Strain Fitness in Escherichia coli BW25113 around b3652

Experiment: carbon source D-Mannose 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntspoT and trmH are separated by 6 nucleotidestrmH and recG are separated by 5 nucleotidesrecG and gltS are separated by 168 nucleotides b3650: spoT - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI), at 3,820,423 to 3,822,531 spoT b3651: trmH - tRNA (Guanosine-2'-O-)-methyltransferase (NCBI), at 3,822,538 to 3,823,227 trmH b3652: recG - ATP-dependent DNA helicase (NCBI), at 3,823,233 to 3,825,314 recG b3653: gltS - glutamate transporter (NCBI), at 3,825,483 to 3,826,688 gltS Position (kb) 3823 3824 3825 3826Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4at 3822.244 kb on + strand, within spoTat 3822.263 kb on - strand, within spoTat 3822.263 kb on - strand, within spoTat 3822.397 kb on - strandat 3822.397 kb on - strandat 3822.401 kb on - strandat 3822.409 kb on - strandat 3822.409 kb on - strandat 3822.417 kb on + strandat 3822.455 kb on + strandat 3822.455 kb on + strandat 3822.493 kb on + strandat 3822.493 kb on + strandat 3822.592 kb on + strandat 3822.615 kb on + strand, within trmHat 3822.615 kb on + strand, within trmHat 3822.615 kb on + strand, within trmHat 3822.618 kb on - strand, within trmHat 3822.635 kb on - strand, within trmHat 3822.733 kb on + strand, within trmHat 3822.746 kb on + strand, within trmHat 3822.746 kb on + strand, within trmHat 3822.749 kb on - strand, within trmHat 3822.761 kb on - strand, within trmHat 3822.778 kb on - strand, within trmHat 3822.796 kb on - strand, within trmHat 3822.799 kb on + strand, within trmHat 3822.799 kb on + strand, within trmHat 3822.807 kb on - strand, within trmHat 3822.922 kb on - strand, within trmHat 3822.930 kb on - strand, within trmHat 3822.930 kb on - strand, within trmHat 3822.945 kb on - strand, within trmHat 3823.040 kb on - strand, within trmHat 3823.089 kb on - strand, within trmHat 3823.117 kb on - strand, within trmHat 3823.179 kb on + strandat 3823.179 kb on + strandat 3823.189 kb on + strandat 3823.189 kb on + strandat 3823.215 kb on - strandat 3823.215 kb on - strandat 3823.231 kb on - strandat 3823.265 kb on + strandat 3823.267 kb on + strandat 3823.285 kb on + strandat 3823.341 kb on + strandat 3823.341 kb on + strandat 3823.379 kb on + strandat 3823.379 kb on + strandat 3823.387 kb on - strandat 3823.387 kb on - strandat 3823.393 kb on + strandat 3823.439 kb on + strandat 3823.482 kb on - strand, within recGat 3823.562 kb on - strand, within recGat 3823.574 kb on + strand, within recGat 3823.582 kb on - strand, within recGat 3823.629 kb on - strand, within recGat 3823.641 kb on + strand, within recGat 3823.649 kb on + strand, within recGat 3823.662 kb on - strand, within recGat 3823.690 kb on - strand, within recGat 3823.690 kb on - strand, within recGat 3823.751 kb on + strand, within recGat 3823.751 kb on + strand, within recGat 3823.776 kb on - strand, within recGat 3823.793 kb on + strand, within recGat 3823.805 kb on + strand, within recGat 3823.823 kb on + strand, within recGat 3823.831 kb on - strand, within recGat 3823.881 kb on - strand, within recGat 3823.883 kb on + strand, within recGat 3823.925 kb on - strand, within recGat 3823.969 kb on + strand, within recGat 3824.052 kb on - strand, within recGat 3824.052 kb on - strand, within recGat 3824.061 kb on + strand, within recGat 3824.085 kb on + strand, within recGat 3824.088 kb on - strand, within recGat 3824.112 kb on + strand, within recGat 3824.142 kb on - strand, within recGat 3824.163 kb on - strand, within recGat 3824.193 kb on + strand, within recGat 3824.228 kb on - strand, within recGat 3824.261 kb on + strand, within recGat 3824.274 kb on - strand, within recGat 3824.302 kb on - strand, within recGat 3824.316 kb on + strand, within recGat 3824.316 kb on + strand, within recGat 3824.329 kb on - strand, within recGat 3824.329 kb on - strand, within recGat 3824.346 kb on + strand, within recGat 3824.409 kb on - strand, within recGat 3824.414 kb on - strand, within recGat 3824.414 kb on - strand, within recGat 3824.434 kb on + strand, within recGat 3824.444 kb on + strand, within recGat 3824.444 kb on + strand, within recGat 3824.450 kb on - strand, within recGat 3824.594 kb on - strand, within recGat 3824.609 kb on - strand, within recGat 3824.653 kb on + strand, within recGat 3824.658 kb on + strand, within recGat 3824.661 kb on - strand, within recGat 3824.666 kb on + strand, within recGat 3824.681 kb on - strand, within recGat 3824.681 kb on - strand, within recGat 3824.751 kb on + strand, within recGat 3824.793 kb on + strand, within recGat 3824.799 kb on + strand, within recGat 3824.799 kb on + strand, within recGat 3824.868 kb on - strand, within recGat 3824.876 kb on + strand, within recGat 3824.878 kb on + strand, within recGat 3824.923 kb on - strand, within recGat 3824.983 kb on + strand, within recGat 3824.983 kb on + strand, within recGat 3825.011 kb on - strand, within recGat 3825.011 kb on - strand, within recGat 3825.013 kb on - strand, within recGat 3825.038 kb on + strand, within recGat 3825.038 kb on + strand, within recGat 3825.218 kb on + strandat 3825.253 kb on - strandat 3825.334 kb on - strandat 3825.334 kb on - strandat 3825.354 kb on - strandat 3825.364 kb on - strandat 3825.414 kb on + strandat 3825.505 kb on + strandat 3825.505 kb on + strandat 3825.593 kb on + strandat 3825.593 kb on + strandat 3825.602 kb on + strandat 3825.650 kb on + strand, within gltSat 3825.711 kb on - strand, within gltSat 3825.716 kb on - strand, within gltSat 3825.756 kb on - strand, within gltSat 3825.803 kb on + strand, within gltSat 3825.834 kb on - strand, within gltSat 3825.850 kb on - strand, within gltSat 3825.855 kb on - strand, within gltSat 3825.860 kb on - strand, within gltSat 3825.870 kb on - strand, within gltSat 3825.932 kb on + strand, within gltSat 3825.932 kb on + strand, within gltSat 3826.037 kb on - strand, within gltSat 3826.070 kb on - strand, within gltSat 3826.140 kb on - strand, within gltSat 3826.173 kb on + strand, within gltSat 3826.173 kb on + strand, within gltSat 3826.208 kb on - strand, within gltSat 3826.212 kb on - strand, within gltSat 3826.273 kb on - strand, within gltSat 3826.275 kb on - strand, within gltSat 3826.289 kb on + strand, within gltSat 3826.297 kb on - strand, within gltS

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source D-Mannose 5mM
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3,822,244 + spoT b3650 0.86 +0.4
3,822,263 - spoT b3650 0.87 -0.3
3,822,263 - spoT b3650 0.87 +0.1
3,822,397 - -1.5
3,822,397 - -0.6
3,822,401 - -1.3
3,822,409 - -2.3
3,822,409 - +1.8
3,822,417 + -2.8
3,822,455 + -3.3
3,822,455 + -2.4
3,822,493 + +0.5
3,822,493 + +1.1
3,822,592 + +1.9
3,822,615 + trmH b3651 0.11 -0.5
3,822,615 + trmH b3651 0.11 +1.1
3,822,615 + trmH b3651 0.11 +1.5
3,822,618 - trmH b3651 0.12 +0.7
3,822,635 - trmH b3651 0.14 -1.9
3,822,733 + trmH b3651 0.28 +0.4
3,822,746 + trmH b3651 0.30 -0.5
3,822,746 + trmH b3651 0.30 -0.4
3,822,749 - trmH b3651 0.31 +1.1
3,822,761 - trmH b3651 0.32 +0.3
3,822,778 - trmH b3651 0.35 +1.6
3,822,796 - trmH b3651 0.37 +0.9
3,822,799 + trmH b3651 0.38 +0.4
3,822,799 + trmH b3651 0.38 -1.0
3,822,807 - trmH b3651 0.39 +0.4
3,822,922 - trmH b3651 0.56 +1.1
3,822,930 - trmH b3651 0.57 +1.7
3,822,930 - trmH b3651 0.57 -3.2
3,822,945 - trmH b3651 0.59 +2.7
3,823,040 - trmH b3651 0.73 -0.4
3,823,089 - trmH b3651 0.80 +0.1
3,823,117 - trmH b3651 0.84 +0.9
3,823,179 + -1.7
3,823,179 + +0.9
3,823,189 + -1.6
3,823,189 + +2.6
3,823,215 - -0.1
3,823,215 - -0.4
3,823,231 - -1.0
3,823,265 + +0.4
3,823,267 + +2.6
3,823,285 + +1.5
3,823,341 + +1.4
3,823,341 + +4.6
3,823,379 + +0.9
3,823,379 + +0.6
3,823,387 - +0.3
3,823,387 - +1.2
3,823,393 + +0.3
3,823,439 + +1.2
3,823,482 - recG b3652 0.12 +0.3
3,823,562 - recG b3652 0.16 +1.4
3,823,574 + recG b3652 0.16 -1.7
3,823,582 - recG b3652 0.17 -0.8
3,823,629 - recG b3652 0.19 +0.4
3,823,641 + recG b3652 0.20 +2.9
3,823,649 + recG b3652 0.20 -0.9
3,823,662 - recG b3652 0.21 +2.3
3,823,690 - recG b3652 0.22 -2.1
3,823,690 - recG b3652 0.22 +1.0
3,823,751 + recG b3652 0.25 +1.2
3,823,751 + recG b3652 0.25 -1.0
3,823,776 - recG b3652 0.26 -1.0
3,823,793 + recG b3652 0.27 +4.0
3,823,805 + recG b3652 0.27 +0.5
3,823,823 + recG b3652 0.28 +0.6
3,823,831 - recG b3652 0.29 -2.2
3,823,881 - recG b3652 0.31 -1.1
3,823,883 + recG b3652 0.31 +0.6
3,823,925 - recG b3652 0.33 -0.2
3,823,969 + recG b3652 0.35 +1.6
3,824,052 - recG b3652 0.39 +1.7
3,824,052 - recG b3652 0.39 -2.5
3,824,061 + recG b3652 0.40 +1.0
3,824,085 + recG b3652 0.41 -1.0
3,824,088 - recG b3652 0.41 +1.3
3,824,112 + recG b3652 0.42 +2.8
3,824,142 - recG b3652 0.44 -2.4
3,824,163 - recG b3652 0.45 -0.9
3,824,193 + recG b3652 0.46 +1.7
3,824,228 - recG b3652 0.48 +0.5
3,824,261 + recG b3652 0.49 +2.3
3,824,274 - recG b3652 0.50 +0.1
3,824,302 - recG b3652 0.51 +1.1
3,824,316 + recG b3652 0.52 +1.8
3,824,316 + recG b3652 0.52 +0.5
3,824,329 - recG b3652 0.53 -1.0
3,824,329 - recG b3652 0.53 +0.4
3,824,346 + recG b3652 0.53 +1.4
3,824,409 - recG b3652 0.56 -0.7
3,824,414 - recG b3652 0.57 -2.0
3,824,414 - recG b3652 0.57 +4.5
3,824,434 + recG b3652 0.58 +0.9
3,824,444 + recG b3652 0.58 +1.9
3,824,444 + recG b3652 0.58 +0.5
3,824,450 - recG b3652 0.58 +1.4
3,824,594 - recG b3652 0.65 -1.3
3,824,609 - recG b3652 0.66 -1.9
3,824,653 + recG b3652 0.68 -0.1
3,824,658 + recG b3652 0.68 +1.4
3,824,661 - recG b3652 0.69 -1.4
3,824,666 + recG b3652 0.69 +0.3
3,824,681 - recG b3652 0.70 +1.6
3,824,681 - recG b3652 0.70 +1.4
3,824,751 + recG b3652 0.73 +0.6
3,824,793 + recG b3652 0.75 +1.8
3,824,799 + recG b3652 0.75 -0.9
3,824,799 + recG b3652 0.75 -1.9
3,824,868 - recG b3652 0.79 -2.3
3,824,876 + recG b3652 0.79 +1.1
3,824,878 + recG b3652 0.79 +1.0
3,824,923 - recG b3652 0.81 -0.4
3,824,983 + recG b3652 0.84 -1.9
3,824,983 + recG b3652 0.84 +2.2
3,825,011 - recG b3652 0.85 -1.1
3,825,011 - recG b3652 0.85 +1.2
3,825,013 - recG b3652 0.85 +1.6
3,825,038 + recG b3652 0.87 -0.6
3,825,038 + recG b3652 0.87 -2.4
3,825,218 + +0.2
3,825,253 - +0.3
3,825,334 - +0.1
3,825,334 - -0.7
3,825,354 - +0.2
3,825,364 - +0.3
3,825,414 + +0.9
3,825,505 + -0.8
3,825,505 + +0.2
3,825,593 + +0.6
3,825,593 + -1.2
3,825,602 + +1.1
3,825,650 + gltS b3653 0.14 -2.2
3,825,711 - gltS b3653 0.19 +0.8
3,825,716 - gltS b3653 0.19 -0.2
3,825,756 - gltS b3653 0.23 +1.0
3,825,803 + gltS b3653 0.27 +1.3
3,825,834 - gltS b3653 0.29 -0.6
3,825,850 - gltS b3653 0.30 -0.1
3,825,855 - gltS b3653 0.31 -0.6
3,825,860 - gltS b3653 0.31 +0.6
3,825,870 - gltS b3653 0.32 +0.8
3,825,932 + gltS b3653 0.37 +0.5
3,825,932 + gltS b3653 0.37 -0.9
3,826,037 - gltS b3653 0.46 +0.7
3,826,070 - gltS b3653 0.49 -5.1
3,826,140 - gltS b3653 0.54 +0.5
3,826,173 + gltS b3653 0.57 +1.8
3,826,173 + gltS b3653 0.57 +0.2
3,826,208 - gltS b3653 0.60 -0.2
3,826,212 - gltS b3653 0.60 +0.2
3,826,273 - gltS b3653 0.66 -0.1
3,826,275 - gltS b3653 0.66 -0.1
3,826,289 + gltS b3653 0.67 +1.5
3,826,297 - gltS b3653 0.67 +0.0

Or see this region's nucleotide sequence