Strain Fitness in Escherichia coli BW25113 around b1677

Experiment: carbon source D-Mannose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpykF and lpp are separated by 310 nucleotideslpp and ynhG are separated by 63 nucleotides b1676: pykF - pyruvate kinase (NCBI), at 1,753,722 to 1,755,134 pykF b1677: lpp - murein lipoprotein (NCBI), at 1,755,445 to 1,755,681 lpp b1678: ynhG - hypothetical protein (NCBI), at 1,755,745 to 1,756,749 ynhG Position (kb) 1755 1756Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1754.581 kb on + strand, within pykFat 1754.581 kb on + strand, within pykFat 1754.992 kb on + strand, within pykFat 1755.104 kb on + strandat 1755.375 kb on + strandat 1755.410 kb on + strandat 1755.417 kb on + strandat 1755.476 kb on - strand, within lppat 1755.478 kb on + strand, within lppat 1755.485 kb on + strand, within lppat 1755.576 kb on + strand, within lppat 1755.662 kb on + strandat 1755.782 kb on - strandat 1755.985 kb on - strand, within ynhGat 1755.985 kb on - strand, within ynhGat 1756.008 kb on + strand, within ynhGat 1756.008 kb on + strand, within ynhGat 1756.016 kb on + strand, within ynhGat 1756.016 kb on - strand, within ynhGat 1756.016 kb on - strand, within ynhGat 1756.080 kb on + strand, within ynhGat 1756.080 kb on + strand, within ynhGat 1756.151 kb on - strand, within ynhGat 1756.195 kb on - strand, within ynhGat 1756.211 kb on + strand, within ynhGat 1756.285 kb on - strand, within ynhGat 1756.285 kb on - strand, within ynhGat 1756.445 kb on - strand, within ynhGat 1756.470 kb on + strand, within ynhGat 1756.612 kb on + strand, within ynhGat 1756.620 kb on - strand, within ynhGat 1756.620 kb on - strand, within ynhG

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 20mM
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1,754,581 + pykF b1676 0.61 +0.2
1,754,581 + pykF b1676 0.61 +0.3
1,754,992 + pykF b1676 0.90 +1.6
1,755,104 + +1.8
1,755,375 + +3.1
1,755,410 + +2.9
1,755,417 + +2.6
1,755,476 - lpp b1677 0.13 +2.9
1,755,478 + lpp b1677 0.14 +2.7
1,755,485 + lpp b1677 0.17 +2.8
1,755,576 + lpp b1677 0.55 +1.7
1,755,662 + +1.3
1,755,782 - +0.6
1,755,985 - ynhG b1678 0.24 -0.3
1,755,985 - ynhG b1678 0.24 +0.1
1,756,008 + ynhG b1678 0.26 -2.7
1,756,008 + ynhG b1678 0.26 -2.4
1,756,016 + ynhG b1678 0.27 -0.0
1,756,016 - ynhG b1678 0.27 +0.6
1,756,016 - ynhG b1678 0.27 +0.6
1,756,080 + ynhG b1678 0.33 -0.3
1,756,080 + ynhG b1678 0.33 -4.6
1,756,151 - ynhG b1678 0.40 +0.8
1,756,195 - ynhG b1678 0.45 -0.4
1,756,211 + ynhG b1678 0.46 +2.2
1,756,285 - ynhG b1678 0.54 +0.1
1,756,285 - ynhG b1678 0.54 +0.0
1,756,445 - ynhG b1678 0.70 +0.4
1,756,470 + ynhG b1678 0.72 +0.8
1,756,612 + ynhG b1678 0.86 -0.8
1,756,620 - ynhG b1678 0.87 +0.8
1,756,620 - ynhG b1678 0.87 -0.3

Or see this region's nucleotide sequence