Strain Fitness in Escherichia coli BW25113 around b3351

Experiment: carbon source D-Glucose 20mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntkefB and kefG overlap by 1 nucleotideskefG and yheS are separated by 127 nucleotides b3350: kefB - glutathione-regulated potassium-efflux system protein (NCBI), at 3,476,824 to 3,478,629 kefB b3351: kefG - glutathione-regulated potassium-efflux system ancillary protein (NCBI), at 3,478,629 to 3,479,183 kefG b3352: yheS - fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 3,479,311 to 3,481,224 yheS Position (kb) 3478 3479 3480Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 3477.634 kb on - strand, within kefBat 3477.636 kb on + strand, within kefBat 3477.668 kb on + strand, within kefBat 3477.678 kb on + strand, within kefBat 3477.688 kb on - strand, within kefBat 3477.724 kb on + strand, within kefBat 3477.727 kb on + strand, within kefBat 3477.735 kb on - strand, within kefBat 3477.752 kb on - strand, within kefBat 3477.757 kb on - strand, within kefBat 3477.757 kb on - strand, within kefBat 3477.757 kb on - strand, within kefBat 3477.762 kb on - strand, within kefBat 3477.764 kb on + strand, within kefBat 3477.764 kb on + strand, within kefBat 3477.764 kb on + strand, within kefBat 3477.772 kb on - strand, within kefBat 3477.772 kb on - strand, within kefBat 3477.772 kb on - strand, within kefBat 3477.772 kb on - strand, within kefBat 3477.827 kb on + strand, within kefBat 3477.827 kb on + strand, within kefBat 3477.827 kb on + strand, within kefBat 3477.835 kb on + strand, within kefBat 3477.863 kb on - strand, within kefBat 3477.863 kb on - strand, within kefBat 3477.921 kb on + strand, within kefBat 3477.956 kb on + strand, within kefBat 3477.982 kb on + strand, within kefBat 3477.982 kb on + strand, within kefBat 3477.993 kb on + strand, within kefBat 3478.001 kb on - strand, within kefBat 3478.001 kb on - strand, within kefBat 3478.001 kb on - strand, within kefBat 3478.062 kb on - strand, within kefBat 3478.062 kb on - strand, within kefBat 3478.071 kb on + strand, within kefBat 3478.079 kb on - strand, within kefBat 3478.082 kb on + strand, within kefBat 3478.099 kb on - strand, within kefBat 3478.118 kb on + strand, within kefBat 3478.130 kb on + strand, within kefBat 3478.135 kb on + strand, within kefBat 3478.135 kb on + strand, within kefBat 3478.138 kb on - strand, within kefBat 3478.223 kb on + strand, within kefBat 3478.223 kb on + strand, within kefBat 3478.247 kb on + strand, within kefBat 3478.255 kb on - strand, within kefBat 3478.255 kb on - strand, within kefBat 3478.272 kb on - strand, within kefBat 3478.279 kb on - strand, within kefBat 3478.279 kb on - strand, within kefBat 3478.291 kb on - strand, within kefBat 3478.291 kb on - strand, within kefBat 3478.408 kb on - strand, within kefBat 3478.427 kb on - strand, within kefBat 3478.440 kb on - strand, within kefBat 3478.470 kb on - strandat 3478.494 kb on - strandat 3478.528 kb on + strandat 3478.547 kb on - strandat 3478.576 kb on + strandat 3478.578 kb on - strandat 3478.578 kb on - strandat 3478.637 kb on + strandat 3478.639 kb on + strandat 3478.639 kb on + strandat 3478.669 kb on + strandat 3478.680 kb on + strandat 3478.694 kb on + strand, within kefGat 3478.694 kb on + strand, within kefGat 3478.736 kb on + strand, within kefGat 3478.755 kb on + strand, within kefGat 3478.755 kb on + strand, within kefGat 3478.780 kb on + strand, within kefGat 3478.780 kb on + strand, within kefGat 3478.780 kb on - strand, within kefGat 3478.780 kb on - strand, within kefGat 3478.794 kb on + strand, within kefGat 3478.794 kb on + strand, within kefGat 3478.819 kb on + strand, within kefGat 3478.845 kb on + strand, within kefGat 3478.869 kb on - strand, within kefGat 3478.937 kb on - strand, within kefGat 3478.941 kb on + strand, within kefGat 3478.944 kb on + strand, within kefGat 3478.965 kb on + strand, within kefGat 3478.965 kb on + strand, within kefGat 3478.966 kb on + strand, within kefGat 3478.966 kb on + strand, within kefGat 3478.988 kb on + strand, within kefGat 3478.988 kb on + strand, within kefGat 3479.017 kb on - strand, within kefGat 3479.017 kb on - strand, within kefGat 3479.057 kb on + strand, within kefGat 3479.057 kb on + strand, within kefGat 3479.080 kb on + strand, within kefGat 3479.085 kb on + strand, within kefGat 3479.088 kb on - strand, within kefGat 3479.098 kb on + strand, within kefGat 3479.108 kb on + strand, within kefGat 3479.154 kb on - strandat 3479.170 kb on + strandat 3479.178 kb on - strandat 3479.178 kb on - strandat 3479.205 kb on + strandat 3479.205 kb on + strandat 3479.205 kb on + strandat 3479.305 kb on - strandat 3479.305 kb on - strandat 3479.437 kb on - strandat 3479.437 kb on - strandat 3479.445 kb on - strandat 3479.445 kb on - strandat 3479.486 kb on + strandat 3479.599 kb on + strand, within yheSat 3479.607 kb on - strand, within yheSat 3479.607 kb on - strand, within yheSat 3479.628 kb on - strand, within yheSat 3479.688 kb on - strand, within yheSat 3479.712 kb on - strand, within yheSat 3479.712 kb on - strand, within yheSat 3479.714 kb on + strand, within yheSat 3479.739 kb on - strand, within yheSat 3479.739 kb on - strand, within yheSat 3479.786 kb on + strand, within yheSat 3479.800 kb on - strand, within yheSat 3479.891 kb on + strand, within yheSat 3479.891 kb on + strand, within yheSat 3479.903 kb on + strand, within yheSat 3479.911 kb on - strand, within yheSat 3479.911 kb on - strand, within yheSat 3479.911 kb on - strand, within yheSat 3479.925 kb on - strand, within yheSat 3479.925 kb on - strand, within yheSat 3479.973 kb on + strand, within yheSat 3479.973 kb on + strand, within yheSat 3479.983 kb on + strand, within yheSat 3479.991 kb on - strand, within yheSat 3480.003 kb on - strand, within yheSat 3480.024 kb on - strand, within yheSat 3480.024 kb on - strand, within yheSat 3480.054 kb on + strand, within yheSat 3480.090 kb on + strand, within yheSat 3480.090 kb on + strand, within yheSat 3480.090 kb on - strand, within yheSat 3480.090 kb on - strand, within yheSat 3480.144 kb on - strand, within yheSat 3480.144 kb on - strand, within yheSat 3480.146 kb on + strand, within yheS

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 20mM
remove
3,477,634 - kefB b3350 0.45 +1.0
3,477,636 + kefB b3350 0.45 +0.4
3,477,668 + kefB b3350 0.47 +1.1
3,477,678 + kefB b3350 0.47 -0.8
3,477,688 - kefB b3350 0.48 +1.0
3,477,724 + kefB b3350 0.50 +0.1
3,477,727 + kefB b3350 0.50 +1.0
3,477,735 - kefB b3350 0.50 +0.4
3,477,752 - kefB b3350 0.51 -1.0
3,477,757 - kefB b3350 0.52 +0.9
3,477,757 - kefB b3350 0.52 -1.3
3,477,757 - kefB b3350 0.52 -2.6
3,477,762 - kefB b3350 0.52 +0.6
3,477,764 + kefB b3350 0.52 +1.1
3,477,764 + kefB b3350 0.52 +0.4
3,477,764 + kefB b3350 0.52 +0.6
3,477,772 - kefB b3350 0.52 -0.7
3,477,772 - kefB b3350 0.52 +0.8
3,477,772 - kefB b3350 0.52 +1.1
3,477,772 - kefB b3350 0.52 +0.5
3,477,827 + kefB b3350 0.56 +0.2
3,477,827 + kefB b3350 0.56 +1.0
3,477,827 + kefB b3350 0.56 -0.7
3,477,835 + kefB b3350 0.56 -0.8
3,477,863 - kefB b3350 0.58 -0.5
3,477,863 - kefB b3350 0.58 +0.4
3,477,921 + kefB b3350 0.61 +1.3
3,477,956 + kefB b3350 0.63 -0.3
3,477,982 + kefB b3350 0.64 -0.3
3,477,982 + kefB b3350 0.64 +0.1
3,477,993 + kefB b3350 0.65 +0.5
3,478,001 - kefB b3350 0.65 -1.1
3,478,001 - kefB b3350 0.65 +0.1
3,478,001 - kefB b3350 0.65 +2.0
3,478,062 - kefB b3350 0.69 +0.3
3,478,062 - kefB b3350 0.69 +1.3
3,478,071 + kefB b3350 0.69 +0.6
3,478,079 - kefB b3350 0.69 +0.7
3,478,082 + kefB b3350 0.70 +1.1
3,478,099 - kefB b3350 0.71 +0.9
3,478,118 + kefB b3350 0.72 -1.0
3,478,130 + kefB b3350 0.72 +0.4
3,478,135 + kefB b3350 0.73 +3.1
3,478,135 + kefB b3350 0.73 +0.7
3,478,138 - kefB b3350 0.73 +0.7
3,478,223 + kefB b3350 0.77 -4.5
3,478,223 + kefB b3350 0.77 +1.3
3,478,247 + kefB b3350 0.79 +0.7
3,478,255 - kefB b3350 0.79 -0.7
3,478,255 - kefB b3350 0.79 +1.3
3,478,272 - kefB b3350 0.80 +0.6
3,478,279 - kefB b3350 0.81 -0.1
3,478,279 - kefB b3350 0.81 +0.9
3,478,291 - kefB b3350 0.81 +0.4
3,478,291 - kefB b3350 0.81 +1.4
3,478,408 - kefB b3350 0.88 +0.7
3,478,427 - kefB b3350 0.89 +0.3
3,478,440 - kefB b3350 0.89 -0.3
3,478,470 - +1.1
3,478,494 - +1.4
3,478,528 + +1.0
3,478,547 - +0.7
3,478,576 + -0.4
3,478,578 - -0.1
3,478,578 - +0.9
3,478,637 + +0.3
3,478,639 + -0.6
3,478,639 + +0.6
3,478,669 + -0.1
3,478,680 + +0.7
3,478,694 + kefG b3351 0.12 -0.8
3,478,694 + kefG b3351 0.12 +1.9
3,478,736 + kefG b3351 0.19 +0.7
3,478,755 + kefG b3351 0.23 +0.7
3,478,755 + kefG b3351 0.23 +1.0
3,478,780 + kefG b3351 0.27 +0.2
3,478,780 + kefG b3351 0.27 +1.4
3,478,780 - kefG b3351 0.27 -0.2
3,478,780 - kefG b3351 0.27 -0.7
3,478,794 + kefG b3351 0.30 +0.2
3,478,794 + kefG b3351 0.30 +0.5
3,478,819 + kefG b3351 0.34 +1.1
3,478,845 + kefG b3351 0.39 +0.5
3,478,869 - kefG b3351 0.43 +0.6
3,478,937 - kefG b3351 0.55 -0.3
3,478,941 + kefG b3351 0.56 -0.3
3,478,944 + kefG b3351 0.57 +0.6
3,478,965 + kefG b3351 0.61 +0.0
3,478,965 + kefG b3351 0.61 +1.3
3,478,966 + kefG b3351 0.61 +0.6
3,478,966 + kefG b3351 0.61 -0.5
3,478,988 + kefG b3351 0.65 +1.2
3,478,988 + kefG b3351 0.65 +1.0
3,479,017 - kefG b3351 0.70 +2.9
3,479,017 - kefG b3351 0.70 +2.9
3,479,057 + kefG b3351 0.77 +1.6
3,479,057 + kefG b3351 0.77 +1.5
3,479,080 + kefG b3351 0.81 +1.5
3,479,085 + kefG b3351 0.82 +1.1
3,479,088 - kefG b3351 0.83 +0.8
3,479,098 + kefG b3351 0.85 +0.7
3,479,108 + kefG b3351 0.86 +0.4
3,479,154 - +1.1
3,479,170 + -1.6
3,479,178 - -0.1
3,479,178 - -1.0
3,479,205 + -0.3
3,479,205 + -1.0
3,479,205 + +1.3
3,479,305 - -0.1
3,479,305 - -4.7
3,479,437 - +1.0
3,479,437 - -4.7
3,479,445 - -2.0
3,479,445 - -0.5
3,479,486 + -0.1
3,479,599 + yheS b3352 0.15 -0.3
3,479,607 - yheS b3352 0.15 +0.5
3,479,607 - yheS b3352 0.15 -1.2
3,479,628 - yheS b3352 0.17 -1.2
3,479,688 - yheS b3352 0.20 -4.1
3,479,712 - yheS b3352 0.21 -0.4
3,479,712 - yheS b3352 0.21 +0.6
3,479,714 + yheS b3352 0.21 +0.5
3,479,739 - yheS b3352 0.22 -1.8
3,479,739 - yheS b3352 0.22 -0.1
3,479,786 + yheS b3352 0.25 +0.1
3,479,800 - yheS b3352 0.26 -0.0
3,479,891 + yheS b3352 0.30 +0.6
3,479,891 + yheS b3352 0.30 +0.2
3,479,903 + yheS b3352 0.31 -0.4
3,479,911 - yheS b3352 0.31 +1.3
3,479,911 - yheS b3352 0.31 -3.2
3,479,911 - yheS b3352 0.31 +1.2
3,479,925 - yheS b3352 0.32 +0.9
3,479,925 - yheS b3352 0.32 -1.1
3,479,973 + yheS b3352 0.35 +0.1
3,479,973 + yheS b3352 0.35 -2.0
3,479,983 + yheS b3352 0.35 -0.8
3,479,991 - yheS b3352 0.36 +0.3
3,480,003 - yheS b3352 0.36 +0.3
3,480,024 - yheS b3352 0.37 -0.7
3,480,024 - yheS b3352 0.37 -0.7
3,480,054 + yheS b3352 0.39 +0.1
3,480,090 + yheS b3352 0.41 +0.8
3,480,090 + yheS b3352 0.41 -1.6
3,480,090 - yheS b3352 0.41 -1.3
3,480,090 - yheS b3352 0.41 +0.4
3,480,144 - yheS b3352 0.44 -1.9
3,480,144 - yheS b3352 0.44 -0.1
3,480,146 + yheS b3352 0.44 +1.3

Or see this region's nucleotide sequence