Strain Fitness in Escherichia coli BW25113 around b2327

Experiment: carbon source D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb2324 and yfcL are separated by 120 nucleotidesyfcL and yfcM are separated by 33 nucleotidesyfcM and yfcA overlap by 1 nucleotidesyfcA and mepA overlap by 1 nucleotidesmepA and aroC are separated by 3 nucleotides b2324: b2324 - putative peptidase (VIMSS), at 2,439,786 to 2,441,792 b2324 b2325: yfcL - hypothetical protein (NCBI), at 2,441,913 to 2,442,191 yfcL b2326: yfcM - hypothetical protein (NCBI), at 2,442,225 to 2,442,773 yfcM b2327: yfcA - conserved inner membrane protein (NCBI), at 2,442,773 to 2,443,582 yfcA b2328: mepA - penicillin-insensitive murein endopeptidase (NCBI), at 2,443,582 to 2,444,406 mepA b2329: aroC - chorismate synthase (NCBI), at 2,444,410 to 2,445,495 aroC Position (kb) 2442 2443 2444Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2441.839 kb on + strandat 2441.883 kb on + strandat 2441.904 kb on - strandat 2441.966 kb on + strand, within yfcLat 2441.966 kb on + strand, within yfcLat 2442.015 kb on + strand, within yfcLat 2442.015 kb on + strand, within yfcLat 2442.033 kb on - strand, within yfcLat 2442.050 kb on + strand, within yfcLat 2442.050 kb on + strand, within yfcLat 2442.200 kb on - strandat 2442.221 kb on + strandat 2442.316 kb on - strand, within yfcMat 2442.343 kb on + strand, within yfcMat 2442.357 kb on - strand, within yfcMat 2442.440 kb on + strand, within yfcMat 2442.522 kb on + strand, within yfcMat 2442.583 kb on - strand, within yfcMat 2442.647 kb on - strand, within yfcMat 2442.647 kb on - strand, within yfcMat 2442.696 kb on - strand, within yfcMat 2442.758 kb on + strandat 2442.758 kb on + strandat 2442.758 kb on + strandat 2442.766 kb on - strandat 2442.766 kb on - strandat 2442.766 kb on - strandat 2442.766 kb on - strandat 2442.777 kb on - strandat 2442.807 kb on - strandat 2442.887 kb on + strand, within yfcAat 2442.887 kb on + strand, within yfcAat 2442.887 kb on + strand, within yfcAat 2442.887 kb on + strand, within yfcAat 2442.898 kb on + strand, within yfcAat 2442.906 kb on - strand, within yfcAat 2442.906 kb on - strand, within yfcAat 2442.948 kb on - strand, within yfcAat 2442.976 kb on + strand, within yfcAat 2442.985 kb on + strand, within yfcAat 2443.071 kb on - strand, within yfcAat 2443.161 kb on + strand, within yfcAat 2443.293 kb on - strand, within yfcAat 2443.330 kb on + strand, within yfcAat 2443.700 kb on - strand, within mepAat 2443.700 kb on - strand, within mepAat 2443.705 kb on + strand, within mepAat 2443.770 kb on + strand, within mepAat 2443.778 kb on - strand, within mepAat 2443.778 kb on - strand, within mepAat 2443.782 kb on - strand, within mepAat 2443.797 kb on - strand, within mepAat 2443.797 kb on - strand, within mepAat 2443.817 kb on - strand, within mepAat 2443.817 kb on - strand, within mepAat 2443.897 kb on + strand, within mepAat 2444.082 kb on - strand, within mepAat 2444.245 kb on - strand, within mepAat 2444.245 kb on - strand, within mepAat 2444.256 kb on + strand, within mepAat 2444.552 kb on - strand, within aroC

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 20mM
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2,441,839 + +0.1
2,441,883 + -0.8
2,441,904 - +0.5
2,441,966 + yfcL b2325 0.19 -2.9
2,441,966 + yfcL b2325 0.19 -2.9
2,442,015 + yfcL b2325 0.37 +1.0
2,442,015 + yfcL b2325 0.37 +2.3
2,442,033 - yfcL b2325 0.43 -0.2
2,442,050 + yfcL b2325 0.49 -2.4
2,442,050 + yfcL b2325 0.49 -2.0
2,442,200 - +3.0
2,442,221 + +1.1
2,442,316 - yfcM b2326 0.17 +1.4
2,442,343 + yfcM b2326 0.21 -0.5
2,442,357 - yfcM b2326 0.24 +0.3
2,442,440 + yfcM b2326 0.39 +0.1
2,442,522 + yfcM b2326 0.54 -1.0
2,442,583 - yfcM b2326 0.65 +0.8
2,442,647 - yfcM b2326 0.77 -0.0
2,442,647 - yfcM b2326 0.77 -0.1
2,442,696 - yfcM b2326 0.86 -1.9
2,442,758 + +0.5
2,442,758 + -0.1
2,442,758 + +0.1
2,442,766 - -0.6
2,442,766 - +0.1
2,442,766 - +0.2
2,442,766 - -1.8
2,442,777 - -0.6
2,442,807 - +0.1
2,442,887 + yfcA b2327 0.14 +1.7
2,442,887 + yfcA b2327 0.14 -0.9
2,442,887 + yfcA b2327 0.14 -0.2
2,442,887 + yfcA b2327 0.14 +2.1
2,442,898 + yfcA b2327 0.15 +1.1
2,442,906 - yfcA b2327 0.16 +2.5
2,442,906 - yfcA b2327 0.16 -1.3
2,442,948 - yfcA b2327 0.22 +0.2
2,442,976 + yfcA b2327 0.25 +0.8
2,442,985 + yfcA b2327 0.26 +1.2
2,443,071 - yfcA b2327 0.37 +1.9
2,443,161 + yfcA b2327 0.48 -0.1
2,443,293 - yfcA b2327 0.64 +0.8
2,443,330 + yfcA b2327 0.69 +0.7
2,443,700 - mepA b2328 0.14 +0.1
2,443,700 - mepA b2328 0.14 -0.7
2,443,705 + mepA b2328 0.15 -0.8
2,443,770 + mepA b2328 0.23 -0.2
2,443,778 - mepA b2328 0.24 +0.6
2,443,778 - mepA b2328 0.24 -2.9
2,443,782 - mepA b2328 0.24 +0.7
2,443,797 - mepA b2328 0.26 -2.3
2,443,797 - mepA b2328 0.26 -0.7
2,443,817 - mepA b2328 0.28 +0.3
2,443,817 - mepA b2328 0.28 +0.5
2,443,897 + mepA b2328 0.38 +0.7
2,444,082 - mepA b2328 0.61 -0.4
2,444,245 - mepA b2328 0.80 -0.9
2,444,245 - mepA b2328 0.80 +1.1
2,444,256 + mepA b2328 0.82 -0.1
2,444,552 - aroC b2329 0.13 -3.0

Or see this region's nucleotide sequence