Strain Fitness in Escherichia coli BW25113 around b0853

Experiment: carbon source D-Mannose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrimK and ybjN are separated by 87 nucleotidesybjN and potF are separated by 350 nucleotides b0852: rimK - ribosomal protein S6 modification protein (NCBI), at 891,190 to 892,092 rimK b0853: ybjN - predicted oxidoreductase (NCBI), at 892,180 to 892,656 ybjN b0854: potF - putrescine transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 893,007 to 894,119 potF Position (kb) 892 893Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 891.213 kb on + strandat 891.222 kb on + strandat 891.243 kb on + strandat 891.251 kb on + strandat 891.251 kb on + strandat 891.251 kb on + strandat 891.251 kb on + strandat 891.254 kb on + strandat 891.387 kb on - strand, within rimKat 891.387 kb on - strand, within rimKat 891.703 kb on - strand, within rimKat 891.703 kb on - strand, within rimKat 891.766 kb on - strand, within rimKat 891.868 kb on - strand, within rimKat 892.020 kb on - strandat 892.167 kb on + strandat 892.206 kb on + strandat 892.244 kb on - strand, within ybjNat 892.284 kb on - strand, within ybjNat 892.284 kb on - strand, within ybjNat 892.304 kb on + strand, within ybjNat 892.389 kb on - strand, within ybjNat 892.389 kb on - strand, within ybjNat 892.411 kb on + strand, within ybjNat 892.411 kb on + strand, within ybjNat 892.524 kb on + strand, within ybjNat 892.550 kb on - strand, within ybjNat 892.609 kb on - strandat 892.609 kb on - strandat 893.167 kb on + strand, within potFat 893.261 kb on + strand, within potFat 893.352 kb on - strand, within potFat 893.362 kb on - strand, within potFat 893.400 kb on + strand, within potFat 893.408 kb on - strand, within potFat 893.408 kb on - strand, within potFat 893.490 kb on - strand, within potF

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 20mM
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891,213 + +0.6
891,222 + +0.2
891,243 + +1.3
891,251 + +2.8
891,251 + +0.4
891,251 + +1.5
891,251 + -0.9
891,254 + +1.0
891,387 - rimK b0852 0.22 +1.0
891,387 - rimK b0852 0.22 +1.2
891,703 - rimK b0852 0.57 +0.9
891,703 - rimK b0852 0.57 +1.1
891,766 - rimK b0852 0.64 +0.7
891,868 - rimK b0852 0.75 +0.8
892,020 - +1.1
892,167 + -0.6
892,206 + +0.4
892,244 - ybjN b0853 0.13 +0.2
892,284 - ybjN b0853 0.22 +2.6
892,284 - ybjN b0853 0.22 +3.9
892,304 + ybjN b0853 0.26 +0.0
892,389 - ybjN b0853 0.44 +0.8
892,389 - ybjN b0853 0.44 +0.8
892,411 + ybjN b0853 0.48 -0.4
892,411 + ybjN b0853 0.48 +0.3
892,524 + ybjN b0853 0.72 +0.3
892,550 - ybjN b0853 0.78 -0.9
892,609 - +0.7
892,609 - -2.2
893,167 + potF b0854 0.14 +1.6
893,261 + potF b0854 0.23 -0.6
893,352 - potF b0854 0.31 -1.9
893,362 - potF b0854 0.32 +0.2
893,400 + potF b0854 0.35 -0.4
893,408 - potF b0854 0.36 +0.1
893,408 - potF b0854 0.36 +1.1
893,490 - potF b0854 0.43 -0.2

Or see this region's nucleotide sequence