Strain Fitness in Escherichia coli BW25113 around b3935

Experiment: carbon source D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcytR and priA are separated by 155 nucleotidespriA and rpmE are separated by 202 nucleotidesrpmE and yiiX are separated by 60 nucleotides b3934: cytR - DNA-binding transcriptional dual regulator (NCBI), at 4,121,454 to 4,122,479 cytR b3935: priA - primosome assembly protein PriA (NCBI), at 4,122,635 to 4,124,833 priA b3936: rpmE - 50S ribosomal subunit protein L31 (NCBI), at 4,125,036 to 4,125,248 rpmE b3937: yiiX - predicted peptidoglycan peptidase (NCBI), at 4,125,309 to 4,125,917 yiiX Position (kb) 4122 4123 4124 4125Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 4121.714 kb on + strand, within cytRat 4121.714 kb on + strand, within cytRat 4121.818 kb on - strand, within cytRat 4121.818 kb on - strand, within cytRat 4121.827 kb on - strand, within cytRat 4121.847 kb on + strand, within cytRat 4121.847 kb on + strand, within cytRat 4121.852 kb on + strand, within cytRat 4121.858 kb on + strand, within cytRat 4121.895 kb on + strand, within cytRat 4121.895 kb on + strand, within cytRat 4121.905 kb on - strand, within cytRat 4121.906 kb on + strand, within cytRat 4121.914 kb on - strand, within cytRat 4121.923 kb on + strand, within cytRat 4121.970 kb on + strand, within cytRat 4121.982 kb on + strand, within cytRat 4121.987 kb on + strand, within cytRat 4121.989 kb on + strand, within cytRat 4121.990 kb on - strand, within cytRat 4121.990 kb on - strand, within cytRat 4122.053 kb on - strand, within cytRat 4122.053 kb on - strand, within cytRat 4122.088 kb on - strand, within cytRat 4122.098 kb on - strand, within cytRat 4122.104 kb on - strand, within cytRat 4122.121 kb on - strand, within cytRat 4122.185 kb on - strand, within cytRat 4122.190 kb on - strand, within cytRat 4122.190 kb on - strand, within cytRat 4122.203 kb on + strand, within cytRat 4122.247 kb on + strand, within cytRat 4122.259 kb on + strand, within cytRat 4122.277 kb on - strand, within cytRat 4122.277 kb on - strand, within cytRat 4122.312 kb on - strand, within cytRat 4122.353 kb on + strand, within cytRat 4122.353 kb on + strand, within cytRat 4122.361 kb on - strand, within cytRat 4122.364 kb on + strand, within cytRat 4122.387 kb on + strandat 4122.395 kb on - strandat 4122.499 kb on + strandat 4122.502 kb on + strandat 4122.647 kb on - strandat 4124.262 kb on + strand, within priAat 4124.288 kb on - strand, within priAat 4124.669 kb on - strandat 4124.669 kb on - strandat 4124.791 kb on - strandat 4124.791 kb on - strandat 4124.835 kb on + strandat 4124.839 kb on + strandat 4124.865 kb on + strandat 4124.878 kb on + strandat 4124.886 kb on - strandat 4124.896 kb on - strandat 4124.959 kb on + strandat 4125.289 kb on - strandat 4125.289 kb on - strandat 4125.381 kb on - strand, within yiiXat 4125.381 kb on - strand, within yiiXat 4125.485 kb on - strand, within yiiXat 4125.494 kb on + strand, within yiiXat 4125.515 kb on + strand, within yiiXat 4125.527 kb on + strand, within yiiXat 4125.527 kb on + strand, within yiiXat 4125.527 kb on + strand, within yiiXat 4125.527 kb on + strand, within yiiXat 4125.537 kb on + strand, within yiiXat 4125.537 kb on + strand, within yiiXat 4125.545 kb on - strand, within yiiXat 4125.545 kb on - strand, within yiiXat 4125.631 kb on - strand, within yiiXat 4125.647 kb on - strand, within yiiXat 4125.661 kb on + strand, within yiiXat 4125.685 kb on + strand, within yiiXat 4125.690 kb on + strand, within yiiXat 4125.713 kb on - strand, within yiiXat 4125.713 kb on - strand, within yiiXat 4125.758 kb on + strand, within yiiXat 4125.768 kb on - strand, within yiiXat 4125.784 kb on + strand, within yiiXat 4125.791 kb on - strand, within yiiXat 4125.792 kb on - strand, within yiiXat 4125.792 kb on - strand, within yiiX

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 20mM
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4,121,714 + cytR b3934 0.25 +1.8
4,121,714 + cytR b3934 0.25 -0.3
4,121,818 - cytR b3934 0.35 +1.1
4,121,818 - cytR b3934 0.35 -1.5
4,121,827 - cytR b3934 0.36 +0.7
4,121,847 + cytR b3934 0.38 +1.0
4,121,847 + cytR b3934 0.38 +0.0
4,121,852 + cytR b3934 0.39 -0.7
4,121,858 + cytR b3934 0.39 -0.4
4,121,895 + cytR b3934 0.43 +0.4
4,121,895 + cytR b3934 0.43 -1.7
4,121,905 - cytR b3934 0.44 +1.0
4,121,906 + cytR b3934 0.44 -0.1
4,121,914 - cytR b3934 0.45 +1.5
4,121,923 + cytR b3934 0.46 -1.4
4,121,970 + cytR b3934 0.50 -0.2
4,121,982 + cytR b3934 0.51 +1.2
4,121,987 + cytR b3934 0.52 -3.5
4,121,989 + cytR b3934 0.52 +0.6
4,121,990 - cytR b3934 0.52 +1.8
4,121,990 - cytR b3934 0.52 +1.2
4,122,053 - cytR b3934 0.58 +0.4
4,122,053 - cytR b3934 0.58 -3.7
4,122,088 - cytR b3934 0.62 +0.6
4,122,098 - cytR b3934 0.63 -1.1
4,122,104 - cytR b3934 0.63 +0.4
4,122,121 - cytR b3934 0.65 -0.7
4,122,185 - cytR b3934 0.71 +0.7
4,122,190 - cytR b3934 0.72 -0.6
4,122,190 - cytR b3934 0.72 +0.2
4,122,203 + cytR b3934 0.73 +1.4
4,122,247 + cytR b3934 0.77 -4.6
4,122,259 + cytR b3934 0.78 +0.6
4,122,277 - cytR b3934 0.80 +1.0
4,122,277 - cytR b3934 0.80 -1.2
4,122,312 - cytR b3934 0.84 +1.1
4,122,353 + cytR b3934 0.88 +0.8
4,122,353 + cytR b3934 0.88 +0.5
4,122,361 - cytR b3934 0.88 +0.4
4,122,364 + cytR b3934 0.89 +0.2
4,122,387 + -0.1
4,122,395 - +0.9
4,122,499 + -0.0
4,122,502 + +0.1
4,122,647 - +0.3
4,124,262 + priA b3935 0.74 +3.7
4,124,288 - priA b3935 0.75 -0.5
4,124,669 - +2.3
4,124,669 - +1.6
4,124,791 - +1.0
4,124,791 - +2.7
4,124,835 + +1.2
4,124,839 + -0.2
4,124,865 + +0.9
4,124,878 + -1.9
4,124,886 - -0.6
4,124,896 - +0.3
4,124,959 + -2.2
4,125,289 - +1.0
4,125,289 - -0.8
4,125,381 - yiiX b3937 0.12 -0.6
4,125,381 - yiiX b3937 0.12 -2.1
4,125,485 - yiiX b3937 0.29 +0.4
4,125,494 + yiiX b3937 0.30 -0.6
4,125,515 + yiiX b3937 0.34 +1.3
4,125,527 + yiiX b3937 0.36 +0.1
4,125,527 + yiiX b3937 0.36 -0.3
4,125,527 + yiiX b3937 0.36 -1.0
4,125,527 + yiiX b3937 0.36 -0.0
4,125,537 + yiiX b3937 0.37 -0.2
4,125,537 + yiiX b3937 0.37 -0.5
4,125,545 - yiiX b3937 0.39 +1.0
4,125,545 - yiiX b3937 0.39 +0.8
4,125,631 - yiiX b3937 0.53 +1.0
4,125,647 - yiiX b3937 0.56 +0.2
4,125,661 + yiiX b3937 0.58 +0.0
4,125,685 + yiiX b3937 0.62 +0.9
4,125,690 + yiiX b3937 0.63 -1.1
4,125,713 - yiiX b3937 0.66 -1.2
4,125,713 - yiiX b3937 0.66 -3.5
4,125,758 + yiiX b3937 0.74 +0.7
4,125,768 - yiiX b3937 0.75 -0.5
4,125,784 + yiiX b3937 0.78 +0.2
4,125,791 - yiiX b3937 0.79 -0.1
4,125,792 - yiiX b3937 0.79 -2.4
4,125,792 - yiiX b3937 0.79 +1.8

Or see this region's nucleotide sequence