Strain Fitness in Escherichia coli BW25113 around b1271

Experiment: carbon source D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrluB and btuR are separated by 39 nucleotidesbtuR and yciK overlap by 4 nucleotidesyciK and sohB are separated by 219 nucleotides b1269: rluB - 23S rRNA pseudouridylate synthase (NCBI), at 1,324,876 to 1,325,751 rluB b1270: btuR - cob(I)yrinic acid a,c-diamide adenosyltransferase (NCBI), at 1,325,791 to 1,326,381 btuR b1271: yciK - short chain dehydrogenase (NCBI), at 1,326,378 to 1,327,136 yciK b1272: sohB - predicted inner membrane peptidase (NCBI), at 1,327,356 to 1,328,405 sohB Position (kb) 1326 1327 1328Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1325.635 kb on - strand, within rluBat 1325.636 kb on - strand, within rluBat 1325.636 kb on - strand, within rluBat 1325.814 kb on + strandat 1325.882 kb on - strand, within btuRat 1325.954 kb on - strand, within btuRat 1326.099 kb on - strand, within btuRat 1326.099 kb on - strand, within btuRat 1326.296 kb on - strand, within btuRat 1326.420 kb on + strandat 1326.420 kb on + strandat 1326.546 kb on + strand, within yciKat 1326.719 kb on + strand, within yciKat 1326.742 kb on - strand, within yciKat 1326.851 kb on - strand, within yciKat 1327.275 kb on + strandat 1327.297 kb on + strandat 1327.500 kb on + strand, within sohBat 1327.505 kb on + strand, within sohBat 1327.505 kb on + strand, within sohBat 1327.511 kb on - strand, within sohBat 1327.511 kb on - strand, within sohBat 1327.565 kb on + strand, within sohBat 1327.565 kb on + strand, within sohBat 1327.752 kb on - strand, within sohBat 1327.752 kb on - strand, within sohBat 1327.797 kb on + strand, within sohBat 1327.797 kb on + strand, within sohBat 1327.902 kb on - strand, within sohBat 1328.003 kb on + strand, within sohBat 1328.032 kb on - strand, within sohBat 1328.039 kb on + strand, within sohBat 1328.039 kb on + strand, within sohB

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Glucose 20mM
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1,325,635 - rluB b1269 0.87 -1.8
1,325,636 - rluB b1269 0.87 +0.4
1,325,636 - rluB b1269 0.87 -1.5
1,325,814 + -3.9
1,325,882 - btuR b1270 0.15 -0.7
1,325,954 - btuR b1270 0.28 +0.1
1,326,099 - btuR b1270 0.52 -1.8
1,326,099 - btuR b1270 0.52 +0.2
1,326,296 - btuR b1270 0.85 +0.6
1,326,420 + +1.1
1,326,420 + +0.0
1,326,546 + yciK b1271 0.22 +1.3
1,326,719 + yciK b1271 0.45 +1.9
1,326,742 - yciK b1271 0.48 -0.1
1,326,851 - yciK b1271 0.62 +0.3
1,327,275 + -2.8
1,327,297 + -0.6
1,327,500 + sohB b1272 0.14 +0.1
1,327,505 + sohB b1272 0.14 -0.5
1,327,505 + sohB b1272 0.14 -0.3
1,327,511 - sohB b1272 0.15 -2.1
1,327,511 - sohB b1272 0.15 +1.1
1,327,565 + sohB b1272 0.20 +0.7
1,327,565 + sohB b1272 0.20 -1.8
1,327,752 - sohB b1272 0.38 +1.2
1,327,752 - sohB b1272 0.38 +1.0
1,327,797 + sohB b1272 0.42 +0.9
1,327,797 + sohB b1272 0.42 +1.2
1,327,902 - sohB b1272 0.52 +0.5
1,328,003 + sohB b1272 0.62 +0.7
1,328,032 - sohB b1272 0.64 +0.6
1,328,039 + sohB b1272 0.65 -1.3
1,328,039 + sohB b1272 0.65 +0.8

Or see this region's nucleotide sequence