Strain Fitness in Escherichia coli BW25113 around b3341

Experiment: carbon source D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfusA and rpsG are separated by 27 nucleotidesrpsG and rpsL are separated by 96 nucleotidesrpsL and yheL are separated by 125 nucleotidesyheL and yheM are separated by 7 nucleotides b3340: fusA - elongation factor EF-2 (NCBI), at 3,469,422 to 3,471,536 fusA b3341: rpsG - 30S ribosomal protein S7 (NCBI), at 3,471,564 to 3,472,103 rpsG b3342: rpsL - 30S ribosomal protein S12 (NCBI), at 3,472,200 to 3,472,574 rpsL b3343: yheL - predicted intracellular sulfur oxidation protein (NCBI), at 3,472,700 to 3,472,987 yheL b3344: yheM - hypothetical protein (NCBI), at 3,472,995 to 3,473,354 yheM Position (kb) 3471 3472 3473Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3471.616 kb on - strandat 3471.643 kb on - strand, within rpsGat 3471.643 kb on - strand, within rpsGat 3471.645 kb on - strandat 3471.645 kb on - strand, within rpsGat 3472.212 kb on - strandat 3472.640 kb on - strandat 3472.640 kb on - strandat 3472.681 kb on + strandat 3472.681 kb on - strandat 3472.987 kb on - strandat 3472.987 kb on - strandat 3472.999 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 20mM
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3,471,616 - +0.6
3,471,643 - rpsG b3341 0.15 +1.8
3,471,643 - rpsG b3341 0.15 +2.1
3,471,645 - -0.1
3,471,645 - rpsG b3341 0.15 -1.7
3,472,212 - -0.3
3,472,640 - +1.5
3,472,640 - -1.3
3,472,681 + +0.6
3,472,681 - -3.1
3,472,987 - -0.1
3,472,987 - +0.6
3,472,999 - -0.2

Or see this region's nucleotide sequence