Strain Fitness in Escherichia coli BW25113 around b1651

Experiment: carbon source D-Mannose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnemA and gloA are separated by 80 nucleotidesgloA and rnt are separated by 102 nucleotidesrnt and lhr are separated by 92 nucleotides b1650: nemA - N-ethylmaleimide reductase, FMN-linked (NCBI), at 1,724,683 to 1,725,780 nemA b1651: gloA - glyoxalase I, Ni-dependent (NCBI), at 1,725,861 to 1,726,268 gloA b1652: rnt - ribonuclease T (NCBI), at 1,726,371 to 1,727,018 rnt b1653: lhr - predicted ATP-dependent helicase (NCBI), at 1,727,111 to 1,731,727 lhr Position (kb) 1725 1726 1727Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1725.072 kb on + strand, within nemAat 1725.075 kb on - strand, within nemAat 1725.075 kb on - strand, within nemAat 1725.118 kb on - strand, within nemAat 1725.233 kb on - strand, within nemAat 1725.233 kb on - strand, within nemAat 1725.403 kb on + strand, within nemAat 1725.437 kb on + strand, within nemAat 1725.754 kb on + strandat 1725.761 kb on - strandat 1725.763 kb on - strandat 1725.763 kb on - strandat 1725.877 kb on - strandat 1725.877 kb on - strandat 1726.040 kb on - strand, within gloAat 1726.072 kb on - strand, within gloAat 1726.092 kb on + strand, within gloAat 1726.185 kb on - strand, within gloAat 1726.185 kb on - strand, within gloA

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 20mM
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1,725,072 + nemA b1650 0.35 +1.5
1,725,075 - nemA b1650 0.36 +0.4
1,725,075 - nemA b1650 0.36 -3.4
1,725,118 - nemA b1650 0.40 +0.1
1,725,233 - nemA b1650 0.50 +1.3
1,725,233 - nemA b1650 0.50 +1.8
1,725,403 + nemA b1650 0.66 +0.2
1,725,437 + nemA b1650 0.69 -0.1
1,725,754 + -0.8
1,725,761 - +0.4
1,725,763 - +0.7
1,725,763 - +0.3
1,725,877 - +0.6
1,725,877 - +0.2
1,726,040 - gloA b1651 0.44 +0.6
1,726,072 - gloA b1651 0.52 +1.2
1,726,092 + gloA b1651 0.57 -0.3
1,726,185 - gloA b1651 0.79 +0.5
1,726,185 - gloA b1651 0.79 +2.4

Or see this region's nucleotide sequence