfMat 4441.567 kb on - strand, within ytfMat 4441.614 kb on + strand, within ytfMat 4441.614 kb on + strand, within ytfMat 4441.639 kb on + strand, within ytfMat 4441.639 kb on + strand, within ytfMat 4441.653 kb on + strand, within ytfMat 4441.657 kb on - strand, within ytfMat 4441.657 kb on - strand, within ytfMat 4441.657 kb on - strand, within ytfMat 4441.717 kb on + strand, within ytfMat 4441.727 kb on - strand, within ytfMat 4441.828 kb on + strand, within ytfMat 4441.933 kb on - strand, within ytfMat 4441.933 kb on - strand, within ytfMat 4441.969 kb on - strandat 4441.969 kb on - strandat 4442.033 kb on - strandat 4442.108 kb on + strandat 4442.183 kb on + strandat 4442.201 kb on - strandat 4442.225 kb on + strandat 4442.262 kb on + strandat 4442.262 kb on + strandat 4442.264 kb on - strandat 4442.303 kb on + strandat 4442.303 kb on + strandat 4442.312 kb on - strandat 4442.400 kb on + strandat 4442.448 kb on - strandat 4442.448 kb on - strandat 4442.469 kb on + strandat 4442.469 kb on + strandat 4442.512 kb on - strandat 4442.515 kb on + strand, within ytfNat 4442.535 kb on + strand, within ytfNat 4442.535 kb on + strand, within ytfNat 4442.542 kb on - strand, within ytfNat 4442.543 kb on - strand, within ytfNat 4442.544 kb on + strand, within ytfNat 4442.544 kb on + strand, within ytfNat 4442.549 kb on + strand, within ytfNat 4442.557 kb on - strand, within ytfNat 4442.557 kb on - strand, within ytfNat 4442.557 kb on - strand, within ytfNat 4442.571 kb on + strand, within ytfNat 4442.571 kb on + strand, within ytfNat 4442.594 kb on - strand, within ytfNat 4442.634 kb on + strand, within ytfNat 4442.634 kb on + strand, within ytfNat 4442.704 kb on + strand, within ytfNat 4442.704 kb on + strand, within ytfNat 4442.818 kb on - strand, within ytfNat 4442.853 kb on - strand, within ytfNat 4442.853 kb on - strand, within ytfNat 4442.855 kb on + strand, within ytfNat 4442.872 kb on + strand, within ytfNat 4442.890 kb on + strand, within ytfNat 4442.890 kb on + strand, within ytfNat 4442.912 kb on - strand, within ytfNat 4443.017 kb on + strand, within ytfNat 4443.017 kb on + strand, within ytfNat 4443.031 kb on + strand, within ytfNat 4443.033 kb on + strand, within ytfNat 4443.041 kb on - strand, within ytfNat 4443.041 kb on - strand, within ytfNat 4443.041 kb on - strand, within ytfNat 4443.041 kb on - strand, within ytfNat 4443.053 kb on - strand, within ytfNat 4443.053 kb on - strand, within ytfNat 4443.106 kb on + strand, within ytfNat 4443.160 kb on + strand, within ytfNat 4443.212 kb on + strand, within ytfNat 4443.212 kb on + strand, within ytfNat 4443.212 kb on + strand, within ytfNat 4443.233 kb on + strand, within ytfNat 4443.244 kb on + strand, within ytfNat 4443.244 kb on + strand, within ytfNat 4443.244 kb on + strand, within ytfNat 4443.250 kb on + strand, within ytfNat 4443.252 kb on - strand, within ytfNat 4443.266 kb on - strand, within ytfNat 4443.309 kb on - strand, within ytfNat 4443.309 kb on - strand, within ytfNat 4443.309 kb on - strand, within ytfNat 4443.314 kb on + strand, within ytfNat 4443.339 kb on - strand, within ytfNat 4443.339 kb on - strand, within ytfNat 4443.447 kb on - strand, within ytfNat 4443.447 kb on - strand, within ytfNat 4443.452 kb on - strand, within ytfNat 4443.565 kb on - strand, within ytfNat 4443.581 kb on - strand, within ytfNat 4443.698 kb on - strand, within ytfNat 4443.719 kb on - strand, within ytfNat 4443.789 kb on - strand, within ytfNat 4443.793 kb on + strand, within ytfNat 4443.793 kb on + strand, within ytfNat 4443.818 kb on + strand, within ytfNat 4443.820 kb on - strand, within ytfNat 4443.840 kb on + strand, within ytfNat 4444.001 kb on + strand, within ytfNat 4444.015 kb on - strand, within ytfNat 4444.015 kb on - strand, within ytfNat 4444.057 kb on - strand, within ytfNat 4444.057 kb on - strand, within ytfNat 4444.058 kb on - strand, within ytfNat 4444.090 kb on + strand, within ytfNat 4444.090 kb on + strand, within ytfNat 4444.121 kb on + strand, within ytfNat 4444.121 kb on + strand, within ytfNat 4444.194 kb on - strand, within ytfNat 4444.194 kb on - strand, within ytfNat 4444.246 kb on + strand, within ytfNat 4444.246 kb on - strand, within ytfNat 4444.263 kb on + strand, within ytfNat 4444.263 kb on + strand, within ytfNat 4444.349 kb on - strand, within ytfNat 4444.349 kb on - strand, within ytfNat 4444.386 kb on - strand, within ytfNat 4444.386 kb on - strand, within ytfNat 4444.391 kb on - strand, within ytfNat 4444.412 kb on + strand, within ytfNat 4444.420 kb on - strand, within ytfNat 4444.444 kb on + strand, within ytfNat 4444.444 kb on + strand, within ytfNat 4444.510 kb on - strand, within ytfNat 4444.570 kb on - strand, within ytfNat 4444.602 kb on + strand, within ytfNat 4444.602 kb on + strand, within ytfNat 4444.602 kb on + strand, within ytfNat 4444.612 kb on + strand, within ytfNat 4444.612 kb on + strand, within ytfNat 4444.626 kb on - strand, within ytfNat 4444.638 kb on + strand, within ytfNat 4444.653 kb on + strand, within ytfNat 4444.653 kb on + strand, within ytfNat 4444.700 kb on - strand, within ytfNat 4444.800 kb on - strand, within ytfNat 4444.895 kb on + strand, within ytfNat 4444.895 kb on + strand, within ytfNat 4444.898 kb on - strand, within ytfNat 4444.898 kb on - strand, within ytfNat 4444.940 kb on - strand, within ytfNat 4444.991 kb on + strand, within ytfNat 4444.991 kb on + strand, within ytfNat 4445.044 kb on + strand, within ytfNat 4445.044 kb on + strand, within ytfNat 4445.063 kb on - strand, within ytfNat 4445.063 kb on - strand, within ytfNat 4445.065 kb on + strand, within ytfNat 4445.077 kb on - strand, within ytfNat 4445.079 kb on + strand, within ytfNat 4445.081 kb on + strand, within ytfNat 4445.118 kb on + strand, within ytfNat 4445.118 kb on + strand, within ytfNat 4445.141 kb on - strand, within ytfNat 4445.154 kb on + strand, within ytfNat 4445.154 kb on + strand, within ytfNat 4445.237 kb on + strand, within ytfNat 4445.252 kb on - strand, within ytfNat 4445.273 kb on - strand, within ytfNat 4445.320 kb on + strand, within ytfNat 4445.535 kb on - strand, within ytfNat 4445.639 kb on + strandat 4445.668 kb on - strandat 4445.668 kb on - strandat 4445.736 kb on + strandat 4445.737 kb on + strandat 4445.737 kb on + strandat 4445.770 kb on - strandat 4445.794 kb on + strandat 4445.806 kb on + strandat 4445.806 kb on + strandat 4445.806 kb on + strandat 4445.836 kb on + strandat 4445.836 kb on + strandat 4445.859 kb on - strandat 4445.866 kb on + strandat 4445.866 kb on + strandat 4445.962 kb on + strand, within ytfPat 4445.962 kb on + strand, within ytfPat 4446.046 kb on - strand, within ytfPat 4446.131 kb on + strand, within ytfPat 4446.166 kb on + strand, within ytfPat 4446.166 kb on + strand, within ytfPat 4446.166 kb on + strand, within ytfPat 4446.176 kb on + strand, within ytfPat 4446.176 kb on - strand, within ytfPat 4446.176 kb on - strand, within ytfPat 4446.176 kb on - strand, within ytfPat 4446.184 kb on - strand, within ytfPat 4446.184 kb on - strand, within ytfPat 4446.240 kb on + strandat 4446.259 kb on + strandat 4446.260 kb on + strandat 4446.272 kb on - strandat 4446.273 kb on - strandat 4446.273 kb on - strandat 4446.375 kb on - strandat 4446.454 kb on + strandat 4446.454 kb on + strandat 4446.496 kb on + strand, within chpSat 4446.501 kb on + strand, within chpSat 4446.501 kb on + strand, within chpSat 4446.509 kb on - strand, within chpSat 4446.509 kb on - strand, within chpSat 4446.514 kb on - strand, within chpSat 4446.559 kb on + strand, within chpSat 4446.569 kb on - strand, within chpSat 4446.569 kb on - strand, within chpSat 4446.646 kb on - strand, within chpSat 4446.652 kb on - strand, within chpSat 4446.746 kb on + strandat 4446.777 kb on + strand, within chpBat 4446.787 kb on + strand, within chpBat 4446.787 kb on + strand, within chpBat 4446.787 kb on - strand, within chpBat 4446.787 kb on - strand, within chpBat 4446.794 kb on + strand, within chpBat 4446.910 kb on + strand, within chpB

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 20mM
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4,441,144 - ytfM b4220 0.43 +0.9
4,441,234 - ytfM b4220 0.48 +0.7
4,441,234 - ytfM b4220 0.48 +0.0
4,441,292 + ytfM b4220 0.51 +0.7
4,441,306 + ytfM b4220 0.52 +0.6
4,441,382 + ytfM b4220 0.56 +0.1
4,441,406 + ytfM b4220 0.58 +0.9
4,441,406 + ytfM b4220 0.58 +1.1
4,441,416 + ytfM b4220 0.58 +1.2
4,441,424 - ytfM b4220 0.59 +1.2
4,441,441 + ytfM b4220 0.60 +3.0
4,441,441 + ytfM b4220 0.60 +1.9
4,441,442 + ytfM b4220 0.60 +1.1
4,441,456 + ytfM b4220 0.61 +1.1
4,441,458 + ytfM b4220 0.61 +0.7
4,441,495 + ytfM b4220 0.63 -0.3
4,441,503 - ytfM b4220 0.63 +0.7
4,441,521 - ytfM b4220 0.64 -0.5
4,441,559 + ytfM b4220 0.67 +1.4
4,441,564 + ytfM b4220 0.67 +1.3
4,441,564 + ytfM b4220 0.67 +0.3
4,441,567 - ytfM b4220 0.67 +0.5
4,441,614 + ytfM b4220 0.70 +0.9
4,441,614 + ytfM b4220 0.70 -0.0
4,441,639 + ytfM b4220 0.71 +1.8
4,441,639 + ytfM b4220 0.71 -1.5
4,441,653 + ytfM b4220 0.72 +0.6
4,441,657 - ytfM b4220 0.72 +0.3
4,441,657 - ytfM b4220 0.72 +1.0
4,441,657 - ytfM b4220 0.72 +0.1
4,441,717 + ytfM b4220 0.76 +1.0
4,441,727 - ytfM b4220 0.76 -0.1
4,441,828 + ytfM b4220 0.82 +0.8
4,441,933 - ytfM b4220 0.88 +0.4
4,441,933 - ytfM b4220 0.88 +1.1
4,441,969 - -0.3
4,441,969 - +0.5
4,442,033 - +1.0
4,442,108 + +2.0
4,442,183 + +0.8
4,442,201 - +2.4
4,442,225 + +0.7
4,442,262 + +0.6
4,442,262 + -0.6
4,442,264 - +0.5
4,442,303 + +0.5
4,442,303 + +1.1
4,442,312 - +1.1
4,442,400 + +0.8
4,442,448 - +0.7
4,442,448 - -1.0
4,442,469 + +0.7
4,442,469 + +0.9
4,442,512 - +1.0
4,442,515 + ytfN b4221 0.10 +0.3
4,442,535 + ytfN b4221 0.11 +1.6
4,442,535 + ytfN b4221 0.11 +0.8
4,442,542 - ytfN b4221 0.11 +1.0
4,442,543 - ytfN b4221 0.11 +1.2
4,442,544 + ytfN b4221 0.11 +0.8
4,442,544 + ytfN b4221 0.11 +0.6
4,442,549 + ytfN b4221 0.11 +1.1
4,442,557 - ytfN b4221 0.11 -0.4
4,442,557 - ytfN b4221 0.11 +1.1
4,442,557 - ytfN b4221 0.11 +1.5
4,442,571 + ytfN b4221 0.12 +1.0
4,442,571 + ytfN b4221 0.12 +1.5
4,442,594 - ytfN b4221 0.12 +0.4
4,442,634 + ytfN b4221 0.13 +0.6
4,442,634 + ytfN b4221 0.13 +0.8
4,442,704 + ytfN b4221 0.15 -0.3
4,442,704 + ytfN b4221 0.15 +0.5
4,442,818 - ytfN b4221 0.18 +0.1
4,442,853 - ytfN b4221 0.19 +0.2
4,442,853 - ytfN b4221 0.19 +0.7
4,442,855 + ytfN b4221 0.19 +0.9
4,442,872 + ytfN b4221 0.19 +2.1
4,442,890 + ytfN b4221 0.20 +1.3
4,442,890 + ytfN b4221 0.20 +0.9
4,442,912 - ytfN b4221 0.21 +0.2
4,443,017 + ytfN b4221 0.23 +2.1
4,443,017 + ytfN b4221 0.23 +1.3
4,443,031 + ytfN b4221 0.24 +0.4
4,443,033 + ytfN b4221 0.24 +0.4
4,443,041 - ytfN b4221 0.24 +1.2
4,443,041 - ytfN b4221 0.24 +0.1
4,443,041 - ytfN b4221 0.24 -0.7
4,443,041 - ytfN b4221 0.24 +1.8
4,443,053 - ytfN b4221 0.24 +0.6
4,443,053 - ytfN b4221 0.24 -2.5
4,443,106 + ytfN b4221 0.26 +0.5
4,443,160 + ytfN b4221 0.27 +0.7
4,443,212 + ytfN b4221 0.28 +0.8
4,443,212 + ytfN b4221 0.28 -0.0
4,443,212 + ytfN b4221 0.28 +1.4
4,443,233 + ytfN b4221 0.29 +0.2
4,443,244 + ytfN b4221 0.29 +0.8
4,443,244 + ytfN b4221 0.29 +0.1
4,443,244 + ytfN b4221 0.29 +1.4
4,443,250 + ytfN b4221 0.29 +1.3
4,443,252 - ytfN b4221 0.30 +1.8
4,443,266 - ytfN b4221 0.30 +1.0
4,443,309 - ytfN b4221 0.31 +1.0
4,443,309 - ytfN b4221 0.31 +0.4
4,443,309 - ytfN b4221 0.31 +0.9
4,443,314 + ytfN b4221 0.31 +1.2
4,443,339 - ytfN b4221 0.32 +0.7
4,443,339 - ytfN b4221 0.32 +0.7
4,443,447 - ytfN b4221 0.35 +0.6
4,443,447 - ytfN b4221 0.35 +0.9
4,443,452 - ytfN b4221 0.35 +0.7
4,443,565 - ytfN b4221 0.38 +0.6
4,443,581 - ytfN b4221 0.38 -2.7
4,443,698 - ytfN b4221 0.41 -0.6
4,443,719 - ytfN b4221 0.42 +0.6
4,443,789 - ytfN b4221 0.44 +1.0
4,443,793 + ytfN b4221 0.44 -0.4
4,443,793 + ytfN b4221 0.44 -0.4
4,443,818 + ytfN b4221 0.45 +1.1
4,443,820 - ytfN b4221 0.45 +1.2
4,443,840 + ytfN b4221 0.45 +0.8
4,444,001 + ytfN b4221 0.49 +1.4
4,444,015 - ytfN b4221 0.50 +0.2
4,444,015 - ytfN b4221 0.50 +0.9
4,444,057 - ytfN b4221 0.51 -0.4
4,444,057 - ytfN b4221 0.51 +0.8
4,444,058 - ytfN b4221 0.51 +0.3
4,444,090 + ytfN b4221 0.52 +0.8
4,444,090 + ytfN b4221 0.52 +2.2
4,444,121 + ytfN b4221 0.53 +1.5
4,444,121 + ytfN b4221 0.53 +1.3
4,444,194 - ytfN b4221 0.54 +1.3
4,444,194 - ytfN b4221 0.54 -0.2
4,444,246 + ytfN b4221 0.56 +1.0
4,444,246 - ytfN b4221 0.56 +2.8
4,444,263 + ytfN b4221 0.56 +1.6
4,444,263 + ytfN b4221 0.56 +2.3
4,444,349 - ytfN b4221 0.59 +0.2
4,444,349 - ytfN b4221 0.59 +0.3
4,444,386 - ytfN b4221 0.60 +0.5
4,444,386 - ytfN b4221 0.60 +1.6
4,444,391 - ytfN b4221 0.60 -0.5
4,444,412 + ytfN b4221 0.60 +1.1
4,444,420 - ytfN b4221 0.60 +0.9
4,444,444 + ytfN b4221 0.61 +0.1
4,444,444 + ytfN b4221 0.61 -1.1
4,444,510 - ytfN b4221 0.63 +0.8
4,444,570 - ytfN b4221 0.64 +0.4
4,444,602 + ytfN b4221 0.65 -0.3
4,444,602 + ytfN b4221 0.65 +0.7
4,444,602 + ytfN b4221 0.65 +0.5
4,444,612 + ytfN b4221 0.66 +0.6
4,444,612 + ytfN b4221 0.66 +0.4
4,444,626 - ytfN b4221 0.66 +0.5
4,444,638 + ytfN b4221 0.66 +1.2
4,444,653 + ytfN b4221 0.67 +0.6
4,444,653 + ytfN b4221 0.67 +1.0
4,444,700 - ytfN b4221 0.68 -0.4
4,444,800 - ytfN b4221 0.71 +0.4
4,444,895 + ytfN b4221 0.73 +0.5
4,444,895 + ytfN b4221 0.73 +1.3
4,444,898 - ytfN b4221 0.73 +1.5
4,444,898 - ytfN b4221 0.73 +2.1
4,444,940 - ytfN b4221 0.74 +0.3
4,444,991 + ytfN b4221 0.76 +0.6
4,444,991 + ytfN b4221 0.76 +1.7
4,445,044 + ytfN b4221 0.77 +1.3
4,445,044 + ytfN b4221 0.77 +1.5
4,445,063 - ytfN b4221 0.77 -0.0
4,445,063 - ytfN b4221 0.77 +2.2
4,445,065 + ytfN b4221 0.78 +1.4
4,445,077 - ytfN b4221 0.78 -0.1
4,445,079 + ytfN b4221 0.78 +1.5
4,445,081 + ytfN b4221 0.78 -0.6
4,445,118 + ytfN b4221 0.79 +1.6
4,445,118 + ytfN b4221 0.79 +0.9
4,445,141 - ytfN b4221 0.80 +1.4
4,445,154 + ytfN b4221 0.80 +1.5
4,445,154 + ytfN b4221 0.80 +0.5
4,445,237 + ytfN b4221 0.82 -0.5
4,445,252 - ytfN b4221 0.82 +0.5
4,445,273 - ytfN b4221 0.83 +1.0
4,445,320 + ytfN b4221 0.84 +0.9
4,445,535 - ytfN b4221 0.90 +0.7
4,445,639 + +0.5
4,445,668 - -0.2
4,445,668 - -0.0
4,445,736 + +1.2
4,445,737 + +1.9
4,445,737 + +2.3
4,445,770 - +1.0
4,445,794 + +0.4
4,445,806 + +0.8
4,445,806 + -0.9
4,445,806 + +0.2
4,445,836 + +1.0
4,445,836 + +0.8
4,445,859 - +1.4
4,445,866 + +1.9
4,445,866 + +1.0
4,445,962 + ytfP b4222 0.13 +0.2
4,445,962 + ytfP b4222 0.13 +0.1
4,446,046 - ytfP b4222 0.38 -0.4
4,446,131 + ytfP b4222 0.63 -1.5
4,446,166 + ytfP b4222 0.73 -1.8
4,446,166 + ytfP b4222 0.73 +0.5
4,446,166 + ytfP b4222 0.73 +0.9
4,446,176 + ytfP b4222 0.76 -0.7
4,446,176 - ytfP b4222 0.76 -0.6
4,446,176 - ytfP b4222 0.76 +1.4
4,446,176 - ytfP b4222 0.76 +0.9
4,446,184 - ytfP b4222 0.78 +0.2
4,446,184 - ytfP b4222 0.78 -0.5
4,446,240 + +0.5
4,446,259 + -0.9
4,446,260 + +0.6
4,446,272 - -1.3
4,446,273 - -0.8
4,446,273 - -3.1
4,446,375 - -0.7
4,446,454 + +0.1
4,446,454 + -0.1
4,446,496 + chpS b4224 0.10 +0.4
4,446,501 + chpS b4224 0.12 +0.5
4,446,501 + chpS b4224 0.12 +0.5
4,446,509 - chpS b4224 0.15 +1.3
4,446,509 - chpS b4224 0.15 -2.3
4,446,514 - chpS b4224 0.17 -2.6
4,446,559 + chpS b4224 0.35 +2.2
4,446,569 - chpS b4224 0.39 +1.2
4,446,569 - chpS b4224 0.39 -0.2
4,446,646 - chpS b4224 0.70 +3.8
4,446,652 - chpS b4224 0.72 -0.7
4,446,746 + +0.5
4,446,777 + chpB b4225 0.18 -0.1
4,446,787 + chpB b4225 0.21 -0.2
4,446,787 + chpB b4225 0.21 -0.1
4,446,787 - chpB b4225 0.21 +1.5
4,446,787 - chpB b4225 0.21 -0.2
4,446,794 + chpB b4225 0.23 -1.3
4,446,910 + chpB b4225 0.56 -1.0

Or see this region's nucleotide sequence