Strain Fitness in Escherichia coli BW25113 around b2020

Experiment: carbon source D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisG and hisD are separated by 5 nucleotideshisD and hisC overlap by 4 nucleotides b2019: hisG - ATP phosphoribosyltransferase (NCBI), at 2,088,216 to 2,089,115 hisG b2020: hisD - histidinol dehydrogenase (NCBI), at 2,089,121 to 2,090,425 hisD b2021: hisC - histidinol-phosphate aminotransferase (NCBI), at 2,090,422 to 2,091,492 hisC Position (kb) 2089 2090 2091Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1 2at 2088.203 kb on - strandat 2088.203 kb on - strandat 2088.316 kb on + strand, within hisGat 2088.484 kb on - strand, within hisGat 2088.602 kb on + strand, within hisGat 2088.610 kb on - strand, within hisGat 2088.610 kb on - strand, within hisGat 2088.657 kb on - strand, within hisGat 2088.681 kb on - strand, within hisGat 2088.859 kb on + strand, within hisGat 2088.900 kb on + strand, within hisGat 2088.900 kb on + strand, within hisGat 2089.029 kb on + strandat 2089.104 kb on + strandat 2089.133 kb on - strandat 2089.155 kb on - strandat 2089.187 kb on - strandat 2089.234 kb on - strandat 2089.234 kb on - strandat 2089.267 kb on + strand, within hisDat 2089.326 kb on + strand, within hisDat 2089.419 kb on - strand, within hisDat 2089.504 kb on + strand, within hisDat 2089.605 kb on - strand, within hisDat 2089.755 kb on - strand, within hisDat 2089.776 kb on + strand, within hisDat 2089.844 kb on + strand, within hisDat 2089.929 kb on - strand, within hisDat 2089.929 kb on - strand, within hisDat 2090.018 kb on - strand, within hisDat 2090.018 kb on - strand, within hisDat 2090.075 kb on - strand, within hisDat 2090.124 kb on + strand, within hisDat 2090.124 kb on + strand, within hisDat 2090.132 kb on - strand, within hisDat 2090.300 kb on - strandat 2090.337 kb on - strandat 2090.419 kb on + strandat 2090.721 kb on - strand, within hisCat 2090.808 kb on - strand, within hisCat 2090.932 kb on + strand, within hisCat 2090.932 kb on + strand, within hisCat 2090.938 kb on + strand, within hisCat 2091.102 kb on + strand, within hisCat 2091.202 kb on - strand, within hisCat 2091.202 kb on - strand, within hisCat 2091.274 kb on + strand, within hisCat 2091.351 kb on + strand, within hisC

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 20mM
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2,088,203 - -0.4
2,088,203 - -3.9
2,088,316 + hisG b2019 0.11 -4.6
2,088,484 - hisG b2019 0.30 -5.5
2,088,602 + hisG b2019 0.43 -4.0
2,088,610 - hisG b2019 0.44 -2.7
2,088,610 - hisG b2019 0.44 -5.9
2,088,657 - hisG b2019 0.49 -3.4
2,088,681 - hisG b2019 0.52 -2.1
2,088,859 + hisG b2019 0.71 +0.1
2,088,900 + hisG b2019 0.76 -2.0
2,088,900 + hisG b2019 0.76 -1.5
2,089,029 + -4.6
2,089,104 + -1.8
2,089,133 - -6.1
2,089,155 - -1.9
2,089,187 - -2.2
2,089,234 - -3.4
2,089,234 - -4.8
2,089,267 + hisD b2020 0.11 -5.0
2,089,326 + hisD b2020 0.16 -4.9
2,089,419 - hisD b2020 0.23 -3.8
2,089,504 + hisD b2020 0.29 -3.7
2,089,605 - hisD b2020 0.37 -3.9
2,089,755 - hisD b2020 0.49 -5.6
2,089,776 + hisD b2020 0.50 -6.8
2,089,844 + hisD b2020 0.55 -4.2
2,089,929 - hisD b2020 0.62 -4.3
2,089,929 - hisD b2020 0.62 -5.6
2,090,018 - hisD b2020 0.69 -2.9
2,090,018 - hisD b2020 0.69 -4.4
2,090,075 - hisD b2020 0.73 -2.9
2,090,124 + hisD b2020 0.77 -5.7
2,090,124 + hisD b2020 0.77 -5.2
2,090,132 - hisD b2020 0.77 -5.9
2,090,300 - -2.9
2,090,337 - -5.5
2,090,419 + +2.0
2,090,721 - hisC b2021 0.28 -2.7
2,090,808 - hisC b2021 0.36 -4.0
2,090,932 + hisC b2021 0.48 -5.3
2,090,932 + hisC b2021 0.48 -4.8
2,090,938 + hisC b2021 0.48 -4.0
2,091,102 + hisC b2021 0.63 -5.5
2,091,202 - hisC b2021 0.73 -2.8
2,091,202 - hisC b2021 0.73 -2.6
2,091,274 + hisC b2021 0.80 -3.9
2,091,351 + hisC b2021 0.87 -6.3

Or see this region's nucleotide sequence