Strain Fitness in Escherichia coli BW25113 around b3550
Experiment: carbon source D-Mannose 20mM
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | carbon source D-Mannose 20mM |
---|---|---|---|---|---|
remove | |||||
3,710,694 | - | yhjY | b3548 | 0.62 | -2.0 |
3,710,703 | - | yhjY | b3548 | 0.64 | +0.2 |
3,710,729 | + | yhjY | b3548 | 0.67 | -0.8 |
3,710,732 | - | yhjY | b3548 | 0.68 | -0.1 |
3,710,779 | - | yhjY | b3548 | 0.74 | -0.7 |
3,710,797 | - | yhjY | b3548 | 0.77 | -1.6 |
3,710,798 | - | yhjY | b3548 | 0.77 | +0.5 |
3,710,799 | - | yhjY | b3548 | 0.77 | -0.3 |
3,710,800 | + | yhjY | b3548 | 0.77 | -1.3 |
3,710,800 | + | yhjY | b3548 | 0.77 | +0.9 |
3,710,800 | + | yhjY | b3548 | 0.77 | +0.4 |
3,710,805 | + | yhjY | b3548 | 0.78 | +1.3 |
3,710,805 | + | yhjY | b3548 | 0.78 | +1.2 |
3,710,805 | + | yhjY | b3548 | 0.78 | +1.0 |
3,710,805 | + | yhjY | b3548 | 0.78 | +0.0 |
3,710,820 | - | yhjY | b3548 | 0.80 | -5.2 |
3,710,859 | - | yhjY | b3548 | 0.86 | +0.3 |
3,710,868 | + | yhjY | b3548 | 0.87 | -0.1 |
3,710,917 | + | +0.7 | |||
3,710,919 | + | -2.5 | |||
3,710,966 | + | +1.1 | |||
3,710,966 | + | -3.2 | |||
3,710,966 | + | -1.1 | |||
3,710,966 | - | +2.1 | |||
3,710,966 | - | +3.2 | |||
3,710,974 | - | +0.2 | |||
3,711,005 | + | -0.3 | |||
3,711,005 | + | -0.3 | |||
3,711,010 | - | -2.0 | |||
3,711,011 | - | +0.8 | |||
3,711,019 | - | -0.2 | |||
3,711,042 | - | +1.0 | |||
3,711,091 | + | -0.1 | |||
3,711,123 | + | -0.9 | |||
3,711,137 | + | -1.4 | |||
3,711,137 | + | +1.1 | |||
3,711,155 | - | -0.7 | |||
3,711,162 | + | +1.7 | |||
3,711,162 | + | -0.1 | |||
3,711,183 | + | tag | b3549 | 0.12 | -0.6 |
3,711,183 | + | tag | b3549 | 0.12 | -0.2 |
3,711,229 | + | tag | b3549 | 0.20 | +0.4 |
3,711,298 | - | tag | b3549 | 0.32 | +0.2 |
3,711,298 | - | -0.1 | |||
3,711,352 | + | tag | b3549 | 0.42 | -0.6 |
3,711,357 | - | tag | b3549 | 0.43 | -0.8 |
3,711,357 | - | tag | b3549 | 0.43 | +0.2 |
3,711,360 | - | tag | b3549 | 0.43 | -3.8 |
3,711,360 | - | tag | b3549 | 0.43 | +0.4 |
3,711,421 | - | tag | b3549 | 0.54 | +0.3 |
3,711,433 | - | tag | b3549 | 0.56 | +0.0 |
3,711,490 | - | tag | b3549 | 0.66 | -1.0 |
3,711,498 | + | tag | b3549 | 0.68 | -0.0 |
3,711,500 | - | tag | b3549 | 0.68 | +0.8 |
3,711,534 | - | tag | b3549 | 0.74 | -0.8 |
3,711,534 | - | tag | b3549 | 0.74 | +0.1 |
3,711,613 | - | tag | b3549 | 0.88 | -1.1 |
3,711,613 | - | tag | b3549 | 0.88 | -0.6 |
3,711,617 | + | tag | b3549 | 0.89 | +0.4 |
3,711,617 | + | tag | b3549 | 0.89 | +1.0 |
3,711,617 | + | tag | b3549 | 0.89 | -0.1 |
3,711,625 | - | -0.6 | |||
3,711,635 | - | +2.0 | |||
3,711,645 | - | -0.2 | |||
3,711,645 | - | +0.2 | |||
3,711,648 | + | +0.2 | |||
3,711,702 | - | +0.3 | |||
3,711,739 | - | yiaC | b3550 | 0.15 | +0.6 |
3,711,751 | + | yiaC | b3550 | 0.17 | +0.1 |
3,711,751 | + | yiaC | b3550 | 0.17 | +0.9 |
3,711,786 | - | yiaC | b3550 | 0.25 | +0.1 |
3,711,873 | - | yiaC | b3550 | 0.45 | +0.4 |
3,711,873 | - | yiaC | b3550 | 0.45 | -0.5 |
3,711,873 | - | yiaC | b3550 | 0.45 | +0.8 |
3,711,874 | - | yiaC | b3550 | 0.45 | -1.3 |
3,711,923 | - | yiaC | b3550 | 0.56 | -1.3 |
3,711,946 | + | yiaC | b3550 | 0.61 | +0.8 |
3,711,946 | + | yiaC | b3550 | 0.61 | -0.7 |
3,711,949 | + | yiaC | b3550 | 0.62 | +0.4 |
3,711,954 | - | yiaC | b3550 | 0.63 | -1.2 |
3,711,954 | - | yiaC | b3550 | 0.63 | -3.6 |
3,711,970 | + | yiaC | b3550 | 0.67 | +0.4 |
3,711,973 | - | yiaC | b3550 | 0.68 | +0.4 |
3,712,058 | + | yiaC | b3550 | 0.87 | +2.0 |
3,712,073 | + | +0.5 | |||
3,712,116 | - | +1.5 | |||
3,712,118 | - | -0.7 | |||
3,712,118 | - | +0.2 | |||
3,712,135 | + | -0.6 | |||
3,712,135 | + | -0.4 | |||
3,712,174 | + | -0.8 | |||
3,712,191 | + | -1.1 | |||
3,712,191 | + | +0.3 | |||
3,712,236 | - | -0.2 | |||
3,712,236 | - | -1.1 | |||
3,712,305 | + | +0.3 | |||
3,712,320 | + | bisC | b3551 | 0.10 | -2.1 |
3,712,320 | + | bisC | b3551 | 0.10 | -0.2 |
3,712,349 | + | bisC | b3551 | 0.11 | -0.6 |
3,712,357 | - | bisC | b3551 | 0.12 | -0.3 |
3,712,364 | + | bisC | b3551 | 0.12 | +0.9 |
3,712,364 | + | bisC | b3551 | 0.12 | -3.7 |
3,712,374 | - | bisC | b3551 | 0.12 | +0.8 |
3,712,374 | - | bisC | b3551 | 0.12 | +0.5 |
3,712,382 | + | bisC | b3551 | 0.13 | +0.1 |
3,712,405 | - | -1.7 | |||
3,712,408 | + | bisC | b3551 | 0.14 | -0.7 |
3,712,411 | - | bisC | b3551 | 0.14 | +1.3 |
3,712,418 | + | bisC | b3551 | 0.14 | +0.0 |
3,712,441 | + | bisC | b3551 | 0.15 | -0.2 |
3,712,459 | + | bisC | b3551 | 0.16 | +0.5 |
3,712,462 | - | bisC | b3551 | 0.16 | +0.2 |
3,712,462 | - | bisC | b3551 | 0.16 | +1.2 |
3,712,487 | + | bisC | b3551 | 0.17 | +0.0 |
3,712,494 | - | bisC | b3551 | 0.18 | +0.3 |
3,712,502 | - | bisC | b3551 | 0.18 | +1.7 |
3,712,502 | - | bisC | b3551 | 0.18 | +0.3 |
3,712,564 | - | bisC | b3551 | 0.21 | -0.6 |
3,712,565 | - | bisC | b3551 | 0.21 | +0.3 |
3,712,566 | + | bisC | b3551 | 0.21 | +0.1 |
3,712,566 | + | bisC | b3551 | 0.21 | +0.0 |
3,712,574 | - | bisC | b3551 | 0.21 | +0.4 |
3,712,579 | - | bisC | b3551 | 0.21 | -0.5 |
3,712,584 | - | bisC | b3551 | 0.21 | +0.9 |
3,712,595 | + | bisC | b3551 | 0.22 | +0.5 |
3,712,879 | - | bisC | b3551 | 0.34 | +2.6 |
3,712,895 | + | bisC | b3551 | 0.35 | -2.3 |
3,712,970 | + | bisC | b3551 | 0.38 | +1.1 |
3,712,970 | + | bisC | b3551 | 0.38 | +0.6 |
3,712,970 | + | bisC | b3551 | 0.38 | +0.4 |
3,713,000 | - | bisC | b3551 | 0.39 | -2.0 |
3,713,018 | + | bisC | b3551 | 0.40 | -4.1 |
3,713,026 | - | bisC | b3551 | 0.40 | +1.0 |
3,713,042 | + | bisC | b3551 | 0.41 | -1.1 |
3,713,110 | - | bisC | b3551 | 0.44 | +1.1 |
3,713,110 | - | bisC | b3551 | 0.44 | +0.6 |
3,713,115 | + | bisC | b3551 | 0.44 | +0.7 |
3,713,115 | + | bisC | b3551 | 0.44 | +0.3 |
Or see this region's nucleotide sequence